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Title: Transformable Rhodobacter strains, method for producing transformable Rhodobacter strains

Patent ·
OSTI ID:1440541

The invention provides an organism for expressing foreign DNA, the organism engineered to accept standard DNA carriers. The genome of the organism codes for intracytoplasmic membranes and features an interruption in at least one of the genes coding for restriction enzymes. Further provided is a system for producing biological materials comprising: selecting a vehicle to carry DNA which codes for the biological materials; determining sites on the vehicle's DNA sequence susceptible to restriction enzyme cleavage; choosing an organism to accept the vehicle based on that organism not acting upon at least one of said vehicle's sites; engineering said vehicle to contain said DNA; thereby creating a synthetic vector; and causing the synthetic vector to enter the organism so as cause expression of said DNA.

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-06CH11357
Assignee:
UChicago Argonne, LLC (Chicago, IL)
Patent Number(s):
9,963,709
Application Number:
13/619,406
OSTI ID:
1440541
Resource Relation:
Patent File Date: 2012 Sep 14
Country of Publication:
United States
Language:
English

References (10)

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Viable Gram Negative Bacteria with Reduced Proteolytic Activity Lacking Outer Membrane Agonists of TLR4/MD-2 patent-application August 2014
[22] Genetic techniques in rhodospirillaceae book January 1991
Postgenomic Adventures with Rhodobacter sphaeroides journal October 2007
Genome Analyses of Three Strains of Rhodobacter sphaeroides: Evidence of Rapid Evolution of Chromosome II journal December 2006