Advances and prospects in metabolic engineering of Zymomonas mobilis

Wang, Xia; He, Qiaoning; Yang, Yongfu; Wang, Jingwen; Haning, Katie; Hu, Yun; Wu, Bo; He, Mingxiong; Zhang, Yaoping; Bao, Jie; Contreras, Lydia M.; Yang, Shihui

doi:10.1016/j.ymben.2018.04.001

Title: Advances and prospects in metabolic engineering of Zymomonas mobilis

Journal Article · Thu Apr 05 00:00:00 EDT 2018 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2018.04.001· OSTI ID:1459643

Wang, Xia ^[1]; He, Qiaoning ^[1]; Yang, Yongfu ^[1]; Wang, Jingwen ^[1]; Haning, Katie ^[2]; Hu, Yun ^[1]; Wu, Bo ^[3]; He, Mingxiong ^[3]; Zhang, Yaoping ^[4]; Bao, Jie ^[5]; Contreras, Lydia M. ^[2]; Yang, Shihui ^[1]

Hubei Univ., Wuhan (China). College of Life Sciences, Hubei Collaborative Innovation Center for Green Transformation of Bioresources, Environmental Microbial Technology Center and Hubei Key Lab. of Industrial Biotechnology
Univ. of Texas, Austin, TX (United States). Cockrell School of Engineering, Dept. of Chemical Engineering and Inst. for Cellular and Molecular Biology
Biogas Inst. of the Ministry of Agriculture (BIOMA), Chengdu (China). Biomass Energy Technology Research Centre and Key Lab. of Development and Application of Rural Renewable Energy
Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)
East China Univ. of Science and Technology, Shanghai (China). State Key Lab. of Bioreactor Engineering

Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this paper, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries. We also discuss a few metabolic engineering strategies that can be applied in Z. mobilis to further develop it as a robust workhorse for economic lignocellulosic bioproducts. In addition, we briefly review the progress of metabolic engineering in Z. mobilis related to the classical synthetic biology cycle of “Design-Build-Test-Learn”, as well as the progress and potential to develop Z. mobilis as a model chassis for biorefinery practices in the synthetic biology era.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Welch Foundation

Grant/Contract Number:: SC0018409; CBET-1254754; F-1756; DGE-1610403; FC02-07ER64494; BER DE-FC02-07ER64494

OSTI ID:: 1459643

Alternate ID(s):: OSTI ID: 1819008

Journal Information:: Metabolic Engineering, Vol. 50; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 69 works

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Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering Zheng, Yanli; Han, Jiamei; Wang, Baiyang Nucleic Acids Research, Vol. 47, Issue 21 https://doi.org/10.1093/nar/gkz940	journal	October 2019
Applying a New REFINE Approach in Zymomonas mobilis Identifies Novel sRNAs That Confer Improved Stress Tolerance Phenotypes Haning, Katie; Engels, Sean M.; Williams, Paige Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02987	journal	January 2020
Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis Wu, Bo; Qin, Han; Yang, Yiwei Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-018-1348-9	journal	January 2019
Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era Yang, Yongfu; Shen, Wei; Huang, Ju Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1399-6	journal	March 2019
A Markerless Method for Genome Engineering in Zymomonas mobilis ZM4 Lal, Piyush Behari; Wells, Fritz M.; Lyu, Yucai Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02216	journal	October 2019
Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case of Zymomonas mobilis Kalnenieks, Uldis; Balodite, Elina; Rutkis, Reinis Frontiers in Bioengineering and Biotechnology, Vol. 7 https://doi.org/10.3389/fbioe.2019.00327	journal	November 2019
Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions Yang, Shihui; Franden, Mary Ann; Wang, Xia Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00013	journal	January 2020
Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis Shen, Wei; Zhang, Jun; Geng, Binan Microbial Cell Factories, Vol. 18, Issue 1 https://doi.org/10.1186/s12934-019-1219-5	journal	October 2019
Identification and characterization of ethanol‐inducible promoters of Zymomonas mobilis based on omics data and dual reporter–gene system Yang, Yongfu; Rong, Ziyue; Song, Haoyue Biotechnology and Applied Biochemistry https://doi.org/10.1002/bab.1838	journal	December 2019
Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production Qiu, Mengyue; Shen, Wei; Yan, Xiongyin Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-1654-x	journal	January 2020

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