In vivo transport of three radioactive [18F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1

Tran, Thu M.; Hampton, Carissa S.; Brossard, Tom W.; Harmata, Michael; Robertson, J. David; Jurisson, Silvia S.; Braun, David M.

doi:10.1016/j.plaphy.2017.03.006

Title: In vivo transport of three radioactive [¹⁸F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1

Journal Article · Wed Mar 08 00:00:00 EST 2017 · Plant Physiology and Biochemistry

DOI:https://doi.org/10.1016/j.plaphy.2017.03.006· OSTI ID:1418477

Tran, Thu M. ^[1]; Hampton, Carissa S. ^[2]; Brossard, Tom W. ^[2]; Harmata, Michael ^[3];

^[2]; Jurisson, Silvia S. ^[4]; Braun, David M. ^[1]

Univ. of Missouri, Columbia, MO (United States). Division of Biological Sciences, Interdisciplinary Plant Group, and Missouri Maize Center; Univ. of Missouri, Columbia, MO (United States). Plant Imaging Consortium
Univ. of Missouri, Columbia, MO (United States). Dept. of Chemistry; Univ. of Missouri, Columbia, MO (United States). Research Reactor
Univ. of Missouri, Columbia, MO (United States). Dept. of Chemistry
Univ. of Missouri, Columbia, MO (United States). Division of Biological Sciences, Interdisciplinary Plant Group, and Missouri Maize Center; Univ. of Missouri, Columbia, MO (United States). Dept. of Chemistry

Sucrose transporter (SUT) proteins translocate sucrose across cell membranes; however, mechanistic aspects of sucrose binding by SUTs are not well resolved. Specific hydroxyl groups in sucrose participate in hydrogen bonding with SUT proteins. We previously reported that substituting a radioactive fluorine-18 [¹⁸F] at the C-60 position within the fructosyl moiety of sucrose did not affect sucrose transport by the maize (Zea mays) ZmSUT1 protein. To determine how ¹⁸F substitution of hydroxyl groups at two other positions within sucrose, the C-10 in the fructosyl moiety or the C-6 in the glucosyl moiety, impact sucrose transport, we synthesized 10-[F¹⁸]fluoro-10-deoxysucrose and 6-[F¹⁸]fluoro-6-deoxysucrose ([¹⁸F] FDS) analogs. In this work, each [¹⁸F]FDS derivative was independently introduced into wild-type or sut1 mutant plants, which are defective in sucrose phloem loading. All three (10-, 60-, and 6-) [¹⁸F]FDS derivatives were efficiently and equally translocated, similarly to carbon-14 [¹⁴C]-labeled sucrose. Hence, individually replacing the hydroxyl groups at these positions within sucrose does not interfere with substrate recognition, binding, or membrane transport processes, and hydroxyl groups at these three positions are not essential for hydrogen bonding between sucrose and ZmSUT1. [¹⁸F]FDS imaging afforded several advantages compared to [¹⁴C]-sucrose detection. We calculated that 10-[¹⁸F]FDS was transported at approximately a rate of 0.90 ± 0.15 m.h-1 in wild-type leaves, and at 0.68 ± 0.25 m.h-1 in sut1 mutant leaves. Collectively, our data indicated that [¹⁸F]FDS analogs are valuable tools to probe sucrose-SUT interactions and to monitor sucrose transport in plants.

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Research Organization:: Univ. of Missouri, Columbia, MO (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; Vietnam International Education Department

Grant/Contract Number:: SC0002040; IIA-1430428; IOS-1025976

OSTI ID:: 1418477

Alternate ID(s):: OSTI ID: 1439728

Journal Information:: Plant Physiology and Biochemistry, Vol. 115, Issue C; ISSN 0981-9428

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 12 works

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References (91)

