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Title: Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles

Abstract

Biomimetic systems responsive to environmental stimuli are of growing interest due to their useful, intriguing, and sometimes unexpected properties. Hierarchical self-assembly of biological building blocks has emerged as a powerful means of creating biomaterials with collective properties. Here, we show P22 virus-like particles (VLPs) functionalized with a spider silk protein derivative on the capsid exterior assemble into a hierarchical structure due to spider silk/spider silk interactions at low pH and reversibly dissemble upon raising the pH. We also show that the capsid arrays can be assembled through electrostatic interaction between the negatively charged capsids and a positively supercharged GFP mutant and can be reversed by raising the ionic strength. Most notably, we found that the supercharged GFP could bind to the hierarchically assembled material under high salt conditions but not to the individual capsids under the same high salt conditions. As a result, the binding of the charged macromolecule under high salt conditions demonstrates a collective behavior of the hierarchically assembled system that is not present with the unassembled, individual components of the system.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1];  [2]; ORCiD logo [3]; ORCiD logo [1]
  1. Indiana Univ., Bloomington, IN (United States)
  2. Montana State Univ., Bozeman, MT (United States)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1461344
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Chemistry of Materials
Additional Journal Information:
Journal Volume: 30; Journal Issue: 7; Journal ID: ISSN 0897-4756
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Aumiller, Jr., William M., Uchida, Masaki, Biner, Daniel W., Miettinen, Heini M., Lee, Byeongdu, and Douglas, Trevor. Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles. United States: N. p., 2018. Web. doi:10.1021/acs.chemmater.7b04964.
Aumiller, Jr., William M., Uchida, Masaki, Biner, Daniel W., Miettinen, Heini M., Lee, Byeongdu, & Douglas, Trevor. Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles. United States. https://doi.org/10.1021/acs.chemmater.7b04964
Aumiller, Jr., William M., Uchida, Masaki, Biner, Daniel W., Miettinen, Heini M., Lee, Byeongdu, and Douglas, Trevor. 2018. "Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles". United States. https://doi.org/10.1021/acs.chemmater.7b04964. https://www.osti.gov/servlets/purl/1461344.
@article{osti_1461344,
title = {Stimuli Responsive Hierarchical Assembly of P22 Virus-like Particles},
author = {Aumiller, Jr., William M. and Uchida, Masaki and Biner, Daniel W. and Miettinen, Heini M. and Lee, Byeongdu and Douglas, Trevor},
abstractNote = {Biomimetic systems responsive to environmental stimuli are of growing interest due to their useful, intriguing, and sometimes unexpected properties. Hierarchical self-assembly of biological building blocks has emerged as a powerful means of creating biomaterials with collective properties. Here, we show P22 virus-like particles (VLPs) functionalized with a spider silk protein derivative on the capsid exterior assemble into a hierarchical structure due to spider silk/spider silk interactions at low pH and reversibly dissemble upon raising the pH. We also show that the capsid arrays can be assembled through electrostatic interaction between the negatively charged capsids and a positively supercharged GFP mutant and can be reversed by raising the ionic strength. Most notably, we found that the supercharged GFP could bind to the hierarchically assembled material under high salt conditions but not to the individual capsids under the same high salt conditions. As a result, the binding of the charged macromolecule under high salt conditions demonstrates a collective behavior of the hierarchically assembled system that is not present with the unassembled, individual components of the system.},
doi = {10.1021/acs.chemmater.7b04964},
url = {https://www.osti.gov/biblio/1461344}, journal = {Chemistry of Materials},
issn = {0897-4756},
number = 7,
volume = 30,
place = {United States},
year = {Thu Mar 15 00:00:00 EDT 2018},
month = {Thu Mar 15 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 12 works
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Works referenced in this record:

DNA-mediated engineering of multicomponent enzyme crystals
journal, March 2015


A general strategy for the DNA-mediated self-assembly of functional nanoparticles into heterogeneous systems
journal, October 2013


Structural diversity in binary nanoparticle superlattices
journal, January 2006


Natural supramolecular building blocks: from virus coat proteins to viral nanoparticles
journal, January 2012


Assembly, stability and dynamics of virus capsids
journal, March 2013


Viruses: Making Friends with Old Foes
journal, May 2006


Combining Protein Cages and Polymers: from Understanding Self-Assembly to Functional Materials
journal, August 2016


Dual-Surface Modified Virus Capsids for Targeted Delivery of Photodynamic Agents to Cancer Cells
journal, September 2010


Directed Evolution of a Protein Container
journal, February 2011


Genetically Programmed In Vivo Packaging of Protein Cargo and Its Controlled Release from Bacteriophage P22
journal, June 2011


Self-assembling biomolecular catalysts for hydrogen production
journal, December 2015


Electrostatic assembly of binary nanoparticle superlattices using protein cages
journal, December 2012


Electrostatic Self-Assembly of Soft Matter Nanoparticle Cocrystals with Tunable Lattice Parameters
journal, October 2015


DNA-controlled assembly of a NaTl lattice structure from gold nanoparticles and protein nanoparticles
journal, October 2010


Emerging applications of stimuli-responsive polymer materials
journal, January 2010


CO 2 -Responsive polymer materials
journal, January 2017


pH-Responsive polymers
journal, January 2017


Hydrogels in a historical perspective: From simple networks to smart materials
journal, September 2014


Self-assembly and optically triggered disassembly of hierarchical dendron–virus complexes
journal, March 2010


Stimuli-responsive polymers and their applications
journal, January 2017


pH-Responsive polymers: synthesis, properties and applications
journal, January 2008


The Concept of Integrative Levels and Biology
journal, March 1945


The concept of self-organization in cellular architecture
journal, October 2001


Collective motion
journal, August 2012


Regular pattern formation in real ecosystems
journal, March 2008


Coordination and collective properties of molecular motors: theory
journal, February 2010


Bacterial biofilms: from the Natural environment to infectious diseases
journal, February 2004


What drives bacteria to produce a biofilm?
journal, July 2004


Biofilms: an emergent form of bacterial life
journal, August 2016


Mechanisms of Virus Assembly
journal, April 2015


Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM
journal, December 2015


Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation
journal, February 2014


Self-assembly of spider silk proteins is controlled by a pH-sensitive relay
journal, May 2010


Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
journal, October 2014


Higher Order Assembly of Virus-like Particles (VLPs) Mediated by Multi-valent Protein Linkers
journal, January 2015


Small Angle X-ray Scattering for Nanoparticle Research
journal, April 2016


Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis
journal, November 2017


Self-Assembly Mechanism for a Naphthalene−Dipeptide Leading to Hydrogelation
journal, November 2009


pH‐Dependent Dimerization of Spider Silk N-Terminal Domain Requires Relocation of a Wedged Tryptophan Side Chain
journal, September 2012


Toward spinning artificial spider silk
journal, April 2015


Modular interior loading and exterior decoration of a virus-like particle
journal, January 2017


Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System
journal, January 2016


Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle
journal, November 2013


Coconfinement of Fluorescent Proteins: Spatially Enforced Communication of GFP and mCherry Encapsulated within the P22 Capsid
journal, November 2012


Works referencing / citing this record:

Supramolecular step-growth polymerization kinetics of pre-assembled triblock copolymer micelles
journal, January 2019


A review on virus protein self-assembly
journal, November 2019


Functional protein shells fabricated from the self-assembling protein sheets of prokaryotic organelles
journal, January 2020


Bioengineering Strategies for Protein-Based Nanoparticles
journal, July 2018


Virus-derived materials: bury the hatchet with old foes
journal, January 2020