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Title: Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment

Abstract

The application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was thus possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.

Authors:
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Ruhr Univ., Bochum (Germany). Analytical Chemistry-Biointerfaces
  2. Helmholtz-Zentrum Geesthacht (HZG), (Germany). German Research Centre for Materials and Energy
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  4. Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division
  5. St. George's Univ. of London (SGUL) (United Kingdom). Inst. of Infection and Immunity
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Scientific User Facilities Division; Helmholtz Association of German Research Centres, Hamburg (Germany). German Electron Synchrotron (DESY) PETRA III and Helmholtz Virtual Institute (VI); German Research Foundation (DFG); National Science Foundation (NSF)
OSTI Identifier:
1459123
Report Number(s):
LLNL-JRNL-748354
Journal ID: ISSN 1600-5767; JACGAR; 930441
Grant/Contract Number:  
AC52-07NA27344; NSF/CHE-1346572; AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 49; Journal Issue: 6; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY; Escherichia coli ultrastructure; antibiotics; small-angle X-ray scattering; SAXS; ultra-small-angle X-ray scattering; USAXS; transmission electron microscopy; TEM

Citation Formats

von Gundlach, A. R., Garamus, V. M., Willey, T. M., Ilavsky, J., Hilpert, K., and Rosenhahn, A.. Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment. United States: N. p., 2016. Web. doi:10.1107/S1600576716018562.
von Gundlach, A. R., Garamus, V. M., Willey, T. M., Ilavsky, J., Hilpert, K., & Rosenhahn, A.. Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment. United States. doi:10.1107/S1600576716018562.
von Gundlach, A. R., Garamus, V. M., Willey, T. M., Ilavsky, J., Hilpert, K., and Rosenhahn, A.. Thu . "Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment". United States. doi:10.1107/S1600576716018562. https://www.osti.gov/servlets/purl/1459123.
@article{osti_1459123,
title = {Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment},
author = {von Gundlach, A. R. and Garamus, V. M. and Willey, T. M. and Ilavsky, J. and Hilpert, K. and Rosenhahn, A.},
abstractNote = {The application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was thus possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.},
doi = {10.1107/S1600576716018562},
journal = {Journal of Applied Crystallography (Online)},
number = 6,
volume = 49,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 2016},
month = {Thu Dec 01 00:00:00 EST 2016}
}

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Works referenced in this record:

Structural Mechanism for Rifampicin Inhibition of Bacterial RNA Polymerase
journal, March 2001