Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico

Johnson, D. B.; Beddows, P. A.; Flynn, T. M.; Osburn, M. R.

doi:10.1111/gbi.12283

Title: Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico

Journal Article · Sun Apr 15 00:00:00 EDT 2018 · Geobiology

DOI:https://doi.org/10.1111/gbi.12283· OSTI ID:1461427

Johnson, D. B. ^[1]; Beddows, P. A. ^[1]; Flynn, T. M. ^[2]; Osburn, M. R. ^[1]

Northwestern Univ., Evanston, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

Laguna Bacalar is a sulfate-rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. We report that while cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three-pronged geobiological approach of microscopy, high-throughput DNA sequencing, and lipid biomarker analyses. We identify and compare diverse microbial communities of Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria that vary with location along a bank-to-bank transect across the lake, within microbialites, and within a neighboring mangrove root agglomeration. In particular, sulfate-reducing bacteria are extremely common and diverse, constituting 7%-19% of phylogenetic diversity within the microbialites, and are hypothesized to significantly influence carbonate precipitation. In contrast, Cyanobacteria account for less than 1% of phylogenetic diversity. The distribution of lipid biomarkers reflects these changes in microbial ecology, providing meaningful biosignatures for the microbes in this system. Polysaturated short-chain fatty acids characteristic of cyanobacteria account for <3% of total abundance in Laguna Bacalar microbialites. By contrast, even short-chain and monounsaturated short-chain fatty acids attributable to both Cyanobacteria and many other organisms including types of Alphaproteobacteria and Gammaproteobacteria constitute 43%-69% and 17%-25%, respectively, of total abundance in microbialites. Lastly, while cyanobacteria are the largest and most visible microbes within these microbialites and dominate the mangrove root agglomeration, it is clear that their smaller, metabolically diverse associates are responsible for significant biogeochemical cycling in this microbialite system.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

Contributing Organization:: Northwestern University, Evanston, IL (United States)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1461427

Journal Information:: Geobiology, Vol. 16, Issue 3; ISSN 1472-4677

Publisher:: WilieyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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References (94)