Identification and characterization of a sucrose transporter isolated from the developing cotyledons of soybean Aldape, Mike J.; Elmer, Aaron M.; Chao, Wun S. Archives of Biochemistry and Biophysics, Vol. 409, Issue 2 https://doi.org/10.1016/S0003-9861(02)00631-8	journal	January 2003
The Sucrose Transporter Gene Family in Rice Aoki, Naohiro; Hirose, Tatsuro; Scofield, Graham N. Plant and Cell Physiology, Vol. 44, Issue 3 https://doi.org/10.1093/pcp/pcg030	journal	March 2003
Molecular Cloning and Expression Analysis of a Gene for a Sucrose Transporter in Maize (Zea mays L.) Aoki, N.; Hirose, T.; Takahashi, S. Plant and Cell Physiology, Vol. 40, Issue 10 https://doi.org/10.1093/oxfordjournals.pcp.a029489	journal	January 1999
Membrane-Transport Systems for Sucrose in Relation to Whole-Plant Carbon Partitioning Ayre, Brian G. Molecular Plant, Vol. 4, Issue 3 https://doi.org/10.1093/mp/ssr014	journal	May 2011
tie - dyed1 Functions Non-Cell Autonomously to Control Carbohydrate Accumulation in Maize Leaves Baker, R. Frank; Braun, David M. Plant Physiology, Vol. 144, Issue 2 https://doi.org/10.1104/pp.107.098814	journal	April 2007
Tie-dyed2 Functions with Tie-dyed1 to Promote Carbohydrate Export from Maize Leaves Baker, R. Frank; Braun, David M. Plant Physiology, Vol. 146, Issue 3 https://doi.org/10.1104/pp.107.111476	journal	January 2008
Sucrose Transporter ZmSut1 Expression and Localization Uncover New Insights into Sucrose Phloem Loading Baker, R. Frank; Leach, Kristen A.; Boyer, Nathanial R. Plant Physiology, Vol. 172, Issue 3 https://doi.org/10.1104/pp.16.00884	journal	September 2016
SWEET as Sugar: New Sucrose Effluxers in Plants Frank Baker, R.; Leach, Kristen A.; Braun, David M. Molecular Plant, Vol. 5, Issue 4 https://doi.org/10.1093/mp/SSS054	journal	July 2012
An Analysis of Translocation in the Phloem of the Bean Plant Using Tho, P³², And C¹⁴. Biddulph, O.; Cory, R. Plant Physiology, Vol. 32, Issue 6 https://doi.org/10.1104/pp.32.6.608	journal	November 1957
Sucrose accumulation in sweet sorghum stems occurs by apoplasmic phloem unloading and does not involve differential Sucrose transporter expression Bihmidine, Saadia; Baker, R. Frank; Hoffner, Cassandra BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0572-8	journal	July 2015
Regulation of assimilate import into sink organs: update on molecular drivers of sink strength Bihmidine, Saadia; Hunter, Charles T.; Johns, Christine E. Frontiers in Plant Science, Vol. 4 https://doi.org/10.3389/fpls.2013.00177	journal	January 2013
Tonoplast Sugar Transporters (SbTSTs) putatively control sucrose accumulation in sweet sorghum stems Bihmidine, Saadia; Julius, Benjamin T.; Dweikat, Ismail Plant Signaling & Behavior, Vol. 11, Issue 1 https://doi.org/10.1080/15592324.2015.1117721	journal	January 2016
SWEET! The Pathway Is Complete Braun, D. M. Science, Vol. 335, Issue 6065 https://doi.org/10.1126/science.1216828	journal	January 2012
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading Braun, David M.; Slewinski, Thomas L. Plant Physiology, Vol. 149, Issue 1 https://doi.org/10.1104/pp.108.129049	journal	January 2009
Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security Braun, D. M.; Wang, L.; Ruan, Y. -L. Journal of Experimental Botany, Vol. 65, Issue 7 https://doi.org/10.1093/jxb/ert416	journal	December 2013
