A sorghum NAC gene is associated with variation in biomass properties and yield potential

Xia, Jingnu; Zhao, Yunjun; Burks, Payne; Pauly, Markus; Brown, Patrick J.

doi:10.1002/pld3.70

Title: A sorghum NAC gene is associated with variation in biomass properties and yield potential

Journal Article · Mon Jul 23 00:00:00 EDT 2018 · Plant Direct

DOI:https://doi.org/10.1002/pld3.70· OSTI ID:1461217

Xia, Jingnu ^[1]; Zhao, Yunjun ^[2]; Burks, Payne ^[1]; Pauly, Markus ^[2]; Brown, Patrick J. ^[1]

Department of Crop Sciences University of Illinois at Urbana Champaign Urbana Illinois
Department of Plant and Microbial Biology University of California, Berkeley Berkeley California

Sorghum bicolor is a C₄ grass widely cultivated for grain, forage, sugar, and biomass. The sorghum Dry Stalk (D) locus controls a qualitative difference between juicy green (dd) and dry white (D-) stalks and midribs, and co-localizes with a quantitative trait locus for sugar yield. Here, we apply fine-mapping and genome-wide association study (GWAS) to identify a candidate gene underlying D, and use nearly isogenic lines (NILs) to characterize the transcriptional, compositional, and agronomic effects of variation at the D locus. The D locus was fine-mapped to a 36 kb interval containing four genes. One of these genes is a NAC transcription factor that contains a stop codon in the NAC domain in the recessive (dd) parent. Allelic variation at D affects grain yield, sugar yield, and biomass composition in NILs. Green midrib (dd) NILs show reductions in lignin in stalk tissue and produce higher sugar and grain yields under well-watered field conditions. Increased yield potential in dd NILs is associated with increased stalk mass and moisture, higher biomass digestibility, and an extended period of grain filling. Transcriptome profiling of midrib tissue at the 4–6 leaf stages, when NILs first become phenotypically distinct, reveals that dd NILs have increased expression of a miniature zinc finger (MIF) gene. MIF genes dimerize with and suppress zinc finger homeodomain (ZF-HD) transcription factors, and a ZF-HD gene is associated with midrib color variation in a GWAS analysis across 1,694 diverse sorghum inbreds. A premature stop codon in a NAC gene is the most likely candidate polymorphism underlying the sorghum D locus. More detailed understanding of the sorghum D locus could help improve agronomic potential in cereals.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: DE‐SC0012400; SC0012400

OSTI ID:: 1461217

Alternate ID(s):: OSTI ID: 1461218; OSTI ID: 1575207; OSTI ID: 1575208

Journal Information:: Plant Direct, Journal Name: Plant Direct Vol. 2 Journal Issue: 7; ISSN 2475-4455

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 25 works

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Title: A sorghum NAC gene is associated with variation in biomass properties and yield potential

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