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Title: Ancient Regulatory Role of Lysine Acetylation in Central Metabolism

Abstract

ABSTRACT Lysine acetylation is a common protein post-translational modification in bacteria and eukaryotes. Unlike phosphorylation, whose functional role in signaling has been established, it is unclear what regulatory mechanism acetylation plays and whether it is conserved across evolution. By performing a proteomic analysis of 48 phylogenetically distant bacteria, we discovered conserved acetylation sites on catalytically essential lysine residues that are invariant throughout evolution. Lysine acetylation removes the residue’s charge and changes the shape of the pocket required for substrate or cofactor binding. Two-thirds of glycolytic and tricarboxylic acid (TCA) cycle enzymes are acetylated at these critical sites. Our data suggest that acetylation may play a direct role in metabolic regulation by switching off enzyme activity. We propose that protein acetylation is an ancient and widespread mechanism of protein activity regulation. IMPORTANCEPost-translational modifications can regulate the activity and localization of proteins inside the cell. Similar to phosphorylation, lysine acetylation is present in both eukaryotes and prokaryotes and modifies hundreds to thousands of proteins in cells. However, how lysine acetylation regulates protein function and whether such a mechanism is evolutionarily conserved is still poorly understood. Here, we investigated evolutionary and functional aspects of lysine acetylation by searching for acetylated lysines in amore » comprehensive proteomic data set from 48 phylogenetically distant bacteria. We found that lysine acetylation occurs in evolutionarily conserved lysine residues in catalytic sites of enzymes involved in central carbon metabolism. Moreover, this modification inhibits enzymatic activity. Our observations suggest that lysine acetylation is an evolutionarily conserved mechanism of controlling central metabolic activity by directly blocking enzyme active sites.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1439706
Report Number(s):
PNNL-SA-124565
Journal ID: ISSN 2150-7511; 48582; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
mBio (Online)
Additional Journal Information:
Journal Volume: 8; Journal Issue: 6; Journal ID: ISSN 2150-7511
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Nakayasu, Ernesto S., Burnet, Meagan C., Walukiewicz, Hanna E., Wilkins, Christopher S., Shukla, Anil K., Brooks, Shelby, Plutz, Matthew J., Lee, Brady D., Schilling, Birgit, Wolfe, Alan J., Müller, Susanne, Kirby, John R., Rao, Christopher V., Cort, John R., Payne, Samuel H., and Msadek, Tarek. Ancient Regulatory Role of Lysine Acetylation in Central Metabolism. United States: N. p., 2017. Web. doi:10.1128/mBio.01894-17.
Nakayasu, Ernesto S., Burnet, Meagan C., Walukiewicz, Hanna E., Wilkins, Christopher S., Shukla, Anil K., Brooks, Shelby, Plutz, Matthew J., Lee, Brady D., Schilling, Birgit, Wolfe, Alan J., Müller, Susanne, Kirby, John R., Rao, Christopher V., Cort, John R., Payne, Samuel H., & Msadek, Tarek. Ancient Regulatory Role of Lysine Acetylation in Central Metabolism. United States. doi:10.1128/mBio.01894-17.
Nakayasu, Ernesto S., Burnet, Meagan C., Walukiewicz, Hanna E., Wilkins, Christopher S., Shukla, Anil K., Brooks, Shelby, Plutz, Matthew J., Lee, Brady D., Schilling, Birgit, Wolfe, Alan J., Müller, Susanne, Kirby, John R., Rao, Christopher V., Cort, John R., Payne, Samuel H., and Msadek, Tarek. Tue . "Ancient Regulatory Role of Lysine Acetylation in Central Metabolism". United States. doi:10.1128/mBio.01894-17.
@article{osti_1439706,
title = {Ancient Regulatory Role of Lysine Acetylation in Central Metabolism},
author = {Nakayasu, Ernesto S. and Burnet, Meagan C. and Walukiewicz, Hanna E. and Wilkins, Christopher S. and Shukla, Anil K. and Brooks, Shelby and Plutz, Matthew J. and Lee, Brady D. and Schilling, Birgit and Wolfe, Alan J. and Müller, Susanne and Kirby, John R. and Rao, Christopher V. and Cort, John R. and Payne, Samuel H. and Msadek, Tarek},
abstractNote = {ABSTRACT Lysine acetylation is a common protein post-translational modification in bacteria and eukaryotes. Unlike phosphorylation, whose functional role in signaling has been established, it is unclear what regulatory mechanism acetylation plays and whether it is conserved across evolution. By performing a proteomic analysis of 48 phylogenetically distant bacteria, we discovered conserved acetylation sites on catalytically essential lysine residues that are invariant throughout evolution. Lysine acetylation removes the residue’s charge and changes the shape of the pocket required for substrate or cofactor binding. Two-thirds of glycolytic and tricarboxylic acid (TCA) cycle enzymes are acetylated at these critical sites. Our data suggest that acetylation may play a direct role in metabolic regulation by switching off enzyme activity. We propose that protein acetylation is an ancient and widespread mechanism of protein activity regulation. IMPORTANCEPost-translational modifications can regulate the activity and localization of proteins inside the cell. Similar to phosphorylation, lysine acetylation is present in both eukaryotes and prokaryotes and modifies hundreds to thousands of proteins in cells. However, how lysine acetylation regulates protein function and whether such a mechanism is evolutionarily conserved is still poorly understood. Here, we investigated evolutionary and functional aspects of lysine acetylation by searching for acetylated lysines in a comprehensive proteomic data set from 48 phylogenetically distant bacteria. We found that lysine acetylation occurs in evolutionarily conserved lysine residues in catalytic sites of enzymes involved in central carbon metabolism. Moreover, this modification inhibits enzymatic activity. Our observations suggest that lysine acetylation is an evolutionarily conserved mechanism of controlling central metabolic activity by directly blocking enzyme active sites.},
doi = {10.1128/mBio.01894-17},
journal = {mBio (Online)},
issn = {2150-7511},
number = 6,
volume = 8,
place = {United States},
year = {2017},
month = {11}
}