Chemoenzymic syntheses of fructose-modified sucroses via multienzyme systems. Some topographical aspects of the binding of sucrose to a sucrose carrier protein Card, Peter J.; Hitz, William D.; Ripp, Kevin G. Journal of the American Chemical Society, Vol. 108, Issue 1 https://doi.org/10.1021/ja00261a025	journal	January 1986
Phloem-localized, Proton-coupled Sucrose Carrier ZmSUT1 Mediates Sucrose Efflux under the Control of the Sucrose Gradient and the Proton Motive Force Carpaneto, Armando; Geiger, Dietmar; Bamberg, Ernst Journal of Biological Chemistry, Vol. 280, Issue 22 https://doi.org/10.1074/jbc.M501785200	journal	April 2005
Sucrose- and H+-Dependent Charge Movements Associated with the Gating of Sucrose Transporter ZmSUT1 Carpaneto, Armando; Koepsell, Hermann; Bamberg, Ernst PLoS ONE, Vol. 5, Issue 9 https://doi.org/10.1371/journal.pone.0012605	journal	September 2010
Substrate Specificity of the Arabidopsis thaliana Sucrose Transporter AtSUC2 Chandran, Divya; Reinders, Anke; Ward, John M. Journal of Biological Chemistry, Vol. 278, Issue 45 https://doi.org/10.1074/jbc.M308490200	journal	September 2003
Sucrose Efflux Mediated by SWEET Proteins as a Key Step for Phloem Transport Chen, L. -Q.; Qu, X. -Q.; Hou, B. -H. Science, Vol. 335, Issue 6065 https://doi.org/10.1126/science.1213351	journal	December 2011
Time-course Study of Translocation of Products of Photosynthesis in Soybean Plants Clauss, Heinz; Mortimer, D. C.; Gorham, P. R. Plant Physiology, Vol. 39, Issue 2 https://doi.org/10.1104/pp.39.2.269	journal	March 1964
Conformational Changes Represent the Rate-Limiting Step in the Transport Cycle of Maize SUCROSE TRANSPORTER1 Derrer, Carmen; Wittek, Anke; Bamberg, Ernst The Plant Cell, Vol. 25, Issue 8 https://doi.org/10.1105/tpc.113.113621	journal	August 2013
Organic Fluorine Hardly Ever Accepts Hydrogen Bonds Dunitz, Jack D.; Taylor, Robin Chemistry - A European Journal, Vol. 3, Issue 1 https://doi.org/10.1002/chem.19970030115	journal	January 1997
SWEETs, transporters for intracellular and intercellular sugar translocation Eom, Joon-Seob; Chen, Li-Qing; Sosso, Davide Current Opinion in Plant Biology, Vol. 25 https://doi.org/10.1016/j.pbi.2015.04.005	journal	June 2015
Leaf structure in relation to solute transport and phloem loading in Zea mays L. Evert, R. F.; Eschrich, W.; Heyser, W. Planta, Vol. 138, Issue 3 https://doi.org/10.1007/BF00386823	journal	January 1978
Applications of 2-deoxy-2-fluoro-D-glucose (FDG) in Plant Imaging: Past, Present, and Future Fatangare, Amol; Svatoš, Aleš Frontiers in Plant Science, Vol. 7 https://doi.org/10.3389/fpls.2016.00483	journal	May 2016
Novel application of 2-[18F]fluoro-2-deoxy-d-glucose to study plant defenses Ferrieri, Abigail P.; Appel, Heidi; Ferrieri, Richard A. Nuclear Medicine and Biology, Vol. 39, Issue 8 https://doi.org/10.1016/j.nucmedbio.2012.06.005	journal	November 2012
Future Scenarios for Plant Phenotyping Fiorani, Fabio; Schurr, Ulrich Annual Review of Plant Biology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev-arplant-050312-120137	journal	April 2013
Kinetics of C-14 Translocation in Soybean Fisher, Donald B. Plant Physiology, Vol. 45, Issue 2 https://doi.org/10.1104/pp.45.2.119	journal	February 1970
Novel imaging-based phenotyping strategies for dissecting crosstalk in plant development Fraas, Simon; Lüthen, Hartwig Journal of Experimental Botany, Vol. 66, Issue 16 https://doi.org/10.1093/jxb/erv265	journal	June 2015
Loading and transport of assimilates in different maize leaf bundles Fritz, Eberhard; Evert, Ray F.; Nasse, Hubert Planta, Vol. 178, Issue 1 https://doi.org/10.1007/BF00392520	journal	January 1989
