Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels
Abstract
Achieving low cost and high efficiency lignocellulose deconstruction is a critical step towards widespread adoption of lignocellulosic biofuels. Certain ionic liquid (IL)-based pretreatment processes effectively reduce recalcitrance of lignocellulose to enzymatic degradation but require either costly separations following pretreatment or novel IL compatible processes to mitigate downstream toxicity. Here we demonstrate at benchtop and pilot bioreactor scales a separation-free, intensified process for IL pretreatment, saccharification, and fermentation of sorghum biomass to produce the sesquiterpene bisabolene, a precursor to the renewable diesel and jet fuel bisabolane. The deconstruction process employs the IL cholinium lysinate ([Ch][Lys]), followed by enzymatic saccharification with the commercial enzyme cocktails Cellic CTec2 and HTec2. Glucose yields above 80% and xylose yields above 60% are observed at all scales tested. Unfiltered hydrolysate is fermented directly by Rhodosporidium toruloides-with glucose, xylose, acetate and lactate fully consumed during fermentation at all scales tested. Bisabolene titers improved with scale from 1.3 g L-1 in 30 mL shake flasks to 2.2 g L-1 in 20 L fermentation. The combined process enables conversion of saccharified IL-pretreated biomass directly to advanced biofuels with no separations or washing, minimal additions to facilitate fermentation, no loss of performance due to IL toxicity, and simplified fuel recoverymore »
- Authors:
-
- Lawrence Berkeley National Laboratory, Berkeley, USA
- Pacific Northwest National Laboratory, Richland, USA, Joint BioEnergy Institute, Emeryville
- Lawrence Berkeley National Laboratory, Berkeley, USA, Joint BioEnergy Institute, Emeryville
- Joint BioEnergy Institute, Emeryville, USA, Sandia National Laboratory, Livermore
- Pacific Northwest National Laboratory, Richland, USA
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1439778
- Alternate Identifier(s):
- OSTI ID: 1458771; OSTI ID: 1506283
- Grant/Contract Number:
- AC02-05CH11231; AC05-76RL01830
- Resource Type:
- Journal Article: Published Article
- Journal Name:
- Green Chemistry
- Additional Journal Information:
- Journal Name: Green Chemistry Journal Volume: 20 Journal Issue: 12; Journal ID: ISSN 1463-9262
- Publisher:
- Royal Society of Chemistry (RSC)
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, and Gladden, John. Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels. United Kingdom: N. p., 2018.
Web. doi:10.1039/C8GC00518D.
Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, & Gladden, John. Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels. United Kingdom. https://doi.org/10.1039/C8GC00518D
Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, and Gladden, John. 2018.
"Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels". United Kingdom. https://doi.org/10.1039/C8GC00518D.
@article{osti_1439778,
title = {Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels},
author = {Sundstrom, Eric and Yaegashi, Junko and Yan, Jipeng and Masson, Fabrice and Papa, Gabriella and Rodriguez, Alberto and Mirsiaghi, Mona and Liang, Ling and He, Qian and Tanjore, Deepti and Pray, Todd R. and Singh, Seema and Simmons, Blake and Sun, Ning and Magnuson, Jon and Gladden, John},
abstractNote = {Achieving low cost and high efficiency lignocellulose deconstruction is a critical step towards widespread adoption of lignocellulosic biofuels. Certain ionic liquid (IL)-based pretreatment processes effectively reduce recalcitrance of lignocellulose to enzymatic degradation but require either costly separations following pretreatment or novel IL compatible processes to mitigate downstream toxicity. Here we demonstrate at benchtop and pilot bioreactor scales a separation-free, intensified process for IL pretreatment, saccharification, and fermentation of sorghum biomass to produce the sesquiterpene bisabolene, a precursor to the renewable diesel and jet fuel bisabolane. The deconstruction process employs the IL cholinium lysinate ([Ch][Lys]), followed by enzymatic saccharification with the commercial enzyme cocktails Cellic CTec2 and HTec2. Glucose yields above 80% and xylose yields above 60% are observed at all scales tested. Unfiltered hydrolysate is fermented directly by Rhodosporidium toruloides-with glucose, xylose, acetate and lactate fully consumed during fermentation at all scales tested. Bisabolene titers improved with scale from 1.3 g L-1 in 30 mL shake flasks to 2.2 g L-1 in 20 L fermentation. The combined process enables conversion of saccharified IL-pretreated biomass directly to advanced biofuels with no separations or washing, minimal additions to facilitate fermentation, no loss of performance due to IL toxicity, and simplified fuel recovery via phase separation. This study is the first to demonstrate a separation-free IL based process for conversion of biomass to an advanced biofuel and is the first to demonstrate full consumption of glucose, xylose, acetate, and lactic acid in the presence of [Ch][Lys].},
