Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Wang, Jack P.; Matthews, Megan L.; Williams, Cranos M.; Shi, Rui; Yang, Chenmin; Tunlaya-Anukit, Sermsawat; Chen, Hsi-Chuan; Li, Quanzi; Liu, Jie; Lin, Chien-Yuan; Naik, Punith; Sun, Ying-Hsuan; Loziuk, Philip L.; Yeh, Ting-Feng; Kim, Hoon; Gjersing, Erica; Shollenberger, Todd; Shuford, Christopher M.; Song, Jina; Miller, Zachary; Huang, Yung-Yun; Edmunds, Charles W.; Liu, Baoguang; Sun, Yi; Lin, Ying-Chung Jimmy; Li, Wei; Chen, Hao; Peszlen, Ilona; Ducoste, Joel J.; Ralph, John; Chang, Hou-Min; Muddiman, David C.; Davis, Mark F.; Smith, Chris; Isik, Fikret; Sederoff, Ronald; Chiang, Vincent L.

doi:10.1038/s41467-018-03863-z

Title: Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Journal Article · Fri Apr 20 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-03863-z· OSTI ID:1435499

Wang, Jack P. ^[1]; Matthews, Megan L. ^[2]; Williams, Cranos M. ^[2]; Shi, Rui ^[3]; Yang, Chenmin ^[3]; Tunlaya-Anukit, Sermsawat ^[3]; Chen, Hsi-Chuan ^[3]; Li, Quanzi ^[4]; Liu, Jie ^[3]; Lin, Chien-Yuan ^[5]; Naik, Punith ^[6]; Sun, Ying-Hsuan ^[7]; Loziuk, Philip L. ^[8]; Yeh, Ting-Feng ^[9];

^[10]; Gjersing, Erica ^[11]; Shollenberger, Todd ^[11]; Shuford, Christopher M. ^[8];

^[2]; Miller, Zachary ^[12] more »

Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
North Carolina State Univ., Raleigh, NC (United States). Electrical and Computer Engineering
North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
Chinese Academy of Forestry, Beijing (China). State Key Lab. of Tree Genetics and Breeding
North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
North Carolina State Univ., Raleigh, NC (United States). Civil, Construction and Environmental Engineering
National Chung-Hsing Univ., Taichung (Taiwan). Dept. of Forestry
North Carolina State Univ., Raleigh, NC (United States). W.M. Keck Fourier Transform Mass Spectrometry Lab., Dept. of Chemistry
National Taiwan Univ., Taipei (Taiwan). School of Forestry and Resource Conservation
Univ. of Wisconsin, Madison, WI (United States). DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Inst., Dept. of Biochemistry
National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
North Carolina State Univ., Raleigh, NC (United States). Dept. of Forest Biomaterials
North Carolina State Univ., Raleigh, NC (United States). Dept. of Operations Research
Beihua Univ., Jilin (China). Dept. of Forestry
Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding
Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; National Taiwan Univ., Taipei (Taiwan). College of Life Science, Dept. of Life Sciences
Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
North Carolina State Univ., Raleigh, NC (United States). Bioinformatics Research Center
North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources
Northeast Forestry Univ., Harbin (China). State Key Lab. of Tree Genetics and Breeding; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forestry and Environmental Resources, Forest Biotechnology Group; North Carolina State Univ., Raleigh, NC (United States). Dept. of Forest Biomaterials

A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels. Lignin is polymerized from three monomers (monolignols) produced by a grid-like pathway. The pathway in wood formation of Populus trichocarpa has at least 21 genes, encoding enzymes that mediate 37 reactions on 24 metabolites, leading to lignin and affecting wood properties. We perturb these 21 pathway genes and integrate transcriptomic, proteomic, fluxomic and phenomic data from 221 lines selected from ~2000 transgenics (6-month-old). The integrative analysis estimates how changing expression of pathway gene or gene combination affects protein abundance, metabolic-flux, metabolite concentrations, and 25 wood traits, including lignin, tree-growth, density, strength, and saccharification. The analysis then predicts improvements in any of these 25 traits individually or in combinations, through engineering expression of specific monolignol genes. The analysis may lead to greater understanding of other pathways for improved growth and adaptation.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Office of Biological and Environmental Research; National Natural Science Foundation of China (NSFC); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; 31430093; 31470672; 31522014; DBI-0922391; FC02-07ER64494; AC02-05CH11231

OSTI ID:: 1435499

Alternate ID(s):: OSTI ID: 1460355

Report Number(s):: NREL/JA-2700-71418

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 72 works

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