Synthesis of 6′-deoxy-6′-fluorosucrose Gaddam, Vikram; Harmata, Michael Carbohydrate Research, Vol. 369 https://doi.org/10.1016/j.carres.2012.12.001	journal	March 2013
A synthesis of 6-deoxy-6-fluorosucrose suitable for PET applications Gao, Xuefeng; Gaddam, Vikram; Harmata, Michael Carbohydrate Research, Vol. 400 https://doi.org/10.1016/j.carres.2014.08.015	journal	December 2014
Plant Sucrose Transporters from a Biophysical Point of View Geiger, Dietmar Molecular Plant, Vol. 4, Issue 3 https://doi.org/10.1093/mp/ssr029	journal	May 2011
Translocation and Accumulation of Translocate in the Sugar Beet Petiole Geiger, D. R.; Saunders, M. A.; Cataldo, D. A. Plant Physiology, Vol. 44, Issue 12 https://doi.org/10.1104/pp.44.12.1657	journal	December 1969
Radiosynthesis of the fluorinated sucrose analogue, 1′-[ ¹⁸ F]fluoro-1′-deoxysucrose Gifford, Andrew N.; Bennett, Caitlin V.; Fowler, Joanna S. Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 55, Issue 12 https://doi.org/10.1002/jlcr.2969	journal	October 2012
The α-d-glucosyl C-2 hydroxyl is required for binding to the H+-sucrose transporter in phloem Griffin, Simon D. J.; Buxton, Karen D.; Donaldson, Iain A. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1152, Issue 1 https://doi.org/10.1016/0005-2736(93)90231-N	journal	October 1993
6(5)Carboxyfluorescein as a Tracer of Phloem sap Translocation Grignon, Nicole; Touraine, Bruno; Durand, Monique American Journal of Botany, Vol. 76, Issue 6 https://doi.org/10.1002/j.1537-2197.1989.tb15064.x	journal	June 1989
Translocation of ¹⁴C in the Sugarcane Plant During the Day and Night Hartt, Constance E.; Kortschak, Hugo P. Plant Physiology, Vol. 42, Issue 1 https://doi.org/10.1104/pp.42.1.89	journal	January 1967
Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system Hattori, Etsuko; Uchida, Hiroshi; Harada, Norihiro Planta, Vol. 227, Issue 5 https://doi.org/10.1007/s00425-008-0701-9	journal	February 2008
Substrate Specificity of the H⁺-Sucrose Symporter on the Plasma Membrane of Sugar Beets (Beta vulgaris L.) Hecht, Roland; Slone, J. Henry; Buckhout, Thomas J. Plant Physiology, Vol. 99, Issue 2 https://doi.org/10.1104/pp.99.2.439	journal	June 1992
Sucrose in the Free Space of Translocating Maize Leaf Bundles Heyser, Wolfgang; Evert, Ray F.; Fritz, Eberhard Plant Physiology, Vol. 62, Issue 4 https://doi.org/10.1104/pp.62.4.491	journal	October 1978
Substrate recognition by a sucrose transporting protein. Hitz, W. D.; Card, P. J.; Ripp, K. G. Journal of Biological Chemistry, Vol. 261, Issue 26 https://doi.org/10.1016/S0021-9258(18)67191-1	journal	September 1986
Transport and Metabolism of 1′-Fluorosucrose, a Sucrose Analog Not Subject to Invertase Hydrolysis Hitz, William D.; Schmitt, Mark R.; Card, Peter J. Plant Physiology, Vol. 77, Issue 2 https://doi.org/10.1104/pp.77.2.291	journal	February 1985
The translocation of photosynthetically assimilated ¹⁴ C in corn Hofstra, G.; Nelson, C. D. Canadian Journal of Botany, Vol. 47, Issue 9 https://doi.org/10.1139/b69-205	journal	September 1969
Plant-PET Scans: In Vivo Mapping of Xylem and Phloem Functioning Hubeau, Michiel; Steppe, Kathy Trends in Plant Science, Vol. 20, Issue 10 https://doi.org/10.1016/j.tplants.2015.07.008	journal	October 2015
Labeled 2-deoxy-D-glucose analogs. 18F-labeled 2-deoxy-2-fluoro-D-glucose, 2-deoxy-2-fluoro-D-mannose and 14C-2-deoxy-2-fluoro-D-glucose Ido, T.; Wan, C-N.; Casella, V. Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 14, Issue 2 https://doi.org/10.1002/jlcr.2580140204	journal	January 1978