Microbialites: Organosedimentary Deposits of Benthic Microbial Communities Burne, Robert V.; Moore, Linda S. PALAIOS, Vol. 2, Issue 3 https://doi.org/10.2307/3514674	journal	January 1987
Hydrochemistry and microbialites of the alkaline crater lake Alchichica, Mexico Kaźmierczak, Józef; Kempe, Stephan; Kremer, Barbara Facies, Vol. 57, Issue 4 https://doi.org/10.1007/s10347-010-0255-8	journal	January 2011
STROMATOLITES IN PRECAMBRIAN CARBONATES: Evolutionary Mileposts or Environmental Dipsticks? Grotzinger, John P.; Knoll, Andrew H. Annual Review of Earth and Planetary Sciences, Vol. 27, Issue 1 https://doi.org/10.1146/annurev.earth.27.1.313	journal	May 1999
Rapid emergence of life shown by discovery of 3,700-million-year-old microbial structures Nutman, Allen P.; Bennett, Vickie C.; Friend, Clark R. L. Nature, Vol. 537, Issue 7621 https://doi.org/10.1038/nature19355	journal	August 2016
Water chemistry and CaCO3 dissolution in the saline part of an open-flow mixing zone, coastal Yucatan Peninsula, Mexico Stoessell, R. K.; Ward, W. C.; Ford, B. H. Geological Society of America Bulletin, Vol. 101, Issue 2 https://doi.org/10.1130/0016-7606(1989)101<0159:WCACDI>2.3.CO;2	journal	February 1989
The biosynthesis of cyanobacterial sunscreen scytonemin in intertidal microbial mat communities: Scytonemin biosynthesis in intertidal microbial mats Balskus, Emily P.; Case, Rebecca J.; Walsh, Christopher T. FEMS Microbiology Ecology, Vol. 77, Issue 2 https://doi.org/10.1111/j.1574-6941.2011.01113.x	journal	May 2011
Microbial lithification in marine stromatolites and hypersaline mats Dupraz, Christophe; Visscher, Pieter T. Trends in Microbiology, Vol. 13, Issue 9 https://doi.org/10.1016/j.tim.2005.07.008	journal	September 2005
Assessment of Bias Associated with Incomplete Extraction of Microbial DNA from Soil Feinstein, L. M.; Sul, W. J.; Blackwood, C. B. Applied and Environmental Microbiology, Vol. 75, Issue 16 https://doi.org/10.1128/AEM.00120-09	journal	June 2009
Occurrence of hopanoid lipids in anaerobic Geobacter species HÃ¤rtner, Thomas; Straub, Kristina L.; Kannenberg, Elmar FEMS Microbiology Letters, Vol. 243, Issue 1 https://doi.org/10.1016/j.femsle.2004.11.039	journal	February 2005
Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community White, Richard Allen; Chan, Amy M.; Gavelis, Gregory S. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01531	journal	January 2016
Fatty acids of bacterial origin in contemporary marine sediments Perry, G. J.; Volkman, J. K.; Johns, R. B. Geochimica et Cosmochimica Acta, Vol. 43, Issue 11 https://doi.org/10.1016/0016-7037(79)90020-6	journal	November 1979
Microfossils and possible microfossils from the early archean onverwacht group, Barberton mountain land, South Africa Walsh, Maud W. Precambrian Research, Vol. 54, Issue 2-4 https://doi.org/10.1016/0301-9268(92)90074-X	journal	January 1992
An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea McDonald, Daniel; Price, Morgan N.; Goodrich, Julia The ISME Journal, Vol. 6, Issue 3 https://doi.org/10.1038/ismej.2011.139	journal	December 2011
Microbial diversity of extant stromatolites in the hypersaline marine environment of Shark Bay, Australia Burns, Brendan P.; Goh, Falicia; Allen, Michelle Environmental Microbiology, Vol. 6, Issue 10 https://doi.org/10.1111/j.1462-2920.2004.00651.x	journal	October 2004
Hopanoid Biosynthesis and Function in Bacteria Kannenberg, Elmar L.; Poralla, Karl Naturwissenschaften, Vol. 86, Issue 4 https://doi.org/10.1007/s001140050592	journal	April 1999
Lipid biomarker and carbon isotopic signatures for stromatolite-forming, microbial mat communities and Phormidium cultures from Yellowstone National Park Jahnke, Linda L.; Embaye, Tsegereda; Hope, Janet Geobiology, Vol. 2, Issue 1 https://doi.org/10.1111/j.1472-4677.2004.00021.x	journal	January 2004
