Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry

Kyle, Jennifer E.; Aly, Noor; Zheng, Xueyun; Burnum-Johnson, Kristin E.; Smith, Richard D.; Baker, Erin S.

doi:10.4155/bio-2017-0245

Title: Evaluating lipid mediator structural complexity using ion mobility spectrometry combined with mass spectrometry

Journal Article · Thu Mar 01 00:00:00 EST 2018 · Bioanalysis

DOI:https://doi.org/10.4155/bio-2017-0245· OSTI ID:1455278

Kyle, Jennifer E. ^[1]; Aly, Noor ^[1]; Zheng, Xueyun ^[1]; Burnum-Johnson, Kristin E. ^[1]; Smith, Richard D. ^[1]; Baker, Erin S. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Lipid mediators (LMs) are broadly defined as a class of bioactive lipophilic molecules that regulate cell-to-cell communication events with many having a strong correlation with various human diseases and conditions. LMs are usually analyzed with liquid chromatography and mass spectrometry (LC-MS), but their numerous isomers greatly complicate the measurements with essentially identical fragmentation spectra and LC separations not always sufficient for distinguishing the features. Herein, we characterized LMs having specific categories using ion mobility spectrometry coupled with mass spectrometry (IMS-MS). The collision cross-sections and m/z values from the IMS and MS analyses displayed distinct trend lines. Specifically, the structural trend lines for sodiated LMs originating from docosahexaenoic acid had the smallest collision cross-section values in relation to m/z, while those from linoleic acid had the largest. LC–IMS–MS analyses were also performed on LMs in flu infected mouse tissue samples. These multidimensional studies were able to assess known LMs while also detecting new species. Adding IMS separations to conventional LC–MS analyses show great utility for enabling better identification and characterization of LMs in complex biological samples.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: National Institute of Environmental Health Sciences (NIEHS); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); National Institute of Allergy and Infectious Diseases (NIAID); USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; R01 ES022190; P41 GM103493; U19AI106772

OSTI ID:: 1455278

Report Number(s):: PNNL-SA-130733; 49531; 400412000

Journal Information:: Bioanalysis, Vol. 10, Issue 5; ISSN 1757-6180

Publisher:: Future Science GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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References (40)