Diurnal Changes in Maize Leaf Photosynthesis: I. Carbon Exchange Rate, Assimilate Export Rate, and Enzyme Activities Kalt-Torres, Willy; Kerr, Phillip S.; Usuda, Hideaki Plant Physiology, Vol. 83, Issue 2 https://doi.org/10.1104/pp.83.2.283	journal	February 1987
In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner Karve, Abhijit A.; Alexoff, David; Kim, Dohyun BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0658-3	journal	November 2015
Real-time whole-plant imaging of 11C translocation using positron-emitting tracer imaging system Kawachi, Naoki; Suzui, Nobuo; Ishii, Satomi Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 648 https://doi.org/10.1016/j.nima.2010.10.152	journal	August 2011
Testing the Münch hypothesis of long distance phloem transport in plants Knoblauch, Michael; Knoblauch, Jan; Mullendore, Daniel L. eLife, Vol. 5 https://doi.org/10.7554/eLife.15341	journal	June 2016
Multispectral Phloem-Mobile Probes: Properties and Applications Knoblauch, Michael; Vendrell, Marc; de Leau, Erica Plant Physiology, Vol. 167, Issue 4 https://doi.org/10.1104/pp.114.255414	journal	February 2015
Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development Koch, Karen Current Opinion in Plant Biology, Vol. 7, Issue 3 https://doi.org/10.1016/j.pbi.2004.03.014	journal	June 2004
Sucrose transporter2 contributes to maize growth, development, and crop yield: ZmSUT2 contributes to plant growth and yield Leach, Kristen A.; Tran, Thu M.; Slewinski, Thomas L. Journal of Integrative Plant Biology, Vol. 59, Issue 6 https://doi.org/10.1111/jipb.12527	journal	April 2017
Sugar Transport in Isolated Corn Root Protoplasts Lin, Willy; Schmitt, Mark R.; Hitz, William D. Plant Physiology, Vol. 76, Issue 4 https://doi.org/10.1104/pp.76.4.894	journal	December 1984
Transport of sucrose, not hexose, in the phloem Liu, David D.; Chao, Wesley M.; Turgeon, Robert Journal of Experimental Botany, Vol. 63, Issue 11 https://doi.org/10.1093/jxb/ers127	journal	May 2012
Is the infiltration-centrifugation technique appropriate for the isolation of apoplastic fluid? A critical evaluation with different plant species Lohaus, Gertrud; Pennewiss, Kerstin; Sattelmacher, Burkhard Physiologia Plantarum, Vol. 111, Issue 4 https://doi.org/10.1034/j.1399-3054.2001.1110405.x	journal	April 2001
Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves Ma, Yi; Baker, R. Frank; Magallanes-Lundback, Maria Planta, Vol. 227, Issue 3 https://doi.org/10.1007/s00425-007-0636-6	journal	October 2007
Control of photoassimilate movement in source-leaf tissues of Ipomoea tricolor Cav. Madore, Monica A.; Lucas, William J. Planta, Vol. 171, Issue 2 https://doi.org/10.1007/BF00391094	journal	June 1987
The partitioning of ¹⁴ C between growth and differentiation within stem-deformed and healthy black spruce seedlings Margolis, Hank A.; Delaney, Sandra; Vézina, Louis-P. Canadian Journal of Botany, Vol. 69, Issue 6 https://doi.org/10.1139/b91-157	journal	June 1991
Molecular imaging in living subjects: seeing fundamental biological processes in a new light Massoud, T. F. Genes & Development, Vol. 17, Issue 5 https://doi.org/10.1101/gad.1047403	journal	March 2003
A new visualization technique for the study of the accumulation of photoassimilates in wheat grains using [11C]CO2 Matsuhashi, Shinpei; Fujimaki, Shu; Uchida, Hiroshi Applied Radiation and Isotopes, Vol. 64, Issue 4 https://doi.org/10.1016/j.apradiso.2005.08.020	journal	April 2006