Giant Holocene Freshwater Microbialites, Laguna Bacalar, Quintana Roo, Mexico: Freshwater microbialites, Bacalar, Mexico Gischler, Eberhard; Gibson, Michael A.; Oschmann, Wolfgang Sedimentology, Vol. 55, Issue 5 https://doi.org/10.1111/j.1365-3091.2007.00946.x	journal	February 2008
Microbe-mineral interactions: early carbonate precipitation in a hypersaline lake (Eleuthera Island, Bahamas): Microbe-mineral interactions, Eleuthera Island, Bahamas Dupraz, C.; Visscher, P. T.; Baumgartner, L. K. Sedimentology, Vol. 51, Issue 4 https://doi.org/10.1111/j.1365-3091.2004.00649.x	journal	June 2004
Existence and implications of hop-17(21)-enes in the lower Cretaceous of the Saihantala Sag, Erlian Basin, China Luo, Qingyong; Yu, Shijun; Liu, Yan Petroleum Science, Vol. 9, Issue 2 https://doi.org/10.1007/s12182-012-0195-8	journal	June 2012
Growth morphologies of modern marine stromatolites: A case study from Highborne Cay, Bahamas Andres, Miriam S.; Pamela Reid, R. Sedimentary Geology, Vol. 185, Issue 3-4 https://doi.org/10.1016/j.sedgeo.2005.12.020	journal	March 2006
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample Caporaso, J. G.; Lauber, C. L.; Walters, W. A. Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1 https://doi.org/10.1073/pnas.1000080107	journal	June 2010
Aquatic ecosystems of the Yucatán Peninsula (Mexico), Belize, and Guatemala Pérez, Liseth; Bugja, Rita; Lorenschat, Julia Hydrobiologia, Vol. 661, Issue 1 https://doi.org/10.1007/s10750-010-0552-9	journal	November 2010
An abiotic model for stromatolite morphogenesis Grotzinger, John P.; Rothman, Daniel H. Nature, Vol. 383, Issue 6599 https://doi.org/10.1038/383423a0	journal	October 1996
Microbial carbonates: the geological record of calcified bacterial-algal mats and biofilms Riding, Robert Sedimentology, Vol. 47 https://doi.org/10.1046/j.1365-3091.2000.00003.x	journal	February 2000
Lipid biomarkers in Hamelin Pool microbial mats and stromatolites Allen, Michelle A.; Neilan, Brett A.; Burns, Brendan P. Organic Geochemistry, Vol. 41, Issue 11 https://doi.org/10.1016/j.orggeochem.2010.07.007	journal	November 2010
Attached and suspended microbial communities in a pristine confined aquifer: ATTACHED AND SUSPENDED MICROBIAL COMMUNITIES Flynn, Theodore M.; Sanford, Robert A.; Bethke, Craig M. Water Resources Research, Vol. 44, Issue 7 https://doi.org/10.1029/2007WR006633	journal	July 2008
Phospholipid Ester-linked Fatty Acid Biomarkers of Acetate-oxidizing Sulphate-reducers and Other Sulphide-forming Bacteria Dowling, N. J. E.; Widdel, F.; White, D. C. Microbiology, Vol. 132, Issue 7 https://doi.org/10.1099/00221287-132-7-1815	journal	July 1986
Formation of lithified micritic laminae in modern marine stromatolites (Bahamas); the role of sulfur cycling Visscher, Pieter T.; Reid, R. Pamela; Bebout, Brad M. American Mineralogist, Vol. 83, Issue 11-12 Part 2 https://doi.org/10.2138/am-1998-11-1236	journal	December 1998
Examining the global distribution of dominant archaeal populations in soil Bates, Scott T.; Berg-Lyons, Donna; Caporaso, J. Gregory The ISME Journal, Vol. 5, Issue 5 https://doi.org/10.1038/ismej.2010.171	journal	November 2010
Primer and platform effects on 16S rRNA tag sequencing Tremblay, Julien; Singh, Kanwar; Fern, Alison Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00771	journal	August 2015
Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay Goh, Falicia; Allen, Michelle A.; Leuko, Stefan The ISME Journal, Vol. 3, Issue 4 https://doi.org/10.1038/ismej.2008.114	journal	December 2008
Inner workings of thrombolites: spatial gradients of metabolic activity as revealed by metatranscriptome profiling Mobberley, J. M.; Khodadad, C. L. M.; Visscher, P. T. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep12601	journal	July 2015
