Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment

von Gundlach, A. R.; Garamus, V. M.; Willey, T. M.; Ilavsky, J.; Hilpert, K.; Rosenhahn, A.

doi:10.1107/S1600576716018562

Title: Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment

Journal Article · Thu Dec 01 00:00:00 EST 2016 · Journal of Applied Crystallography (Online)

DOI:https://doi.org/10.1107/S1600576716018562· OSTI ID:1459123

von Gundlach, A. R. ^[1]; Garamus, V. M. ^[2]; Willey, T. M. ^[3]; Ilavsky, J. ^[4]; Hilpert, K. ^[5]; Rosenhahn, A. ^[1]

Ruhr Univ., Bochum (Germany). Analytical Chemistry-Biointerfaces
Helmholtz-Zentrum Geesthacht (HZG), (Germany). German Research Centre for Materials and Energy
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division
St. George's Univ. of London (SGUL) (United Kingdom). Inst. of Infection and Immunity

The application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was thus possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.

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Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; Helmholtz Association of German Research Centres, Hamburg (Germany). German Electron Synchrotron (DESY) PETRA III and Helmholtz Virtual Institute (VI); German Research Foundation (DFG); National Science Foundation (NSF)

Grant/Contract Number:: AC52-07NA27344; NSF/CHE-1346572; AC02-06CH11357

OSTI ID:: 1459123

Report Number(s):: LLNL-JRNL-748354; JACGAR; 930441; TRN: US1901542

Journal Information:: Journal of Applied Crystallography (Online), Vol. 49, Issue 6; ISSN 1600-5767

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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Mechanisms of Action for Antimicrobial Peptides With Antibacterial and Antibiofilm Functions Raheem, Nigare; Straus, Suzana K. Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02866	journal	December 2019
BioSAXS Measurements Reveal That Two Antimicrobial Peptides Induce Similar Molecular Changes in Gram-Negative and Gram-Positive Bacteria von Gundlach, Andreas; Ashby, Martin P.; Gani, Jurnorain Frontiers in Pharmacology, Vol. 10 https://doi.org/10.3389/fphar.2019.01127	journal	September 2019
BioSAXS Measurements Reveal That Two Antimicrobial Peptides Induce Similar Molecular Changes in Gram-Negative and Gram-Positive Bacteria Von Gundlach, Andreas; Ashby, Martin P.; Gani, Jurnorain Karlsruhe https://doi.org/10.5445/ir/1000098830	text	January 2019

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Table 1(p. 6)

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Related Subjects

60 APPLIED LIFE SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
Escherichia coli ultrastructure
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small-angle X-ray scattering
SAXS
ultra-small-angle X-ray scattering
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transmission electron microscopy
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