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Title: Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut

Abstract

Salmonella entericaelicits intestinal inflammation to gain access to nutrients. One of these nutrients is fructose-asparagine (F-Asn). The availability of F-Asn toSalmonelladuring infection is dependent uponSalmonellapathogenicity islands 1 and 2, which in turn are required to provoke inflammation. Here, we determined that F-Asn is present in mouse chow at approximately 400 pmol/mg (dry weight). F-Asn is also present in the intestinal tract of germfree mice at 2,700 pmol/mg (dry weight) and in the intestinal tract of conventional mice at 9 to 28 pmol/mg. These findings suggest that the mouse intestinal microbiota consumes F-Asn. We utilized heavy-labeled precursors of F-Asn to monitor its formation in the intestine, in the presence or absence of inflammation, and none was observed. Finally, we determined that some members of the classClostridiaencode F-Asn utilization pathways and that they are eliminated from highly inflamedSalmonella-infected mice. Collectively, our studies identify the source of F-Asn as the diet and thatSalmonella-mediated inflammation is required to eliminate competitors and allow the pathogen nearly exclusive access to this nutrient.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1455304
Report Number(s):
PNNL-SA-129012
Journal ID: ISSN 0019-9567
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Infection and Immunity
Additional Journal Information:
Journal Volume: 86; Journal Issue: 5; Journal ID: ISSN 0019-9567
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English

Citation Formats

Wu, Jikang, Sabag-Daigle, Anice, Borton, Mikayla A., Kop, Linnea F. M., Szkoda, Blake E., Deatherage Kaiser, Brooke L., Lindemann, Stephen R., Renslow, Ryan S., Wei, Siwei, Nicora, Carrie D., Weitz, Karl K., Kim, Young-Mo, Adkins, Joshua N., Metz, Thomas O., Boyaka, Prosper, Gopalan, Venkat, Wrighton, Kelly C., Wysocki, Vicki H., Ahmer, Brian M. M., and Young, Vincent B. Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut. United States: N. p., 2018. Web. doi:10.1128/IAI.00945-17.
Wu, Jikang, Sabag-Daigle, Anice, Borton, Mikayla A., Kop, Linnea F. M., Szkoda, Blake E., Deatherage Kaiser, Brooke L., Lindemann, Stephen R., Renslow, Ryan S., Wei, Siwei, Nicora, Carrie D., Weitz, Karl K., Kim, Young-Mo, Adkins, Joshua N., Metz, Thomas O., Boyaka, Prosper, Gopalan, Venkat, Wrighton, Kelly C., Wysocki, Vicki H., Ahmer, Brian M. M., & Young, Vincent B. Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut. United States. doi:10.1128/IAI.00945-17.
Wu, Jikang, Sabag-Daigle, Anice, Borton, Mikayla A., Kop, Linnea F. M., Szkoda, Blake E., Deatherage Kaiser, Brooke L., Lindemann, Stephen R., Renslow, Ryan S., Wei, Siwei, Nicora, Carrie D., Weitz, Karl K., Kim, Young-Mo, Adkins, Joshua N., Metz, Thomas O., Boyaka, Prosper, Gopalan, Venkat, Wrighton, Kelly C., Wysocki, Vicki H., Ahmer, Brian M. M., and Young, Vincent B. Mon . "Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut". United States. doi:10.1128/IAI.00945-17.
@article{osti_1455304,
title = {Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut},
author = {Wu, Jikang and Sabag-Daigle, Anice and Borton, Mikayla A. and Kop, Linnea F. M. and Szkoda, Blake E. and Deatherage Kaiser, Brooke L. and Lindemann, Stephen R. and Renslow, Ryan S. and Wei, Siwei and Nicora, Carrie D. and Weitz, Karl K. and Kim, Young-Mo and Adkins, Joshua N. and Metz, Thomas O. and Boyaka, Prosper and Gopalan, Venkat and Wrighton, Kelly C. and Wysocki, Vicki H. and Ahmer, Brian M. M. and Young, Vincent B.},
abstractNote = {Salmonella entericaelicits intestinal inflammation to gain access to nutrients. One of these nutrients is fructose-asparagine (F-Asn). The availability of F-Asn toSalmonelladuring infection is dependent uponSalmonellapathogenicity islands 1 and 2, which in turn are required to provoke inflammation. Here, we determined that F-Asn is present in mouse chow at approximately 400 pmol/mg (dry weight). F-Asn is also present in the intestinal tract of germfree mice at 2,700 pmol/mg (dry weight) and in the intestinal tract of conventional mice at 9 to 28 pmol/mg. These findings suggest that the mouse intestinal microbiota consumes F-Asn. We utilized heavy-labeled precursors of F-Asn to monitor its formation in the intestine, in the presence or absence of inflammation, and none was observed. Finally, we determined that some members of the classClostridiaencode F-Asn utilization pathways and that they are eliminated from highly inflamedSalmonella-infected mice. Collectively, our studies identify the source of F-Asn as the diet and thatSalmonella-mediated inflammation is required to eliminate competitors and allow the pathogen nearly exclusive access to this nutrient.},
doi = {10.1128/IAI.00945-17},
journal = {Infection and Immunity},
issn = {0019-9567},
number = 5,
volume = 86,
place = {United States},
year = {2018},
month = {2}
}