Groundwater geochemistry of the Yucatan Peninsula, Mexico: Constraints on stratigraphy and hydrogeology Perry, Eugene; Paytan, Adina; Pedersen, Bianca Journal of Hydrology, Vol. 367, Issue 1-2 https://doi.org/10.1016/j.jhydrol.2008.12.026	journal	March 2009
The role of microbes in accretion, lamination and early lithification of modern marine stromatolites Reid, R. P.; Visscher, P. T.; Decho, A. W. Nature, Vol. 406, Issue 6799 https://doi.org/10.1038/35023158	journal	August 2000
Reassessing the first appearance of eukaryotes and cyanobacteria Rasmussen, Birger; Fletcher, Ian R.; Brocks, Jochen J. Nature, Vol. 455, Issue 7216 https://doi.org/10.1038/nature07381	journal	October 2008
Thermodynamic and Kinetic Response of Microbial Reactions to High CO2 Jin, Qusheng; Kirk, Matthew F. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01696	journal	November 2016
Biomarkers from Huronian oil-bearing fluid inclusions: An uncontaminated record of life before the Great Oxidation Event Dutkiewicz, Adriana; Volk, Herbert; George, Simon C. Geology, Vol. 34, Issue 6 https://doi.org/10.1130/G22360.1	journal	January 2006
Search and clustering orders of magnitude faster than BLAST Edgar, Robert C. Bioinformatics, Vol. 26, Issue 19, p. 2460-2461 https://doi.org/10.1093/bioinformatics/btq461	journal	August 2010
Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British Columbia, Canada Russell, J. A.; Brady, A. L.; Cardman, Z. Geobiology, Vol. 12, Issue 3 https://doi.org/10.1111/gbi.12082	journal	March 2014
Petroleum degradation and associated microbial signatures at the Chapopote asphalt volcano, Southern Gulf of Mexico Schubotz, Florence; Lipp, Julius S.; Elvert, Marcus Geochimica et Cosmochimica Acta, Vol. 75, Issue 16 https://doi.org/10.1016/j.gca.2011.05.025	journal	August 2011
Genomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales Carvalho, Fabiola M.; Souza, Rangel C.; Barcellos, Fernando G. BMC Microbiology, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2180-10-37	journal	January 2010
Photosynthetic isotope biosignatures in laminated micro-stromatolitic and non-laminated nodules associated with modern, freshwater microbialites in Pavilion Lake, B.C. Brady, A. L.; Slater, G. F.; Omelon, C. R. Chemical Geology, Vol. 274, Issue 1-2 https://doi.org/10.1016/j.chemgeo.2010.03.016	journal	June 2010
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies Klindworth, Anna; Pruesse, Elmar; Schweer, Timmy Nucleic Acids Research, Vol. 41, Issue 1 https://doi.org/10.1093/nar/gks808	journal	August 2012
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin Nucleic Acids Research, Vol. 41, Issue D1 https://doi.org/10.1093/nar/gks1219	journal	November 2012
Aerobic sulfate reduction in microbial mats Canfield, D.; Des Marais, D. Science, Vol. 251, Issue 5000 https://doi.org/10.1126/science.11538266	journal	March 1991
Processes of carbonate precipitation in modern microbial mats Dupraz, Christophe; Reid, R. Pamela; Braissant, Olivier Earth-Science Reviews, Vol. 96, Issue 3 https://doi.org/10.1016/j.earscirev.2008.10.005	journal	October 2009
Lipid biomarker analysis of cyanobacteria-dominated microbial mats in meltwater ponds on the McMurdo Ice Shelf, Antarctica Jungblut, Anne D.; Allen, Michelle A.; Burns, Brendan P. Organic Geochemistry, Vol. 40, Issue 2 https://doi.org/10.1016/j.orggeochem.2008.10.002	journal	February 2009
Review: The Yucatán Peninsula karst aquifer, Mexico Bauer-Gottwein, Peter; Gondwe, Bibi R. N.; Charvet, Guillaume Hydrogeology Journal, Vol. 19, Issue 3 https://doi.org/10.1007/s10040-010-0699-5	journal	January 2011
2-Methylhopanoids as biomarkers for cyanobacterial oxygenic photosynthesis Summons, Roger E.; Jahnke, Linda L.; Hope, Janet M. Nature, Vol. 400, Issue 6744 https://doi.org/10.1038/23005	journal	August 1999
