Structural visualization of RNA polymerase III transcription machineries
Abstract
Abstract RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III–TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and Pol III, which stabilizes the winged-helix domains of the C34 subunit of Pol III over the active site cleft. The architecture of Pol III PIC more resembles that of the Pol II PIC than the Pol I PIC. In addition, we also obtain a 3D reconstruction of Pol III in complex with TFIIIB using the elongation complex (EC) scaffold, shedding light on the mechanism of facilitated recycling of Pol III prior to transcription re-initiation.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1459999
- Alternate Identifier(s):
- OSTI ID: 1543739
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Journal Article: Published Article
- Journal Name:
- Cell Discovery
- Additional Journal Information:
- Journal Name: Cell Discovery Journal Volume: 4 Journal Issue: 1; Journal ID: ISSN 2056-5968
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Cell Biology
Citation Formats
Han, Yan, Yan, Chunli, Fishbain, Susan, Ivanov, Ivaylo, and He, Yuan. Structural visualization of RNA polymerase III transcription machineries. United Kingdom: N. p., 2018.
Web. doi:10.1038/s41421-018-0044-z.
Han, Yan, Yan, Chunli, Fishbain, Susan, Ivanov, Ivaylo, & He, Yuan. Structural visualization of RNA polymerase III transcription machineries. United Kingdom. https://doi.org/10.1038/s41421-018-0044-z
Han, Yan, Yan, Chunli, Fishbain, Susan, Ivanov, Ivaylo, and He, Yuan. 2018.
"Structural visualization of RNA polymerase III transcription machineries". United Kingdom. https://doi.org/10.1038/s41421-018-0044-z.
@article{osti_1459999,
title = {Structural visualization of RNA polymerase III transcription machineries},
author = {Han, Yan and Yan, Chunli and Fishbain, Susan and Ivanov, Ivaylo and He, Yuan},
abstractNote = {Abstract RNA polymerase III (Pol III) transcription initiation requires the action of the transcription factor IIIB (TFIIIB) and is highly regulated. Here, we determine the structures of Pol III pre-initiation complexes (PICs) using single particle cryo-electron microscopy (cryo-EM). We observe stable Pol III–TFIIIB complexes using nucleic acid scaffolds mimicking various functional states, in which TFIIIB tightly encircles the upstream promoter DNA. There is an intricate interaction between TFIIIB and Pol III, which stabilizes the winged-helix domains of the C34 subunit of Pol III over the active site cleft. The architecture of Pol III PIC more resembles that of the Pol II PIC than the Pol I PIC. In addition, we also obtain a 3D reconstruction of Pol III in complex with TFIIIB using the elongation complex (EC) scaffold, shedding light on the mechanism of facilitated recycling of Pol III prior to transcription re-initiation.},
doi = {10.1038/s41421-018-0044-z},
url = {https://www.osti.gov/biblio/1459999},
journal = {Cell Discovery},
issn = {2056-5968},
number = 1,
volume = 4,
place = {United Kingdom},
year = {Tue Jul 31 00:00:00 EDT 2018},
month = {Tue Jul 31 00:00:00 EDT 2018}
}
Web of Science
Figures / Tables:
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