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Title: Influence of isopropylmalate synthase Os IPMS 1 on seed vigour associated with amino acid and energy metabolism in rice

Abstract

Summary Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase ( IPMS ) catalyses the committed step of leucine (Leu) biosynthesis, but its effect on seed vigour remains unclear. In this study, rice Os IPMS 1 and Os IPMS 2 was cloned, and the roles of Os IPMS 1 in seed vigour were mainly investigated. Os IPMS 1 and Os IPMS 2 catalyse Leu biosynthesis, and Leu feedback inhibits their IPMS activities. Disruption of Os IPMS 1 resulted in low seed vigour under various conditions, which might be tightly associated with the reduction of amino acids in germinating seeds. Eleven amino acids that associated with stress tolerance, GA biosynthesis and tricarboxylic acid ( TCA ) cycle were significantly reduced in osipms1 mutants compared with those in wide type ( WT ) during seed germination. Transcriptome analysis indicated that a total of 1209 differentially expressed genes ( DEG s) were altered in osipms1a mutant compared with WT at the early germination stage, wherein most of the genes were involved in glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolism. Further analysis confirmed that the regulation of Os IPMS 1 in seed vigour involved in starch hydrolysis,more » glycolytic activity and energy levels in germinating seeds. The effects of seed priming were tightly associated with the mRNA levels of Os IPMS 1 in priming seeds. The Os IPMS 1 might be used as a biomarker to determine the best stop time‐point of seed priming in rice. This study provides novel insights into the function of Os IPMS 1 on seed vigour and should have practical applications in seed priming of rice.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [2]
  1. The Laboratory of Seed Science and Technology State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural University Nanjing China
  2. The Laboratory of Seed Science and Technology State Key Laboratory of Crop Genetics and Germplasm Enhancement Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural University Nanjing China, The Laboratory of Seed Science and Technology Guangdong Key Laboratory of Plant Molecular Breeding State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources South China Agricultural University Guangzhou China
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1460467
Alternate Identifier(s):
OSTI ID: 1460468
Resource Type:
Journal Article: Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Name: Plant Biotechnology Journal Journal Volume: 17 Journal Issue: 2; Journal ID: ISSN 1467-7644
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

He, Yongqi, Cheng, Jinping, He, Ying, Yang, Bin, Cheng, Yanhao, Yang, Can, Zhang, Hongsheng, and Wang, Zhoufei. Influence of isopropylmalate synthase Os IPMS 1 on seed vigour associated with amino acid and energy metabolism in rice. United Kingdom: N. p., 2018. Web. doi:10.1111/pbi.12979.
He, Yongqi, Cheng, Jinping, He, Ying, Yang, Bin, Cheng, Yanhao, Yang, Can, Zhang, Hongsheng, & Wang, Zhoufei. Influence of isopropylmalate synthase Os IPMS 1 on seed vigour associated with amino acid and energy metabolism in rice. United Kingdom. https://doi.org/10.1111/pbi.12979
He, Yongqi, Cheng, Jinping, He, Ying, Yang, Bin, Cheng, Yanhao, Yang, Can, Zhang, Hongsheng, and Wang, Zhoufei. 2018. "Influence of isopropylmalate synthase Os IPMS 1 on seed vigour associated with amino acid and energy metabolism in rice". United Kingdom. https://doi.org/10.1111/pbi.12979.
@article{osti_1460467,
title = {Influence of isopropylmalate synthase Os IPMS 1 on seed vigour associated with amino acid and energy metabolism in rice},
author = {He, Yongqi and Cheng, Jinping and He, Ying and Yang, Bin and Cheng, Yanhao and Yang, Can and Zhang, Hongsheng and Wang, Zhoufei},
abstractNote = {Summary Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase ( IPMS ) catalyses the committed step of leucine (Leu) biosynthesis, but its effect on seed vigour remains unclear. In this study, rice Os IPMS 1 and Os IPMS 2 was cloned, and the roles of Os IPMS 1 in seed vigour were mainly investigated. Os IPMS 1 and Os IPMS 2 catalyse Leu biosynthesis, and Leu feedback inhibits their IPMS activities. Disruption of Os IPMS 1 resulted in low seed vigour under various conditions, which might be tightly associated with the reduction of amino acids in germinating seeds. Eleven amino acids that associated with stress tolerance, GA biosynthesis and tricarboxylic acid ( TCA ) cycle were significantly reduced in osipms1 mutants compared with those in wide type ( WT ) during seed germination. Transcriptome analysis indicated that a total of 1209 differentially expressed genes ( DEG s) were altered in osipms1a mutant compared with WT at the early germination stage, wherein most of the genes were involved in glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolism. Further analysis confirmed that the regulation of Os IPMS 1 in seed vigour involved in starch hydrolysis, glycolytic activity and energy levels in germinating seeds. The effects of seed priming were tightly associated with the mRNA levels of Os IPMS 1 in priming seeds. The Os IPMS 1 might be used as a biomarker to determine the best stop time‐point of seed priming in rice. This study provides novel insights into the function of Os IPMS 1 on seed vigour and should have practical applications in seed priming of rice.},
doi = {10.1111/pbi.12979},
url = {https://www.osti.gov/biblio/1460467}, journal = {Plant Biotechnology Journal},
issn = {1467-7644},
number = 2,
volume = 17,
place = {United Kingdom},
year = {Mon Jul 16 00:00:00 EDT 2018},
month = {Mon Jul 16 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1111/pbi.12979

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Cited by: 45 works
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