Chemotaxonomy of cyanobacteria Cohen, Zvi; Margheri, Maria Cristina; Tomaselli, Luisa Phytochemistry, Vol. 40, Issue 4 https://doi.org/10.1016/0031-9422(95)00335-5	journal	November 1995
Reconnaissance isotopic and hydrochemical study of Cuatro Ciénegas groundwater, Coahuila, México Johannesson, Karen H.; Cortés, Alejandra; Kilroy, Kathryn C. Journal of South American Earth Sciences, Vol. 17, Issue 2 https://doi.org/10.1016/j.jsames.2004.01.002	journal	October 2004
Biotransformation pathways of phytol in recent anoxic sediments Grossi, V.; Hirschler, A.; Raphel, D. Organic Geochemistry, Vol. 29, Issue 4 https://doi.org/10.1016/S0146-6380(98)00118-1	journal	January 1998
The Occurrence and Effect of Sulfate Reduction and Sulfide Oxidation on Coastal Limestone Dissolution in Yucatan Cenotes Stoessell, Ronald K.; Moore, Yolanda H.; Coke, James G. Ground Water, Vol. 31, Issue 4 https://doi.org/10.1111/j.1745-6584.1993.tb00589.x	journal	July 1993
Niche differentiation of bacterial communities at a millimeter scale in Shark Bay microbial mats Wong, Hon Lun; Smith, Daniela-Lee; Visscher, Pieter T. Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep15607	journal	October 2015
Identifying Different Populations of Sulphate-reducing Bacteria within Marine Sediment Systems, Using Fatty Acid Biomarkers Taylor, J.; Parkes, R. J. Microbiology, Vol. 131, Issue 3 https://doi.org/10.1099/00221287-131-3-631	journal	March 1985
Microscale observations of sulfate reduction: Correlation of microbial activity with lithified micritic laminae in modern marine stromatolites Visscher, Pieter T.; Pamela Reid, R.; Bebout, Brad M. Geology, Vol. 28, Issue 10 https://doi.org/10.1130/0091-7613(2000)28<919:MOOSRC>2.0.CO;2	journal	January 2000
Dynamics of archaea at fine spatial scales in Shark Bay mat microbiomes Wong, Hon Lun; Visscher, Pieter T.; White III, Richard Allen Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep46160	journal	April 2017
Characterizing microbial communities and processes in a modern stromatolite (Shark Bay) using lipid biomarkers and two-dimensional distributions of porewater solutes: Microbial communities and processes in a modern stromatolite Pagès, Anais; Grice, Kliti; Vacher, Michael Environmental Microbiology, Vol. 16, Issue 8 https://doi.org/10.1111/1462-2920.12378	journal	February 2014
Hydrogen-isotopic variability in fatty acids from Yellowstone National Park hot spring microbial communities Osburn, Magdalena R.; Sessions, Alex L.; Pepe-Ranney, Charles Geochimica et Cosmochimica Acta, Vol. 75, Issue 17 https://doi.org/10.1016/j.gca.2011.05.038	journal	September 2011
Fractionation of Hydrogen Isotopes by Sulfate- and Nitrate-Reducing Bacteria Osburn, Magdalena R.; Dawson, Katherine S.; Fogel, Marilyn L. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01166	journal	August 2016
Formation of phytadienes in the water column: myth or reality? Grossi, V.; Baas, M.; Schogt, N. Organic Geochemistry, Vol. 24, Issue 8-9 https://doi.org/10.1016/S0146-6380(96)00071-X	journal	August 1996
QIIME allows analysis of high-throughput community sequencing data Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse Nature Methods, Vol. 7, Issue 5 https://doi.org/10.1038/nmeth.f.303	journal	April 2010
Distribution of Hopanoid Triterpenes in Prokaryotes Rohmer, M.; Bouvier-Nave, P.; Ourisson, G. Microbiology, Vol. 130, Issue 5 https://doi.org/10.1099/00221287-130-5-1137	journal	May 1984
Stromatolite reef from the Early Archaean era of Australia Allwood, Abigail C.; Walter, Malcolm R.; Kamber, Balz S. Nature, Vol. 441, Issue 7094 https://doi.org/10.1038/nature04764	journal	June 2006