Using the short-lived isotope 11C in mechanistic studies of photosynthate transport Minchin, Peter E. H.; Thorpe, Michael R. Functional Plant Biology, Vol. 30, Issue 8 https://doi.org/10.1071/FP03008	journal	January 2003
The Translocation of¹¹C-labelled Photosynthate in the Soybean Moorby, J.; Ebert, M.; Evans, N. T. S. Journal of Experimental Botany, Vol. 14, Issue 2 https://doi.org/10.1093/jxb/14.2.210	journal	January 1963
Application of positron emission tomography and 2-[18F]fluoro-2-deoxy-d-glucose for visualization and quantification of solute transport in plant tissues Partelová, Denisa; Uhrovčík, Jozef; Lesný, Juraj Chemical Papers, Vol. 68, Issue 11 https://doi.org/10.2478/s11696-014-0609-8	journal	January 2014
Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose Reinders, Anke; Sivitz, Alicia B.; Hsi, Alex Plant, Cell and Environment, Vol. 29, Issue 10 https://doi.org/10.1111/j.1365-3040.2006.01563.x	journal	October 2006
Evolution of plant sucrose uptake transporters Reinders, Anke Frontiers in Plant Science, Vol. 3 https://doi.org/10.3389/fpls.2012.00022	journal	January 2012
Radiosynthesis of 6’-Deoxy-6’[18F]Fluorosucrose via Automated Synthesis and Its Utility to Study In Vivo Sucrose Transport in Maize (Zea mays) Leaves Rotsch, David; Brossard, Tom; Bihmidine, Saadia PLOS ONE, Vol. 10, Issue 5 https://doi.org/10.1371/journal.pone.0128989	journal	May 2015
Sucrose Metabolism: Gateway to Diverse Carbon Use and Sugar Signaling Ruan, Yong-Ling Annual Review of Plant Biology, Vol. 65, Issue 1 https://doi.org/10.1146/annurev-arplant-050213-040251	journal	April 2014
Leaf vasculature in Zea mays L. Russell, S. H.; Evert, R. F. Planta, Vol. 164, Issue 4 https://doi.org/10.1007/BF00395960	journal	January 1985
Transport and Metabolism of a Sucrose Analog (1′-Fluorosucrose) into Zea mays L. Endosperm without Invertase Hydrolysis Schmalstig, Judy Gougler; Hitz, William D. Plant Physiology, Vol. 85, Issue 4 https://doi.org/10.1104/pp.85.4.902	journal	December 1987
Sugar Transport into Protoplasts Isolated from Developing Soybean Cotyledons Schmitt, Mark R.; Hitz, William D.; Lin, Willy Plant Physiology, Vol. 75, Issue 4 https://doi.org/10.1104/pp.75.4.941	journal	August 1984
Analysis of the Transport Activity of Barley Sucrose Transporter HvSUT1 Sivitz, Alicia B.; Reinders, Anke; Ward, John M. Plant and Cell Physiology, Vol. 46, Issue 10 https://doi.org/10.1093/pcp/pci182	journal	October 2005
Tie-dyed2 Encodes a Callose Synthase That Functions in Vein Development and Affects Symplastic Trafficking within the Phloem of Maize Leaves Slewinski, Thomas L.; Baker, R. Frank; Stubert, Adam Plant Physiology, Vol. 160, Issue 3 https://doi.org/10.1104/pp.112.202473	journal	August 2012
Current perspectives on the regulation of whole-plant carbohydrate partitioning Slewinski, Thomas L.; Braun, David M. Plant Science, Vol. 178, Issue 4 https://doi.org/10.1016/j.plantsci.2010.01.010	journal	April 2010
The Psychedelic Genes of Maize Redundantly Promote Carbohydrate Export From Leaves Slewinski, Thomas L.; Braun, David M. Genetics, Vol. 185, Issue 1 https://doi.org/10.1534/genetics.109.113357	journal	February 2010
Maize SUT1 functions in phloem loading Slewinski, Thomas L.; Garg, Anshu; Johal, Gurmukh S. Plant Signaling & Behavior, Vol. 5, Issue 6 https://doi.org/10.4161/psb.5.6.11575	journal	June 2010
