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Methods of expanding bacteriophage host-range and bacteriophage produced by the methods

Patent ·
OSTI ID:1456914
;
A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage.
Research Organization:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC04-94AL85000
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
Patent Number(s):
9,988,608
Application Number:
14/883,366
OSTI ID:
1456914
Country of Publication:
United States
Language:
English

References (11)

Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157:H7 journal January 2005
Old dogma, new tricks—21st Century phage therapy journal January 2004
Bacteriophage T4 Host Range is Expanded by Duplications of a Small Domain of the Tail Fiber Adhesin journal May 1996
Numerical Taxonomy of Clostridium botulinum and Clostridium sporogenes Strains, and Their Susceptibilities to Induced Lysins and to Mitomycin C journal May 1973
Genomic Sequence of Bacteriophage ATCC 8074-B1 and Activity of Its Endolysin and Engineered Variants against Clostridium sporogenes journal March 2012
Basic concepts in RNA virus evolution. journal June 1996
Genome plasticity in the distal tail fiber locus of the T-even bacteriophage: recombination between conserved motifs swaps adhesin specificity journal September 1998
DNA sequence of genes 38 encoding a receptor-recognizing protein of bacteriophages T2, K3 and of K3 host range mutants journal March 1987
Effect of mitomycin C on interactions between temperate phages and bacteria journal April 1961
Nucleoside Analogue Mutagenesis of a Single-Stranded DNA Virus: Evolution and Resistance journal June 2012
Arbovirus evolution in vivo is constrained by host alternation journal May 2008

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