Lake Thetis, Western Australia: and example of Saline Lake Sedimentation dominated by Benthic Microbial Processes Grey, K.; Moore, Ls; Burne, Rv Marine and Freshwater Research, Vol. 41, Issue 2 https://doi.org/10.1071/MF9900275	journal	January 1990
The Cellular Fatty Acids of the Sulphate-reducing Bacteria, Desulfobacter sp., Desulfobulbus sp. and Desulfovibrio desulfuricans Taylor, J.; Parkes, R. J. Microbiology, Vol. 129, Issue 11 https://doi.org/10.1099/00221287-129-11-3303	journal	November 1983
DNA extraction from benthic Cyanobacteria: comparative assessment and optimization Gaget, V.; Keulen, A.; Lau, M. Journal of Applied Microbiology, Vol. 122, Issue 1 https://doi.org/10.1111/jam.13332	journal	December 2016
Cyanobacterial construction of hot spring siliceous stromatolites in Yellowstone National Park: Cyanobacterial diversity of YNP stromatolites Pepe-Ranney, Charles; Berelson, William M.; Corsetti, Frank A. Environmental Microbiology, Vol. 14, Issue 5 https://doi.org/10.1111/j.1462-2920.2012.02698.x	journal	February 2012
Identification of a methylase required for 2-methylhopanoid production and implications for the interpretation of sedimentary hopanes Welander, P. V.; Coleman, M. L.; Sessions, A. L. Proceedings of the National Academy of Sciences, Vol. 107, Issue 19 https://doi.org/10.1073/pnas.0912949107	journal	April 2010
PEAR: a fast and accurate Illumina Paired-End reAd mergeR Zhang, J.; Kobert, K.; Flouri, T. Bioinformatics, Vol. 30, Issue 5 https://doi.org/10.1093/bioinformatics/btt593	journal	October 2013
Metagenomic and stable isotopic analyses of modern freshwater microbialites in Cuatro Ciénegas, Mexico Breitbart, Mya; Hoare, Ana; Nitti, Anthony Environmental Microbiology, Vol. 11, Issue 1 https://doi.org/10.1111/j.1462-2920.2008.01725.x	journal	January 2009
Spatially Resolved Genomic, Stable Isotopic, and Lipid Analyses of a Modern Freshwater Microbialite from Cuatro Ciénegas, Mexico Nitti, Anthony; Daniels, Camille A.; Siefert, Janet Astrobiology, Vol. 12, Issue 7 https://doi.org/10.1089/ast.2011.0812	journal	July 2012
Textural and Geochemical Features of Freshwater Microbialites from Laguna Bacalar, Quintana roo, Mexico Castro-Contreras, S. I.; Gingras, M. K.; Pecoits, E. PALAIOS, Vol. 29, Issue 5 https://doi.org/10.2110/palo.2013.063	journal	May 2014
Decoupled fresh–saline groundwater circulation of a coastal carbonate aquifer: Spatial patterns of temperature and specific electrical conductivity Beddows, Patricia A.; Smart, Peter L.; Whitaker, Fiona F. Journal of Hydrology, Vol. 346, Issue 1-2 https://doi.org/10.1016/j.jhydrol.2007.08.013	journal	November 2007
A Rapid Method of Total Lipid Extraction and Purification Bligh, E. G.; Dyer, W. J. Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1 https://doi.org/10.1139/y59-099	journal	January 1959
The Hydrogeochemistry of the Karst Aquifer System of the Northern Yucatan Peninsula, Mexico Perry, Eugene; Velazquez-Oliman, Guadalupe; Marin, Luis International Geology Review, Vol. 44, Issue 3 https://doi.org/10.2747/0020-6814.44.3.191	journal	March 2002
Essential polyunsaturated fatty acids from 14 species of diatom (Bacillariophyceae) Dunstan, Graeme A.; Volkman, John K.; Barrett, Stephanie M. Phytochemistry, Vol. 35, Issue 1 https://doi.org/10.1016/S0031-9422(00)90525-9	journal	December 1993
Sulfate reducing bacteria in microbial mats: Changing paradigms, new discoveries Baumgartner, L. K.; Reid, R. P.; Dupraz, C. Sedimentary Geology, Vol. 185, Issue 3-4 https://doi.org/10.1016/j.sedgeo.2005.12.008	journal	March 2006
The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes Meyer, F.; Paarmann, D.; D'Souza, M. BMC Bioinformatics, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2105-9-386	journal	September 2008
