Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity

Hill, Jr, Joseph Lee; Hill, Ashley Nicole; Roberts, Alison W.; Haigler, Candace H.; Tien, Ming

doi:10.1002/pld3.61

Title: Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity

Journal Article · Tue Jul 10 00:00:00 EDT 2018 · Plant Direct

DOI:https://doi.org/10.1002/pld3.61· OSTI ID:1459680

Hill, Jr, Joseph Lee ^[1]; Hill, Ashley Nicole ^[1]; Roberts, Alison W. ^[2]; Haigler, Candace H. ^[3]; Tien, Ming ^[1]

Department of Biochemistry and Molecular Biology, The Center for Lignocellulose Structure and Formation, Pennsylvania State University, University Park Pennsylvania
Department of Biological Sciences, University of Rhode Island, Kingston Rhode Island
Department of Crop and Soil Sciences and Department of Plant and Microbial Biology, North Carolina State University, Raleigh North Carolina

Here, cellulose microfibrils are synthesized by membrane-embedded cellulose synthesis complexes (CSCs), currently modeled as hexamers of cellulose synthase (CESA) trimers. The three paralogous CESAs involved in secondary cell wall (SCW) cellulose biosynthesis in Arabidopsis (CESA4, CESA7, CESA8) are similar, but nonredundant, with all three isoforms required for assembly and function of the CSC. The molecular basis of protein–protein recognition among the isoforms is not well understood. To investigate the locations of the interfaces that are responsible for isoform recognition, we swapped three domains between the Arabidopsis CESAs required for SCW synthesis (CESA4, CESA7, and CESA8): N-terminus, central domain containing the catalytic core, and C-terminus. Chimeric genes with all pairwise permutations of the domains were tested for in vivo functionality within knockout mutant backgrounds of cesa4, cesa7, and cesa8. Immunoblotting with isoform-specific antibodies confirmed the anticipated protein expression in transgenic plants. The percent recovery of stem height and crystalline cellulose content was assayed, as compared to wild type, the mutant background lines, and other controls. Retention of the native central domain was sufficient for CESA8 chimeras to function, with neither its N-terminal nor C-terminal domains required. The C-terminal domain is required for class-specific function of CESA4 and CESA7, and CESA7 also requires its own N-terminus. Across all isoforms, the results indicate that the central domain, as well as the N- and C-terminal regions, contributes to class-specific function variously in Arabidopsis CESA4, CESA7, and CESA8.

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Research Organization:: Energy Frontier Research Centers (EFRC), Washington, D.C. (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States); North Carolina State University, Raleigh, NC (United States); Univ. of Rhode Island, Kingston, RI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1459680

Alternate ID(s):: OSTI ID: 1459681; OSTI ID: 1470661; OSTI ID: 1617033

Journal Information:: Plant Direct, Journal Name: Plant Direct Vol. 2 Journal Issue: 7; ISSN 2475-4455

Publisher:: Wiley and American Society of Plant Biologists and Society for Experimental BiologyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 8 works

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Title: Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity

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