Sucrose transporter1 functions in phloem loading in maize leaves Slewinski, Thomas L.; Meeley, Robert; Braun, David M. Journal of Experimental Botany, Vol. 60, Issue 3 https://doi.org/10.1093/jxb/ern335	journal	January 2009
Functionally Important Amino Acids in Rice Sucrose Transporter OsSUT1 Sun, Ye; Lin, Zi; Reinders, Anke Biochemistry, Vol. 51, Issue 15 https://doi.org/10.1021/bi201934h	journal	April 2012
Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5 Sun, Y.; Reinders, A.; LaFleur, K. R. Plant and Cell Physiology, Vol. 51, Issue 1 https://doi.org/10.1093/pcp/pcp172	journal	December 2009
Arg188 in rice sucrose transporter OsSUT1 is crucial for substrate transport Sun, Ye; Ward, John M. BMC Biochemistry, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2091-13-26	journal	January 2012
Use of positron-emitting tracer imaging system for measuring the effect of salinity on temporal and spatial distribution of 11C tracer and coupling between source and sink organs Suwa, Ryuichi; Fujimaki, Shu; Suzui, Nobuo Plant Science, Vol. 175, Issue 3 https://doi.org/10.1016/j.plantsci.2008.03.022	journal	September 2008
11C-imaging: methyl jasmonate moves in both phloem and xylem, promotes transport of jasmonate, and of photoassimilate even after proton transport is decoupled Thorpe, Michael R.; Ferrieri, Abigail P.; Herth, Matthias M. Planta, Vol. 226, Issue 2 https://doi.org/10.1007/s00425-007-0503-5	journal	March 2007
Photoassimilate partitioning in nodulated soybean I. 11C methodology Thorpe, M. R.; Walsh, K. B.; Minchin, P. E. H. Journal of Experimental Botany, Vol. 49, Issue 328 https://doi.org/10.1093/jxb/49.328.1805	journal	November 1998
Relations between Light Level, Sucrose Concentration, and Translocation of Carbon 11 in Zea mays Leaves Troughton, John H.; Currie, B. G.; Chang, F. H. Plant Physiology, Vol. 59, Issue 5 https://doi.org/10.1104/pp.59.5.808	journal	May 1977
The Puzzle of Phloem Pressure Turgeon, Robert Plant Physiology, Vol. 154, Issue 2 https://doi.org/10.1104/pp.110.161679	journal	October 2010
Phloem Transport: Cellular Pathways and Molecular Trafficking Turgeon, Robert; Wolf, Shmuel Annual Review of Plant Biology, Vol. 60, Issue 1 https://doi.org/10.1146/annurev.arplant.043008.092045	journal	June 2009
The phloem, a miracle of ingenuity Van Bel, A. J. E. Plant, Cell and Environment, Vol. 26, Issue 1 https://doi.org/10.1046/j.1365-3040.2003.00963.x	journal	January 2003
Translocation of Photosynthetically Assimilated C¹⁴ in Straight-Necked Squash Webb, J. A.; Gorham, P. R. Plant Physiology, Vol. 39, Issue 4 https://doi.org/10.1104/pp.39.4.663	journal	July 1964
Transgenic approaches to altering carbon and nitrogen partitioning in whole plants: assessing the potential to improve crop yields and nutritional quality Yadav, Umesh P.; Ayre, Brian G.; Bush, Daniel R. Frontiers in Plant Science, Vol. 6 https://doi.org/10.3389/fpls.2015.00275	journal	April 2015
Chemical Synthesis of 1′-Deoxy-1′-fluorosucrose Ying, Weijiang; Gaddam, Vikram; Harmata, Michael Organic Letters, Vol. 15, Issue 11 https://doi.org/10.1021/ol401044h	journal	May 2013

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59 BASIC BIOLOGICAL SCIENCES
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ZmSUT1

Title: In vivo transport of three radioactive [18F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1

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Title: In vivo transport of three radioactive [¹⁸F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1