doi = {10.1039/C8GC00518D},
url = {https://www.osti.gov/biblio/1439778},
journal = {Green Chemistry},
issn = {1463-9262},
number = 12,
volume = 20,
place = {United Kingdom},
year = {Mon Jan 01 00:00:00 EST 2018},
month = {Mon Jan 01 00:00:00 EST 2018}
}
Web of Science
Figures / Tables:
Works referenced in this record:
The generation of fermentation inhibitors during dilute acid hydrolysis of softwood
journal, February 1999
- Larsson, Simona; Palmqvist, Eva; Hahn-Hägerdal, Bärbel
- Enzyme and Microbial Technology, Vol. 24, Issue 3-4
Biodiesel production from oleaginous microorganisms
journal, January 2009
- Meng, Xin; Yang, Jianming; Xu, Xin
- Renewable Energy, Vol. 34, Issue 1
Ionic liquids from renewable biomaterials: synthesis, characterization and application in the pretreatment of biomass
journal, January 2012
- Liu, Qiu-Ping; Hou, Xue-Dan; Li, Ning
- Green Chem., Vol. 14, Issue 2
A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels
journal, May 2012
- Park, Joshua I.; Steen, Eric J.; Burd, Helcio
- PLoS ONE, Vol. 7, Issue 5, Article No. e37010
Transforming biomass conversion with ionic liquids: process intensification and the development of a high-gravity, one-pot process for the production of cellulosic ethanol
journal, January 2016
- Xu, Feng; Sun, Jian; Konda, N. V. S. N. Murthy
- Energy & Environmental Science, Vol. 9, Issue 3
Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts
journal, October 2017
- Yaegashi, Junko; Kirby, James; Ito, Masakazu
- Biotechnology for Biofuels, Vol. 10, Issue 1
Techno-economic analysis of a lignocellulosic ethanol biorefinery with ionic liquid pre-treatment
journal, June 2011
- Klein-Marcuschamer, Daniel; Simmons, Blake A.; Blanch, Harvey W.
- Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 5
Lipid production in batch and fed-batch cultures of Rhodosporidium toruloidesfrom 5 and 6 carbon carbohydrates
journal, May 2012
- Wiebe, Marilyn G.; Koivuranta, Kari; Penttilä, Merja
- BMC Biotechnology, Vol. 12, Issue 1
Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
journal, January 2013
- Özaydın, Bilge; Burd, Helcio; Lee, Taek Soon
- Metabolic Engineering, Vol. 15
l(+)-Lactic acid production from non-food carbohydrates by thermotolerant Bacillus coagulans
journal, August 2010
- Ou, Mark S.; Ingram, Lonnie O.; Shanmugam, K. T.
- Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 5
Effect of organic acids on the growth and fermentation of ethanologenicEscherichia coli LY01
journal, January 1999
- Zaldivar, Jesus; Ingram, Lonnie O.
- Biotechnology and Bioengineering, Vol. 66, Issue 4
Microbial lipid production from SPORL-pretreated Douglas fir by Mortierella isabellina
journal, July 2016
- Harde, S. M.; Wang, Z.; Horne, M.
- Fuel, Vol. 175
Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides
journal, February 2014
- Lin, Xinping; Wang, Yanan; Zhang, Sufang
- FEMS Yeast Research, Vol. 14, Issue 4
Development of an E. coli strain for one-pot biofuel production from ionic liquid pretreated cellulose and switchgrass
journal, January 2016
- Frederix, Marijke; Mingardon, Florence; Hu, Matthew
- Green Chemistry, Vol. 18, Issue 15
Identification and microbial production of a terpene-based advanced biofuel
journal, September 2011
- Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana
- Nature Communications, Vol. 2, Issue 1
Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification
journal, July 2010
- Li, Chenlin; Knierim, Bernhard; Manisseri, Chithra
- Bioresource Technology, Vol. 101, Issue 13
Dilute acid pretreatment of lignocellulose for whole slurry ethanol fermentation
journal, March 2013
- Jung, Young Hoon; Kim, In Jung; Kim, Hyun Kyung
- Bioresource Technology, Vol. 132
Engineering Rhodosporidium toruloides for increased lipid production : Engineering
journal, November 2015
- Zhang, Shuyan; Skerker, Jeffrey M.; Rutter, Charles D.
- Biotechnology and Bioengineering, Vol. 113, Issue 5
Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step
journal, January 2006
- Dadi, Anantharam P.; Varanasi, Sasidhar; Schall, Constance A.
