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Title: Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry

Abstract

Lipid mediators (LMs) are broadly defined as a class of bioactive lipophilic molecules that regulate cell-to-cell communication events with many having a strong correlation with various human diseases and conditions. LMs are usually analyzed with liquid chromatography and mass spectrometry (LC-MS), but their numerous isomers greatly complicate the measurements with essentially identical fragmentation spectra and LC separations not always sufficient for distinguishing the features. In this work, we characterized LMs having specific categories using ion mobility spectrometry coupled with mass spectrometry (IMS-MS). The IMS collision cross sections and MS m/z values displayed distinct trends for each LM category studied. LC-IMS-MS analyses on flu infected mouse tissue samples also illustrated the presence of additional LM species not in our databases.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1455278
Report Number(s):
PNNL-SA-130733
Journal ID: ISSN 1757-6180; 49531; 400412000
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Bioanalysis
Additional Journal Information:
Journal Volume: 10; Journal Issue: 5; Journal ID: ISSN 1757-6180
Publisher:
Future Science Group
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Kyle, Jennifer E., Aly, Noor, Zheng, Xueyun, Burnum-Johnson, Kristin E., Smith, Richard D., and Baker, Erin S. Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry. United States: N. p., 2018. Web. doi:10.4155/bio-2017-0245.
Kyle, Jennifer E., Aly, Noor, Zheng, Xueyun, Burnum-Johnson, Kristin E., Smith, Richard D., & Baker, Erin S. Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry. United States. doi:10.4155/bio-2017-0245.
Kyle, Jennifer E., Aly, Noor, Zheng, Xueyun, Burnum-Johnson, Kristin E., Smith, Richard D., and Baker, Erin S. Thu . "Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry". United States. doi:10.4155/bio-2017-0245.
@article{osti_1455278,
title = {Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry},
author = {Kyle, Jennifer E. and Aly, Noor and Zheng, Xueyun and Burnum-Johnson, Kristin E. and Smith, Richard D. and Baker, Erin S.},
abstractNote = {Lipid mediators (LMs) are broadly defined as a class of bioactive lipophilic molecules that regulate cell-to-cell communication events with many having a strong correlation with various human diseases and conditions. LMs are usually analyzed with liquid chromatography and mass spectrometry (LC-MS), but their numerous isomers greatly complicate the measurements with essentially identical fragmentation spectra and LC separations not always sufficient for distinguishing the features. In this work, we characterized LMs having specific categories using ion mobility spectrometry coupled with mass spectrometry (IMS-MS). The IMS collision cross sections and MS m/z values displayed distinct trends for each LM category studied. LC-IMS-MS analyses on flu infected mouse tissue samples also illustrated the presence of additional LM species not in our databases.},
doi = {10.4155/bio-2017-0245},
journal = {Bioanalysis},
issn = {1757-6180},
number = 5,
volume = 10,
place = {United States},
year = {2018},
month = {3}
}