Production of oil in vegetative tissues

Benning, Christoph; Sanjaya, Sedbrook J.; Changcheng, Xu

Production of oil in vegetative tissues

Patent · 26 June 2018

OSTI ID:1460544

Benning, Christoph; Sanjaya, Sedbrook J.; Changcheng, Xu

The present invention relates to compositions and methods for providing RNA interference (RNAi) vectors comprising trigalactosyldiacylglycerol (tgd) biosynthesis enzyme constructs for increasing oil content of plants. Further, the use of tgd RNAi silencing vectors in combination with co-expression of heterologous oil regulating transcription factors, such as WRINKLED1, are contemplated to overcome the reduced growth and variable levels of embryonic lethality in plants with reduced TGD protein. Additionally, plants having reduced APS1, a gene encoding a major catalytic isoform of the small subunit of AGPase (AGP(-) plants), co-expressing a heterologous oil modulating transcription factor are contemplated for use in combination with plants having reduced TGD. Oil harvested from vegetative tissues of plants comprising vectors and genes of the present invention is contemplated for use in biofuel and biodiesel products.

Research Organization:: Board of Trustees of Michigan State University, East Lansing, MI (United States)

Sponsoring Organization:: USDOE

Assignee:: Board of Trustees of Michigan State University (East Lansing, MI)

Patent Number(s):: 10,006,039

Application Number:: 14/046,504

OSTI ID:: 1460544

Country of Publication:: United States

Language:: English

References (10)

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WRI1 Is Required for Seed Germination and Seedling Establishment Cernac, Alex; Andre, Carl; Hoffmann-Benning, Susanne Plant Physiology, Vol. 141, Issue 2, p. 745-757 https://doi.org/10.1104/pp.106.079574	journal	June 2006
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Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli Hill, Margaret A.; Preiss, Jack Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577 https://doi.org/10.1006/bbrc.1998.8301	journal	March 1998
Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis No authors listed Plant Biotechnology Journal, Vol. 9, Issue 8, p. 874-883 https://doi.org/10.1111/j.1467-7652.2011.00599.x	journal	February 2011
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Production of oil in vegetative tissues

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