Lipid Mediators in Health and Disease: Enzymes and Receptors as Therapeutic Targets for the Regulation of Immunity and Inflammation Shimizu, Takao Annual Review of Pharmacology and Toxicology, Vol. 49, Issue 1 https://doi.org/10.1146/annurev.pharmtox.011008.145616	journal	February 2009
Coupling Front-End Separations, Ion Mobility Spectrometry, and Mass Spectrometry For Enhanced Multidimensional Biological and Environmental Analyses Zheng, Xueyun; Wojcik, Roza; Zhang, Xing Annual Review of Analytical Chemistry, Vol. 10, Issue 1 https://doi.org/10.1146/annurev-anchem-061516-045212	journal	June 2017
The Lipid Mediator Protectin D1 Inhibits Influenza Virus Replication and Improves Severe Influenza Morita, Masayuki; Kuba, Keiji; Ichikawa, Akihiko Cell, Vol. 153, Issue 1 https://doi.org/10.1016/j.cell.2013.02.027	journal	March 2013
LC-IMS-MS Feature Finder: detecting multidimensional liquid chromatography, ion mobility and mass spectrometry features in complex datasets Crowell, Kevin L.; Slysz, Gordon W.; Baker, Erin S. Bioinformatics, Vol. 29, Issue 21 https://doi.org/10.1093/bioinformatics/btt465	journal	September 2013
Three-dimensional enhanced lipidomics analysis combining UPLC, differential ion mobility spectrometry, and mass spectrometric separation strategies Baker, Paul R. S.; Armando, Aaron M.; Campbell, J. Larry Journal of Lipid Research, Vol. 55, Issue 11 https://doi.org/10.1194/jlr.D051581	journal	September 2014
Ion Mobility Collision Cross Section Compendium May, Jody C.; Morris, Caleb B.; McLean, John A. Analytical Chemistry, Vol. 89, Issue 2 https://doi.org/10.1021/acs.analchem.6b04905	journal	December 2016
Comparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies: Comparing lipids and metabolites in serum and DBS samples Kyle, Jennifer E.; Casey, Cameron P.; Stratton, Kelly G. Rapid Communications in Mass Spectrometry, Vol. 31, Issue 5 https://doi.org/10.1002/rcm.7808	journal	February 2017
Identification and signature profiles for pro-resolving and inflammatory lipid mediators in human tissue Colas, Romain A.; Shinohara, Masakazu; Dalli, Jesmond American Journal of Physiology-Cell Physiology, Vol. 307, Issue 1 https://doi.org/10.1152/ajpcell.00024.2014	journal	July 2014
Applications of ion-mobility mass spectrometry for lipid analysis Paglia, Giuseppe; Kliman, Michal; Claude, Emmanuelle Analytical and Bioanalytical Chemistry, Vol. 407, Issue 17 https://doi.org/10.1007/s00216-015-8664-8	journal	April 2015
A structural examination and collision cross section database for over 500 metabolites and xenobiotics using drift tube ion mobility spectrometry Zheng, Xueyun; Aly, Noor A.; Zhou, Yuxuan Chem. Sci., Vol. 8, Issue 11 https://doi.org/10.1039/C7SC03464D	journal	January 2017
A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements Orton, Daniel J.; Tfaily, Malak M.; Moore, Ronald J. Analytical Chemistry, Vol. 90, Issue 1 https://doi.org/10.1021/acs.analchem.7b02986	journal	December 2017
Enhancing bottom-up and top-down proteomic measurements with ion mobility separations Baker, Erin Shammel; Burnum-Johnson, Kristin E.; Ibrahim, Yehia M. PROTEOMICS, Vol. 15, Issue 16 https://doi.org/10.1002/pmic.201500048	journal	July 2015
Lipid and Glycolipid Isomer Analyses Using Ultra-High Resolution Ion Mobility Spectrometry Separations Wojcik, Roza; Webb, Ian; Deng, Liulin International Journal of Molecular Sciences, Vol. 18, Issue 1 https://doi.org/10.3390/ijms18010183	journal	January 2017
A cluster of immunoresolvents links coagulation to innate host defense in human blood Norris, Paul C.; Libreros, Stephania; Chiang, Nan Science Signaling, Vol. 10, Issue 490 https://doi.org/10.1126/scisignal.aan1471	journal	August 2017
MPLEx: a Robust and Universal Protocol for Single-Sample Integrative Proteomic, Metabolomic, and Lipidomic Analyses Nakayasu, Ernesto S.; Nicora, Carrie D.; Sims, Amy C. mSystems, Vol. 1, Issue 3 https://doi.org/10.1128/mSystems.00043-16	journal	May 2016
Conformational Ordering of Biomolecules in the Gas Phase: Nitrogen Collision Cross Sections Measured on a Prototype High Resolution Drift Tube Ion Mobility-Mass Spectrometer May, Jody C.; Goodwin, Cody R.; Lareau, Nichole M. Analytical Chemistry, Vol. 86, Issue 4, p. 2107-2116 https://doi.org/10.1021/ac4038448	journal	February 2014
Three-Dimensional Ion Mobility/TOFMS Analysis of Electrosprayed Biomolecules Hoaglund, Cherokee S.; Valentine, Stephen J.; Sporleder, C. Ray Analytical Chemistry, Vol. 70, Issue 11 https://doi.org/10.1021/ac980059c	journal	June 1998
Advancing the High Throughput Identification of Liver Fibrosis Protein Signatures Using Multiplexed Ion Mobility Spectrometry Baker, Erin Shammel; Burnum-Johnson, Kristin E.; Jacobs, Jon M. Molecular & Cellular Proteomics, Vol. 13, Issue 4, p. 1119-1127 https://doi.org/10.1074/mcp.M113.034595	journal	January 2014
Structural Elucidation of cis / trans Dicaffeoylquinic Acid Photoisomerization Using Ion Mobility Spectrometry-Mass Spectrometry Zheng, Xueyun; Renslow, Ryan S.; Makola, Mpho M. The Journal of Physical Chemistry Letters, Vol. 8, Issue 7 https://doi.org/10.1021/acs.jpclett.6b03015	journal	March 2017
Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry Kyle, Jennifer E.; Zhang, Xing; Weitz, Karl K. The Analyst, Vol. 141, Issue 5 https://doi.org/10.1039/C5AN02062J	journal	January 2016
Lipid Mediators in Life Science Murakami, Makoto Experimental Animals, Vol. 60, Issue 1 https://doi.org/10.1538/expanim.60.7	journal	January 2011
High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids Groessl, M.; Graf, S.; Knochenmuss, R. The Analyst, Vol. 140, Issue 20 https://doi.org/10.1039/C5AN00838G	journal	January 2015
Distinguishing d - and l -aspartic and isoaspartic acids in amyloid β peptides with ultrahigh resolution ion mobility spectrometry Zheng, Xueyun; Deng, Liulin; Baker, Erin S. Chemical Communications, Vol. 53, Issue 56 https://doi.org/10.1039/C7CC03321D	journal	January 2017
Broadscale resolving power performance of a high precision uniform field ion mobility-mass spectrometer May, Jody C.; Dodds, James N.; Kurulugama, Ruwan T. The Analyst, Vol. 140, Issue 20 https://doi.org/10.1039/C5AN00923E	journal	January 2015
Resolvins, Specialized Proresolving Lipid Mediators, and Their Potential Roles in Metabolic Diseases Spite, Matthew; Clària, Joan; Serhan, Charles N. Cell Metabolism, Vol. 19, Issue 1 https://doi.org/10.1016/j.cmet.2013.10.006	journal	January 2014
Leukotriene-E4 in human urine: Comparison of on-line purification and liquid chromatography–tandem mass spectrometry to affinity purification followed by enzyme immunoassay Armstrong, Michael; Liu, Andrew H.; Harbeck, Ronald Journal of Chromatography B, Vol. 877, Issue 27 https://doi.org/10.1016/j.jchromb.2009.08.011	journal	October 2009
Development of a new ion mobility time-of-flight mass spectrometer Ibrahim, Yehia M.; Baker, Erin S.; Danielson, William F. International Journal of Mass Spectrometry, Vol. 377 https://doi.org/10.1016/j.ijms.2014.07.034	journal	February 2015
An LC-IMS-MS Platform Providing Increased Dynamic Range for High-Throughput Proteomic Studies Baker, Erin Shammel; Livesay, Eric A.; Orton, Daniel J. Journal of Proteome Research, Vol. 9, Issue 2 https://doi.org/10.1021/pr900888b	journal	February 2010
Lipidomic Profiling of Influenza Infection Identifies Mediators that Induce and Resolve Inflammation Tam, Vincent C.; Quehenberger, Oswald; Oshansky, Christine M. Cell, Vol. 154, Issue 1 https://doi.org/10.1016/j.cell.2013.05.052	journal	July 2013
Enhanced lipid isomer separation in human plasma using reversed-phase UPLC with ion-mobility/high-resolution MS detection Damen, Carola W. N.; Isaac, Giorgis; Langridge, James Journal of Lipid Research, Vol. 55, Issue 8 https://doi.org/10.1194/jlr.D047795	journal	June 2014
Anti-Inflammatory Actions of Neuroprotectin D1/Protectin D1 and Its Natural Stereoisomers: Assignments of Dihydroxy-Containing Docosatrienes Serhan, Charles N.; Gotlinger, Katherine; Hong, Song The Journal of Immunology, Vol. 176, Issue 3 https://doi.org/10.4049/jimmunol.176.3.1848	journal	January 2006
Comprehensive ultra-performance liquid chromatographic separation and mass spectrometric analysis of eicosanoid metabolites in human samples Wang, Yan; Armando, Aaron M.; Quehenberger, Oswald Journal of Chromatography A, Vol. 1359 https://doi.org/10.1016/j.chroma.2014.07.006	journal	September 2014
Separation and Classification of Lipids Using Differential Ion Mobility Spectrometry Shvartsburg, Alexandre A.; Isaac, Giorgis; Leveque, Nathalie Journal of The American Society for Mass Spectrometry, Vol. 22, Issue 7 https://doi.org/10.1007/s13361-011-0114-z	journal	April 2011
Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung Dautel, Sydney E.; Kyle, Jennifer E.; Clair, Geremy Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/srep40555	journal	February 2017
Biomolecular structural separations by ion mobility–mass spectrometry Fenn, Larissa S.; McLean, John A. Analytical and Bioanalytical Chemistry, Vol. 391, Issue 3 https://doi.org/10.1007/s00216-008-1951-x	journal	March 2008
Lipid analysis and lipidomics by structurally selective ion mobility-mass spectrometry Kliman, Michal; May, Jody C.; McLean, John A. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1811, Issue 11 https://doi.org/10.1016/j.bbalip.2011.05.016	journal	November 2011
Lipidomics reveals a remarkable diversity of lipids in human plasma, Quehenberger, Oswald; Armando, Aaron M.; Brown, Alex H. Journal of Lipid Research, Vol. 51, Issue 11 https://doi.org/10.1194/jlr.M009449	journal	July 2010
Beyond Prostaglandins-Chemistry and Biology of Cyclic Oxygenated Metabolites Formed by Free-Radical Pathways from Polyunsaturated Fatty Acids Jahn, Ullrich; Galano, Jean-Marie; Durand, Thierry Angewandte Chemie International Edition, Vol. 47, Issue 32 https://doi.org/10.1002/anie.200705122	journal	July 2008
Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry Maccarone, Alan T.; Duldig, Jackson; Mitchell, Todd W. Journal of Lipid Research, Vol. 55, Issue 8 https://doi.org/10.1194/jlr.M046995	journal	June 2014
An Interlaboratory Evaluation of Drift Tube Ion Mobility–Mass Spectrometry Collision Cross Section Measurements Stow, Sarah M.; Causon, Tim J.; Zheng, Xueyun Analytical Chemistry, Vol. 89, Issue 17 https://doi.org/10.1021/acs.analchem.7b01729	journal	August 2017

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