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Title: High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger

Abstract

In A. niger, two transcription factors, AraR and XlnR, regulate the production of enzymes involved in degradation of arabinoxylan and catabolism of the released L-arabinose and D-xylose. Deletion of both araR and xlnR in leads to reduced production of (hemi)cellulolytic enzymes and reduced growth on arabinan, arabinogalactan and xylan. In this study, we investigated the colonization and degradation of wheat bran by the A. niger reference strain CBS 137562 and araR/xlnR regulatory mutants using highresolution microscopy and exo-proteomics. We discovered that wheat bran flakes have a ‘rough’ and ‘smooth’ surface with substantially different affinity towards fungal hyphae. While colonization of the rough side was possible for all strains, the xlnR mutants struggled to survive on the smooth side of the wheat bran particles after 20 and 40 h post inoculation. Impaired colonization ability of the smooth surface of wheat bran was linked to reduced potential of DxlnR to secrete arabinoxylan and cellulosedegrading enzymes and indicates that XlnR is the major regulator that drives colonization of wheat bran in A. niger.

Authors:
 [1];  [1];  [2];  [2];  [2];  [2];  [2];  [2];  [1];  [1];  [1];  [2];  [1]
  1. Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands
  2. Department of Energy, Environmental Molecular Sciences Laboratory, Richland WA USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1439702
Report Number(s):
PNNL-SA-134692
Journal ID: ISSN 1462-2912; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 19; Journal Issue: 11; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Country of Publication:
United States
Language:
English

Citation Formats

Kowalczyk, Joanna E., Khosravi, Claire, Purvine, Samuel, Dohnalkova, Alice, Chrisler, William B., Orr, Galya, Robinson, Errol, Zink, Erika, Wiebenga, Ad, Peng, Mao, Battaglia, Evy, Baker, Scott, and de Vries, Ronald P. High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger. United States: N. p., 2017. Web. doi:10.1111/1462-2920.13923.
Kowalczyk, Joanna E., Khosravi, Claire, Purvine, Samuel, Dohnalkova, Alice, Chrisler, William B., Orr, Galya, Robinson, Errol, Zink, Erika, Wiebenga, Ad, Peng, Mao, Battaglia, Evy, Baker, Scott, & de Vries, Ronald P. High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger. United States. https://doi.org/10.1111/1462-2920.13923
Kowalczyk, Joanna E., Khosravi, Claire, Purvine, Samuel, Dohnalkova, Alice, Chrisler, William B., Orr, Galya, Robinson, Errol, Zink, Erika, Wiebenga, Ad, Peng, Mao, Battaglia, Evy, Baker, Scott, and de Vries, Ronald P. Fri . "High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger". United States. https://doi.org/10.1111/1462-2920.13923.
@article{osti_1439702,
title = {High resolution visualization and exo-proteomics reveal the physiological role of XlnR and AraR in plant biomass colonization and degradation by Aspergillus niger: Visualizing plant biomass degradation by A. niger},
author = {Kowalczyk, Joanna E. and Khosravi, Claire and Purvine, Samuel and Dohnalkova, Alice and Chrisler, William B. and Orr, Galya and Robinson, Errol and Zink, Erika and Wiebenga, Ad and Peng, Mao and Battaglia, Evy and Baker, Scott and de Vries, Ronald P.},
abstractNote = {In A. niger, two transcription factors, AraR and XlnR, regulate the production of enzymes involved in degradation of arabinoxylan and catabolism of the released L-arabinose and D-xylose. Deletion of both araR and xlnR in leads to reduced production of (hemi)cellulolytic enzymes and reduced growth on arabinan, arabinogalactan and xylan. In this study, we investigated the colonization and degradation of wheat bran by the A. niger reference strain CBS 137562 and araR/xlnR regulatory mutants using highresolution microscopy and exo-proteomics. We discovered that wheat bran flakes have a ‘rough’ and ‘smooth’ surface with substantially different affinity towards fungal hyphae. While colonization of the rough side was possible for all strains, the xlnR mutants struggled to survive on the smooth side of the wheat bran particles after 20 and 40 h post inoculation. Impaired colonization ability of the smooth surface of wheat bran was linked to reduced potential of DxlnR to secrete arabinoxylan and cellulosedegrading enzymes and indicates that XlnR is the major regulator that drives colonization of wheat bran in A. niger.},
doi = {10.1111/1462-2920.13923},
url = {https://www.osti.gov/biblio/1439702}, journal = {Environmental Microbiology},
issn = {1462-2912},
number = 11,
volume = 19,
place = {United States},
year = {2017},
month = {10}
}

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