skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Abstract

A method is disclosed for analyzing a thin tissue sample and adapted to be supported on a slide. The tissue sample may be placed on a slide and exposed to one or more different exogenous fluorophores excitable in a range of about 300 nm-200 nm, and having a useful emission band from about 350 nm-900 nm, and including one or more fluorescent dyes or fluorescently labeled molecular probes that accumulate in tissue or cellular components. The fluorophores may be excited with a first wavelength of UV light between about 200 nm-290 nm. An optical system collects emissions from the fluorophores at a second wavelength, different from the first wavelength, which are generated in response to the first wavelength of UV light, to produce an image for analysis.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1440530
Patent Number(s):
9,964,489
Application Number:
15/466,195
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 Mar 22
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Levenson, Richard, and Demos, Stavros. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents. United States: N. p., 2018. Web.
Levenson, Richard, & Demos, Stavros. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents. United States.
Levenson, Richard, and Demos, Stavros. Tue . "System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents". United States. https://www.osti.gov/servlets/purl/1440530.
@article{osti_1440530,
title = {System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents},
author = {Levenson, Richard and Demos, Stavros},
abstractNote = {A method is disclosed for analyzing a thin tissue sample and adapted to be supported on a slide. The tissue sample may be placed on a slide and exposed to one or more different exogenous fluorophores excitable in a range of about 300 nm-200 nm, and having a useful emission band from about 350 nm-900 nm, and including one or more fluorescent dyes or fluorescently labeled molecular probes that accumulate in tissue or cellular components. The fluorophores may be excited with a first wavelength of UV light between about 200 nm-290 nm. An optical system collects emissions from the fluorophores at a second wavelength, different from the first wavelength, which are generated in response to the first wavelength of UV light, to produce an image for analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
journal, January 2009

  • Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M. G.
  • Optics Express, Vol. 17, Issue 15, p. 12502-12509
  • DOI: 10.1364/OE.17.012502

Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence
journal, September 2010

  • Bhartia, R.; Salas, E. C.; Hug, W. F.
  • Applied and Environmental Microbiology, Vol. 76, Issue 21, p. 7231-7237
  • DOI: 10.1128/AEM.00943-10

Deep UV autofluorescence microscopy for cell biology and tissue histology Deep UV autofluorescence microscopy
journal, April 2013

  • Jamme, Fr�d�ric; Kascakova, Slavka; Villette, Sandrine
  • Biology of the Cell, Vol. 105, Issue 7, p. 277-288
  • DOI: 10.1111/boc.201200075

Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging
conference, March 2015


Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device
journal, July 2003


Characterizing the origin of autofluorescence in human esophageal epithelium under ultraviolet excitation
journal, January 2010

  • Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M. G.
  • Optics Express, Vol. 18, Issue 20, Article No. 21074
  • DOI: 10.1364/OE.18.021074