Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis
Abstract
Diatoms are unicellular photosynthetic algae with promise for green production of fuels and other chemicals. Recent genome-editing techniques have greatly improved the potential of many eukaryotic genetic systems, including diatoms, to enable knowledge-based studies and bioengineering. Using a new technique, transcription activator-like effector nucleases (TALENs), the gene encoding the urease enzyme in the model diatom, Phaeodactylum tricornutum, was targeted for interruption. The knockout cassette was identified within the urease gene by PCR and Southern blot analyses of genomic DNA. The lack of urease protein was confirmed by Western blot analyses in mutant cell lines that were unable to grow on urea as the sole nitrogen source. Untargeted metabolomic analysis revealed a build-up of urea, arginine and ornithine in the urease knockout lines. All three intermediate metabolites are upstream of the urease reaction within the urea cycle, suggesting a disruption of the cycle despite urea production. Numerous high carbon metabolites were enriched in the mutant, implying a breakdown of cellular C and N repartitioning. The presented method improves the molecular toolkit for diatoms and clarifies the role of urease in the urea cycle.
- Authors:
-
- Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla CA USA
- Department of Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla CA USA
- Proteomics and Metabolomics Facility, Colorado State University, Fort Collins CO USA
- Department of Biology, Colorado State University, Fort Collins CO USA
- Department of Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla CA USA, Scripps Institution of Oceanography, Integrative Oceanography Division, La Jolla CA USA
- Publication Date:
- Research Org.:
- J. Craig Venter Inst., La Jolla, CA (United States); Colorado State Univ., Fort Collins, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Gordon and Betty Moore Foundation (United States)
- OSTI Identifier:
- 1237106
- Alternate Identifier(s):
- OSTI ID: 1237107; OSTI ID: 1455144
- Grant/Contract Number:
- SC0008593; SC0006719; SC0008595; MCB-1024913; MCB-1129303; GBMF3828
- Resource Type:
- Journal Article: Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 13 Journal Issue: 4; Journal ID: ISSN 1467-7644
- Publisher:
- Society for Experimental Biology; Association of Applied Biology
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; diatoms; genome editing; urease; metabolomics; Phaeodactylum tricornutum; transcription activator-like effector nucleases
Citation Formats
Weyman, Philip D., Beeri, Karen, Lefebvre, Stephane C., Rivera, Josefa, McCarthy, James K., Heuberger, Adam L., Peers, Graham, Allen, Andrew E., and Dupont, Christopher L. Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis. United Kingdom: N. p., 2014.
Web. doi:10.1111/pbi.12254.
Weyman, Philip D., Beeri, Karen, Lefebvre, Stephane C., Rivera, Josefa, McCarthy, James K., Heuberger, Adam L., Peers, Graham, Allen, Andrew E., & Dupont, Christopher L. Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis. United Kingdom. https://doi.org/10.1111/pbi.12254
Weyman, Philip D., Beeri, Karen, Lefebvre, Stephane C., Rivera, Josefa, McCarthy, James K., Heuberger, Adam L., Peers, Graham, Allen, Andrew E., and Dupont, Christopher L. 2014.
"Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis". United Kingdom. https://doi.org/10.1111/pbi.12254.
@article{osti_1237106,
title = {Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis},
author = {Weyman, Philip D. and Beeri, Karen and Lefebvre, Stephane C. and Rivera, Josefa and McCarthy, James K. and Heuberger, Adam L. and Peers, Graham and Allen, Andrew E. and Dupont, Christopher L.},
abstractNote = {Diatoms are unicellular photosynthetic algae with promise for green production of fuels and other chemicals. Recent genome-editing techniques have greatly improved the potential of many eukaryotic genetic systems, including diatoms, to enable knowledge-based studies and bioengineering. Using a new technique, transcription activator-like effector nucleases (TALENs), the gene encoding the urease enzyme in the model diatom, Phaeodactylum tricornutum, was targeted for interruption. The knockout cassette was identified within the urease gene by PCR and Southern blot analyses of genomic DNA. The lack of urease protein was confirmed by Western blot analyses in mutant cell lines that were unable to grow on urea as the sole nitrogen source. Untargeted metabolomic analysis revealed a build-up of urea, arginine and ornithine in the urease knockout lines. All three intermediate metabolites are upstream of the urease reaction within the urea cycle, suggesting a disruption of the cycle despite urea production. Numerous high carbon metabolites were enriched in the mutant, implying a breakdown of cellular C and N repartitioning. The presented method improves the molecular toolkit for diatoms and clarifies the role of urease in the urea cycle.},
doi = {10.1111/pbi.12254},
url = {https://www.osti.gov/biblio/1237106},
journal = {Plant Biotechnology Journal},
issn = {1467-7644},
number = 4,
volume = 13,
place = {United Kingdom},
year = {Fri Oct 10 00:00:00 EDT 2014},
month = {Fri Oct 10 00:00:00 EDT 2014}
}
Web of Science
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