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Title: The Next Generation of Split-Fluorescent Proteins and What They Can Do for You

Abstract

A symmetrically split fluorescent protein (split-FP) systems provide unique advantages over conventional fluorophores as well as whole fluorescent proteins for cellular imaging and biochemical assays. In this presentation I will discuss some of these advantages, as well as new optimizations and applications for existing LANL split-FP tools in the emerging field of intrinsically disordered protein (IDP) biology, host-pathogen interactions and materials science. I will also briefly present additions to the LANL split-FP toolbox, such as a new split red fluorescent protein (a vast improvement over the current state of the art) and a new technique to develop multimerization-resistant proteins. Lastly, I will discuss some use-cases for these new materials.

Authors:
 [1];  [1];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1457316
Report Number(s):
LA-UR-18-25520
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
Biological Science

Citation Formats

Banerjee, Shounak, Waldo, Geoffrey S., and Strauss, Charlie E. The Next Generation of Split-Fluorescent Proteins and What They Can Do for You. United States: N. p., 2018. Web. doi:10.2172/1457316.
Banerjee, Shounak, Waldo, Geoffrey S., & Strauss, Charlie E. The Next Generation of Split-Fluorescent Proteins and What They Can Do for You. United States. doi:10.2172/1457316.
Banerjee, Shounak, Waldo, Geoffrey S., and Strauss, Charlie E. Fri . "The Next Generation of Split-Fluorescent Proteins and What They Can Do for You". United States. doi:10.2172/1457316. https://www.osti.gov/servlets/purl/1457316.
@article{osti_1457316,
title = {The Next Generation of Split-Fluorescent Proteins and What They Can Do for You},
author = {Banerjee, Shounak and Waldo, Geoffrey S. and Strauss, Charlie E.},
abstractNote = {A symmetrically split fluorescent protein (split-FP) systems provide unique advantages over conventional fluorophores as well as whole fluorescent proteins for cellular imaging and biochemical assays. In this presentation I will discuss some of these advantages, as well as new optimizations and applications for existing LANL split-FP tools in the emerging field of intrinsically disordered protein (IDP) biology, host-pathogen interactions and materials science. I will also briefly present additions to the LANL split-FP toolbox, such as a new split red fluorescent protein (a vast improvement over the current state of the art) and a new technique to develop multimerization-resistant proteins. Lastly, I will discuss some use-cases for these new materials.},
doi = {10.2172/1457316},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jun 22 00:00:00 EDT 2018},
month = {Fri Jun 22 00:00:00 EDT 2018}
}

Technical Report:

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