Fern constituents: pentacyclic triterpenoids isolated from Polypodium niponicum and P. formosanum Ageta, Hiroyuki; Arai, Yoko Phytochemistry, Vol. 22, Issue 8 https://doi.org/10.1016/S0031-9422(00)80275-7	journal	January 1983
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A. The ISME Journal, Vol. 6, Issue 8 https://doi.org/10.1038/ismej.2012.8	journal	March 2012
Comparative analysis of extractable lipids in hot spring microbial mats and their component photosynthetic bacteria Shiea, Jentaie; Brassel, Simon C.; Ward, David M. Organic Geochemistry, Vol. 17, Issue 3 https://doi.org/10.1016/0146-6380(91)90094-Z	journal	January 1991
Geological and trace element evidence for a marine sedimentary environment of deposition and biogenicity of 3.45 Ga stromatolitic carbonates in the Pilbara Craton, and support for a reducing Archaean ocean Van Kranendonk, Martin J.; Webb, Gregory E.; Kamber, Balz S. Geobiology, Vol. 1, Issue 2 https://doi.org/10.1046/j.1472-4669.2003.00014.x	journal	October 2003
Stromatolitic knobs in Storr's Lake (San Salvador, Bahamas): a model system for formation and alteration of laminae Dupraz, C.; Fowler, A.; Tobias, C. Geobiology, Vol. 11, Issue 6 https://doi.org/10.1111/gbi.12063	journal	October 2013
Bacterially mediated precipitation in marine stromatolites Paerl, Hans W.; Steppe, Timothy F.; Reid, R. Pamela Environmental Microbiology, Vol. 3, Issue 2 https://doi.org/10.1046/j.1462-2920.2001.00168.x	journal	February 2001
Microbial biomass: A catalyst for CaCO3 precipitation in advection-dominated transport regimes Kandianis, M. T.; Fouke, B. W.; Johnson, R. W. Geological Society of America Bulletin, Vol. 120, Issue 3-4 https://doi.org/10.1130/B26188.1	journal	March 2008
Autotrophic and heterotrophic associated biosignatures in modern freshwater microbialites over seasonal and spatial gradients Brady, Allyson L.; Laval, Bernard; Lim, Darlene S. S. Organic Geochemistry, Vol. 67 https://doi.org/10.1016/j.orggeochem.2013.11.013	journal	February 2014
Unravelling core microbial metabolisms in the hypersaline microbial mats of Shark Bay using high-throughput metagenomics Ruvindy, Rendy; White III, Richard Allen; Neilan, Brett Anthony The ISME Journal, Vol. 10, Issue 1 https://doi.org/10.1038/ismej.2015.87	journal	May 2015
Diversity of Sulfate-Reducing Bacteria in Oxic and Anoxic Regions of a Microbial Mat Characterized by Comparative Analysis of Dissimilatory Sulfite Reductase Genes Minz, Dror; Flax, Jodi L.; Green, Stefan J. Applied and Environmental Microbiology, Vol. 65, Issue 10 https://doi.org/10.1128/AEM.65.10.4666-4671.1999	journal	October 1999
Microscale observations of sulfate reduction: Correlation of microbial activity with lithified micritic laminae in modern marine stromatolites Visscher, Pieter T.; Pamela Reid, R.; Bebout, Brad M. Geology, Vol. 28, Issue 10 https://doi.org/10.1130/0091-7613(2000)028<0919:moosrc>2.3.co;2	journal	October 2000
A Rapid Method of Total Lipid Extraction and Purification Bligh, E. G.; Dyer, W. J. Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8 https://doi.org/10.1139/o59-099	journal	August 1959
Formation of lithified micritic laminae in modern marine stromatolites (Bahamas); the role of sulfur cycling Visscher, Pieter T.; Reid, R. Pamela; Bebout, Brad M. American Mineralogist, Vol. 83, Issue 11-12 Part 2 https://doi.org/10.2138/am-1997-11-1236	journal	December 1998
Analysis of immune, microbiota and metabolome maturation in infants in a clinical trial of Lactobacillus paracasei CBA L74-fermented formula Roggero, Paola; Liotto, Nadia; Pozzi, Chiara Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16582-1	journal	June 2020
Primer and platform effects on 16S rRNA tag sequencing Alison, Fern,; Feng, Chen,; Julien, Tremblay, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/wgfs-e050	text	January 2015

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