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Title: Cytokinin Signaling in Mycobacterium tuberculosis

Abstract

It was recently reported that the human-exclusive pathogenMycobacterium tuberculosissecretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life.M. tuberculosisis not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of theM. tuberculosisgene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining ofM. tuberculosis. While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affectM. tuberculosisacid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. IMPORTANCECytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in allmore » domains of life.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1461690
Resource Type:
Journal Article
Journal Name:
mBio (Online)
Additional Journal Information:
Journal Volume: 9; Journal Issue: 3; Journal ID: ISSN 2150-7511
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Samanovic, Marie I., Hsu, Hao-Chi, Jones, Marcus B., Jones, Victoria, McNeil, Michael R., Becker, Samuel H., Jordan, Ashley T., Strnad, Miroslav, Xu, Changcheng, Jackson, Mary, Li, Huilin, Darwin, K. Heran, Sherman, David R., and Greenberg, E. Peter. Cytokinin Signaling in Mycobacterium tuberculosis. United States: N. p., 2018. Web. doi:10.1128/mBio.00989-18.
Samanovic, Marie I., Hsu, Hao-Chi, Jones, Marcus B., Jones, Victoria, McNeil, Michael R., Becker, Samuel H., Jordan, Ashley T., Strnad, Miroslav, Xu, Changcheng, Jackson, Mary, Li, Huilin, Darwin, K. Heran, Sherman, David R., & Greenberg, E. Peter. Cytokinin Signaling in Mycobacterium tuberculosis. United States. doi:10.1128/mBio.00989-18.
Samanovic, Marie I., Hsu, Hao-Chi, Jones, Marcus B., Jones, Victoria, McNeil, Michael R., Becker, Samuel H., Jordan, Ashley T., Strnad, Miroslav, Xu, Changcheng, Jackson, Mary, Li, Huilin, Darwin, K. Heran, Sherman, David R., and Greenberg, E. Peter. Tue . "Cytokinin Signaling in Mycobacterium tuberculosis". United States. doi:10.1128/mBio.00989-18.
@article{osti_1461690,
title = {Cytokinin Signaling in Mycobacterium tuberculosis},
author = {Samanovic, Marie I. and Hsu, Hao-Chi and Jones, Marcus B. and Jones, Victoria and McNeil, Michael R. and Becker, Samuel H. and Jordan, Ashley T. and Strnad, Miroslav and Xu, Changcheng and Jackson, Mary and Li, Huilin and Darwin, K. Heran and Sherman, David R. and Greenberg, E. Peter},
abstractNote = {It was recently reported that the human-exclusive pathogenMycobacterium tuberculosissecretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life.M. tuberculosisis not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of theM. tuberculosisgene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining ofM. tuberculosis. While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affectM. tuberculosisacid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. IMPORTANCECytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.},
doi = {10.1128/mBio.00989-18},
journal = {mBio (Online)},
issn = {2150-7511},
number = 3,
volume = 9,
place = {United States},
year = {2018},
month = {6}
}

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