- Biotechnology and Bioengineering, Vol. 95, Issue 5, p. 904-910
Understanding cost drivers and economic potential of two variants of ionic liquid pretreatment for cellulosic biofuel production
journal, January 2014
- Konda, Nvsn; Shi, Jian; Singh, Seema
- Biotechnology for Biofuels, Vol. 7, Issue 1
Understanding pretreatment efficacy of four cholinium and imidazolium ionic liquids by chemistry and computation
journal, January 2014
- Sun, Ning; Parthasarathi, Ramakrishnan; Socha, Aaron M.
- Green Chem., Vol. 16, Issue 5
Fatty alcohols production by oleaginous yeast
journal, August 2015
- Fillet, Sandy; Gibert, Jordi; Suárez, Beatriz
- Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 11
Engineering Terpene Biosynthesis in Streptomyces for Production of the Advanced Biofuel Precursor Bisabolene
journal, July 2014
- Phelan, Ryan M.; Sekurova, Olga N.; Keasling, Jay D.
- ACS Synthetic Biology, Vol. 4, Issue 4
Recovery of an ionic liquid [BMIM]Cl from a hydrolysate of lignocellulosic biomass using electrodialysis
journal, December 2013
- Trinh, Ly Thi Phi; Lee, Young Ju; Lee, Jae-Won
- Separation and Purification Technology, Vol. 120
Molecular weight distributions of polysaccharides and lignin extracted from plant biomass with a polar ionic liquid analysed without a derivatisation process
journal, January 2015
- Kuroda, Kosuke; Fukaya, Yukinobu; Yamada, Tatsuhiko
- Analytical Methods, Vol. 7, Issue 5
One-pot integrated biofuel production using low-cost biocompatible protic ionic liquids
journal, January 2017
- Sun, Jian; Konda, N. V. S. N. Murthy; Parthasarathi, Ramakrishnan
- Green Chemistry, Vol. 19, Issue 13
One-pot ionic liquid pretreatment and saccharification of switchgrass
journal, January 2013
- Shi, Jian; Gladden, John M.; Sathitsuksanoh, Noppadon
- Green Chemistry, Vol. 15, Issue 9
High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
journal, August 2007
- Li, Yonghong; Zhao, Zongbao (Kent); Bai, Fengwu
- Enzyme and Microbial Technology, Vol. 41, Issue 3
The influence of lactic acid formation on the simultaneous saccharification and fermentation (SSF) of softwood to ethanol
journal, January 2000
- Stenberg, Kerstin; Galbe, Mats; Zacchi, Guido
- Enzyme and Microbial Technology, Vol. 26, Issue 1
Extracellular oxidative systems of the lignin-degrading Basidiomycete Phanerochaete chrysosporium
journal, February 2007
- Kersten, Phil; Cullen, Dan
- Fungal Genetics and Biology, Vol. 44, Issue 2
Efficient dehydration and recovery of ionic liquid after lignocellulosic processing using pervaporation
journal, June 2017
- Sun, Jian; Shi, Jian; Murthy Konda, N. V. S. N.
- Biotechnology for Biofuels, Vol. 10, Issue 1
Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks
journal, February 2015
- Shi, Jian; George, Kevin W.; Sun, Ning
- BioEnergy Research, Vol. 8, Issue 3
Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle
journal, December 2010
- Shill, Kierston; Padmanabhan, Sasisanker; Xin, Qin
- Biotechnology and Bioengineering, Vol. 108, Issue 3, p. 511-520
Works referencing / citing this record:
Ionic Liquids in Biomass Processing
journal, December 2018
- Amarasekara, Ananda S.
- Israel Journal of Chemistry, Vol. 59, Issue 9
Pilot-scale hydrothermal pretreatment and optimized saccharification enables bisabolene production from multiple feedstocks
journal, January 2019
- Pérez Pimienta, José A.; Papa, Gabriella; Rodriguez, Alberto
- Green Chemistry, Vol. 21, Issue 11
Sustainable bioproduction of the blue pigment indigoidine: Expanding the range of heterologous products in R. toruloides to include non-ribosomal peptides
journal, January 2019
- Wehrs, Maren; Gladden, John M.; Liu, Yuzhong
- Green Chemistry, Vol. 21, Issue 12
Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC
journal, October 2018
- Eng, Thomas; Demling, Philipp; Herbert, Robin A.
- Microbial Cell Factories, Vol. 17, Issue 1
Microbial Platform for Terpenoid Production: Escherichia coli and Yeast
journal, October 2018
- Wang, Chonglong; Liwei, Mudanguli; Park, Ji-Bin
- Frontiers in Microbiology, Vol. 9
Figures / Tables found in this record: