Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae

Bailey, Daniel C.; Alexander, Evan; Rice, Matthew R.; Drake, Eric J.; Mydy, Lisa S.; Aldrich, Courtney C.; Gulick, Andrew M.

doi:10.1074/jbc.RA118.002798

Title: Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae

Journal Article · Wed Apr 04 00:00:00 EDT 2018 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.RA118.002798· OSTI ID:1439640

Bailey, Daniel C. ^[1]; Alexander, Evan ^[2]; Rice, Matthew R. ^[3]; Drake, Eric J. ^[1]; Mydy, Lisa S. ^[1]; Aldrich, Courtney C. ^[2];

^[1]

State Univ. of New York (SUNY), Buffalo, NY (United States); Hauptman-Woodward Medical Research Inst., Buffalo, NY (United States)
Univ. of Minnesota, Minneapolis, MN (United States)
Hauptman-Woodward Medical Research Inst., Buffalo, NY (United States)

Aerobactin, a citryl-hydroxamate siderophore, is produced by a number of pathogenic Gram-negative bacteria to aid in iron assimilation. Interest in this well-known siderophore was reignited by recent investigations suggesting that it plays a key role in mediating the enhanced virulence of a hypervirulent pathotype of Klebsiella pneumoniae (hvKP). In contrast to classical opportunistic strains of K. pneumoniae, hvKP causes serious life-threatening infections in previously healthy individuals in the community. Multiple contemporary reports have confirmed fears that the convergence of multidrug-resistant and hvKP pathotypes has led to the evolution of a highly transmissible, drug-resistant, and virulent “super bug.” Despite hvKP harboring four distinct siderophore operons, knocking out production of only aerobactin led to a significant attenuation of virulence. Herein, we continue our structural and functional studies on the biosynthesis of this crucial virulence factor. In vivo heterologous production and in vitro reconstitution of aerobactin biosynthesis from hvKP was carried out in this work, demonstrating the specificity, stereoselectivity, and kinetic throughput of the complete pathway. Additionally, we present a steady-state kinetic analysis and the X-ray crystal structure of the second aerobactin synthetase IucC, as well as describe a surface entropy reduction strategy that was employed for structure determination. Finally, we show solution X-ray scattering data that support a unique dimeric quaternary structure for IucC. These new insights into aerobactin assembly will help inform potential antivirulence strategies and advance our understanding of siderophore biosynthesis.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Institutes of Health (NIH); The Buffalo Clinical and Translational Research Center; Richard W. and Mae Stone Goode Foundation; National Cancer Institute (NCI); National Institute of General Medical Sciences (NIGMS); USDOE

Grant/Contract Number:: AI116998; UL1TR001412; ACB-12002; AGM-12006; AC02-06CH11357; 1S10OD012289-01A1; T32-AI007614

OSTI ID:: 1439640

Journal Information:: Journal of Biological Chemistry, Vol. 293, Issue 20; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 26 works

Citation information provided by
Web of Science

References (69)

“Nightmare” Bacteria on the Rise in US Hospitals, Long-term Care Facilities Kuehn, Bridget M. JAMA, Vol. 309, Issue 15 https://doi.org/10.1001/jama.2013.2922	journal	April 2013
Necrotizing fasciitis and psoas abscess caused by hypervirulent Klebsiella pneumoniae Jin, Su Sin; Kim, Youn Jeong; Kim, Sang Il Korean Journal of Medicine, Vol. 85, Issue 5 https://doi.org/10.3904/kjm.2013.85.5.540	journal	January 2013
AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis Schmelz, Stefan; Kadi, Nadia; McMahon, Stephen A. Nature Chemical Biology, Vol. 5, Issue 3 https://doi.org/10.1038/nchembio.145	journal	February 2009
Analysis of a DtxR-Regulated Iron Transport and Siderophore Biosynthesis Gene Cluster in Corynebacterium diphtheriae Kunkle, C. A.; Schmitt, M. P. Journal of Bacteriology, Vol. 187, Issue 2 https://doi.org/10.1128/JB.187.2.422-433.2005	journal	January 2005
Protein interface classification by evolutionary analysis Duarte, Jose M.; Srebniak, Adam; Schärer, Martin A. BMC Bioinformatics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2105-13-334	journal	January 2012
Primary osteomyelitis caused by hypervirulent Klebsiella pneumoniae Prokesch, Bonnie C.; TeKippe, Michael; Kim, Jiwoong The Lancet Infectious Diseases, Vol. 16, Issue 9 https://doi.org/10.1016/S1473-3099(16)30021-4	journal	September 2016
Yersiniabactin Synthetase Miller, Deborah Ann; Luo, Lusong; Hillson, Nathan Chemistry & Biology, Vol. 9, Issue 3 https://doi.org/10.1016/S1074-5521(02)00115-1	journal	March 2002
Aerobactin production by a planktonic marine Vibrio sp Haygood, Margo G.; Holt, Pamela D.; Butler, Alison Limnology and Oceanography, Vol. 38, Issue 5 https://doi.org/10.4319/lo.1993.38.5.1091	journal	July 1993
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1 https://doi.org/10.1107/S090744490705024X	journal	December 2007
Optimization of protein purification and characterization using Thermofluor screens Boivin, Stephane; Kozak, Sandra; Meijers, Rob Protein Expression and Purification, Vol. 91, Issue 2 https://doi.org/10.1016/j.pep.2013.08.002	journal	October 2013
Aerobactin, but Not Yersiniabactin, Salmochelin, or Enterobactin, Enables the Growth/Survival of Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae Ex Vivo and In Vivo Russo, Thomas A.; Olson, Ruth; MacDonald, Ulrike Infection and Immunity, Vol. 83, Issue 8 https://doi.org/10.1128/IAI.00430-15	journal	June 2015
Hypervirulent K. Pneumoniae Secretes More and More Active Iron-Acquisition Molecules than “Classical” K. Pneumoniae Thereby Enhancing its Virulence Russo, Thomas A.; Shon, Alyssa S.; Beanan, Janet M. PLoS ONE, Vol. 6, Issue 10 https://doi.org/10.1371/journal.pone.0026734	journal	October 2011
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-1 Gibson, F.; Magrath, D. I. Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 192, Issue 2 https://doi.org/10.1016/0304-4165(69)90353-5	journal	November 1969
A Continuous Spectrophotometric Assay for the Aminoacylation of Transfer RNA by Alanyl-Transfer RNA Synthetase Wu, M. X.; Hill, K. A. W. Analytical Biochemistry, Vol. 211, Issue 2 https://doi.org/10.1006/abio.1993.1276	journal	June 1993
Hypervirulent Klebsiella pneumoniae Patel, P. K.; Russo, T. A.; Karchmer, A. W. Open Forum Infectious Diseases, Vol. 1, Issue 1 https://doi.org/10.1093/ofid/ofu028	journal	March 2014
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
A New Method for the Synthesis ofN.epsilon.-Acetyl-N.epsilon.-hydroxy-L-lysine, the Iron-Binding Constituent of Several Important Siderophores Hu, Jingdan; Miller, Marvin J. The Journal of Organic Chemistry, Vol. 59, Issue 17 https://doi.org/10.1021/jo00096a030	journal	August 1994
Bacterial Meningitis Caused by Hypervirulent <i>Klebsiella pneumoniae</i> Capsular Genotype K54 with Development of Granuloma-like Nodal Enhancement in the Brain during the Subacute Phase Iwasaki, Yudai; Inokuchi, Ryota; Harada, Sohei Internal Medicine, Vol. 56, Issue 3 https://doi.org/10.2169/internalmedicine.56.7384	journal	January 2017
Isolation and properties of N.epsilon.-hydroxylysine:acetyl coenzyme A N.epsilon.-transacetylase from Escherichia coli pABN11 Coy, Mark; Paw, Barry H.; Bindereif, Albrecht Biochemistry, Vol. 25, Issue 9 https://doi.org/10.1021/bi00357a030	journal	May 1986
The design and synthesis of nucleoside triphosphate isosteres as potential inhibitors of HIV reverse transcriptase Weaver, Richard; Gilbert, Ian H. Tetrahedron, Vol. 53, Issue 15 https://doi.org/10.1016/S0040-4020(97)00210-X	journal	April 1997
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12. de Lorenzo, V.; Bindereif, A.; Paw, B. H. Journal of Bacteriology, Vol. 165, Issue 2 https://doi.org/10.1128/jb.165.2.570-578.1986	journal	February 1986
Photoreactivity of Iron(III)−Aerobactin: Photoproduct Structure and Iron(III) Coordination Küpper, Frithjof C.; Carrano, Carl J.; Kuhn, Jens-Uwe Inorganic Chemistry, Vol. 45, Issue 15 https://doi.org/10.1021/ic0604967	journal	July 2006
Protein crystallization by surface entropy reduction: optimization of the SER strategy Cooper, David R.; Boczek, Tomasz; Grelewska, Katarzyna Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 5 https://doi.org/10.1107/S0907444907010931	journal	April 2007
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Universal chemical assay for the detection and determination of siderophores Schwyn, Bernhard; Neilands, J. B. Analytical Biochemistry, Vol. 160, Issue 1, p. 47-56 https://doi.org/10.1016/0003-2697(87)90612-9	journal	January 1987
Aerobactin Mediates Virulence and Accounts for Increased Siderophore Production under Iron-Limiting Conditions by Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae Russo, T. A.; Olson, R.; MacDonald, U. Infection and Immunity, Vol. 82, Issue 6 https://doi.org/10.1128/IAI.01667-13	journal	March 2014
A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study Gu, Danxia; Dong, Ning; Zheng, Zhiwei The Lancet Infectious Diseases, Vol. 18, Issue 1 https://doi.org/10.1016/S1473-3099(17)30489-9	journal	January 2018
Klebsiella pneumoniae Antimicrobial Drug Resistance, United States, 1998–2010 Sanchez, Guillermo V.; Master, Ronald N.; Clark, Richard B. Emerging Infectious Diseases, Vol. 19, Issue 1 https://doi.org/10.3201/eid1901.120310	journal	January 2013
Klebsiella pneumoniae liver abscess in an immunocompetent child Kwon, Jang-Mi; Jung, Hye Lim; Shim, Jae Won Korean Journal of Pediatrics, Vol. 56, Issue 9 https://doi.org/10.3345/kjp.2013.56.9.407	journal	January 2013
The changing face of Klebsiella pneumoniae infections in the community Keynan, Yoav; Rubinstein, Ethan International Journal of Antimicrobial Agents, Vol. 30, Issue 5 https://doi.org/10.1016/j.ijantimicag.2007.06.019	journal	November 2007
Structural and Functional Characterization of Aerobactin Synthetase IucA from a Hypervirulent Pathotype of Klebsiella pneumoniae Bailey, Daniel C.; Drake, Eric J.; Grant, Thomas D. Biochemistry, Vol. 55, Issue 25 https://doi.org/10.1021/acs.biochem.6b00409	journal	June 2016
Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors Podschun, R.; Ullmann, U. Clinical Microbiology Reviews, Vol. 11, Issue 4 https://doi.org/10.1128/CMR.11.4.589	journal	October 1998
Resolution of a citric acid derivative: synthesis of (R)-(−)-homocitric acid-γ-lactone Ancliff, Rachael A.; Russell, Andrew T.; Sanderson, Adam J. Tetrahedron: Asymmetry, Vol. 8, Issue 20 https://doi.org/10.1016/S0957-4166(97)00435-7	journal	October 1997
Molecular pathogenesis of Klebsiella pneumoniae Li, Bei; Zhao, Yuling; Liu, Changting Future Microbiology, Vol. 9, Issue 9 https://doi.org/10.2217/fmb.14.48	journal	September 2014
Thermofluor-based high-throughput stability optimization of proteins for structural studies Ericsson, Ulrika B.; Hallberg, B. Martin; DeTitta, George T. Analytical Biochemistry, Vol. 357, Issue 2 https://doi.org/10.1016/j.ab.2006.07.027	journal	October 2006
How pathogenic bacteria evade mammalian sabotage in the battle for iron Fischbach, Michael A.; Lin, Hening; Liu, David R. Nature Chemical Biology, Vol. 2, Issue 3 https://doi.org/10.1038/nchembio771	journal	February 2006
New developments in the ATSAS program package for small-angle scattering data analysis Petoukhov, Maxim V.; Franke, Daniel; Shkumatov, Alexander V. Journal of Applied Crystallography, Vol. 45, Issue 2 https://doi.org/10.1107/S0021889812007662	journal	March 2012
Chemical Synthesis of Staphyloferrin B Affords Insight into the Molecular Structure, Iron Chelation, and Biological Activity of a Polycarboxylate Siderophore Deployed by the Human Pathogen Staphylococcus aureus Madsen, Julie L. H.; Johnstone, Timothy C.; Nolan, Elizabeth M. Journal of the American Chemical Society, Vol. 137, Issue 28 https://doi.org/10.1021/jacs.5b04557	journal	July 2015
The emergence of Klebsiella pneumoniae endogenous endophthalmitis in the USA: basic and clinical advances Kashani, Amir H.; Eliott, Dean Journal of Ophthalmic Inflammation and Infection, Vol. 3, Issue 1 https://doi.org/10.1186/1869-5760-3-28	journal	January 2013
Convergence of carbapenem-resistance and hypervirulence in Klebsiella pneumoniae Chen, Liang; Kreiswirth, Barry N. The Lancet Infectious Diseases, Vol. 18, Issue 1 https://doi.org/10.1016/S1473-3099(17)30517-0	journal	January 2018
Breaking a pathogen's iron will: Inhibiting siderophore production as an antimicrobial strategy Lamb, Audrey L. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 8 https://doi.org/10.1016/j.bbapap.2015.05.001	journal	August 2015
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222 Macheroux, Peter; Plattner, Hans Jurgen; Romaguera, Antonio European Journal of Biochemistry, Vol. 213, Issue 3 https://doi.org/10.1111/j.1432-1033.1993.tb17846.x	journal	May 1993
Trends in Resistance to Carbapenems and Third-Generation Cephalosporins among Clinical Isolates of Klebsiella pneumoniae in the United States, 1999–2010 Braykov, Nikolay P.; Eber, Michael R.; Klein, Eili Y. Infection Control & Hospital Epidemiology, Vol. 34, Issue 3 https://doi.org/10.1086/669523	journal	March 2013
Klebsiella pneumoniae Liver Abscess Associated With Septic Endophthalmitis Liu, Yung-Ching Archives of Internal Medicine, Vol. 146, Issue 10 https://doi.org/10.1001/archinte.1986.00360220057011	journal	October 1986
On a Boat: A Case in Australia of Endophthalmitis and Pyogenic Liver, Prostatic, and Lung Abscesses in a Previously Well Patient due to Klebsiella pneumoniae Vandevelde, Alecia; Stepanovic, Bojana Case Reports in Infectious Diseases, Vol. 2014 https://doi.org/10.1155/2014/137248	journal	January 2014
A Widely Distributed Bacterial Pathway for Siderophore Biosynthesis Independent of Nonribosomal Peptide Synthetases Challis, Gregory L. ChemBioChem, Vol. 6, Issue 4 https://doi.org/10.1002/cbic.200400283	journal	February 2005
Mapping the Evolution of Hypervirulent Klebsiella pneumoniae Struve, Carsten; Roe, Chandler C.; Stegger, Marc mBio, Vol. 6, Issue 4 https://doi.org/10.1128/mBio.00630-15	journal	July 2015
In Vitro Reconstitution of the Pseudomonas aeruginosa Nonribosomal Peptide Synthesis of Pyochelin: Characterization of Backbone Tailoring Thiazoline Reductase and N -Methyltransferase Activities ^† Patel, Hiten M.; Walsh, Christopher T. Biochemistry, Vol. 40, Issue 30 https://doi.org/10.1021/bi010519n	journal	July 2001
Biological small-angle X-ray scattering facility at the Stanford Synchrotron Radiation Laboratory Smolsky, Igor L.; Liu, Ping; Niebuhr, Marc Journal of Applied Crystallography, Vol. 40, Issue s1 https://doi.org/10.1107/S0021889807009624	journal	April 2007
Hypervirulent (hypermucoviscous) Klebsiella pneumoniae : A new and dangerous breed Shon, Alyssa S.; Bajwa, Rajinder P. S.; Russo, Thomas A. Virulence, Vol. 4, Issue 2 https://doi.org/10.4161/viru.22718	journal	February 2013
Purification and Structural Characterization of Siderophore (Corynebactin) from Corynebacterium diphtheriae Zajdowicz, Sheryl; Haller, Jon C.; Krafft, Amy E. PLoS ONE, Vol. 7, Issue 4 https://doi.org/10.1371/journal.pone.0034591	journal	April 2012
An integrated high-throughput data acquisition system for biological solution X-ray scattering studies Martel, Anne; Liu, Ping; Weiss, Thomas M. Journal of Synchrotron Radiation, Vol. 19, Issue 3 https://doi.org/10.1107/S0909049512008072	journal	March 2012
Necrotizing Fasciitis Caused by Hypermucoviscous Klebsiella pneumoniae in a Filipino Female in North America Ng, Daniel; Frazee, Brad Western Journal of Emergency Medicine, Vol. 16, Issue 1 https://doi.org/10.5811/westjem.2014.11.23599	journal	January 2015
Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli. de Lorenzo, V.; Neilands, J. B. Journal of Bacteriology, Vol. 167, Issue 1 https://doi.org/10.1128/jb.167.1.350-355.1986	journal	July 1986
Hypervirulent Klebsiella pneumoniae: the next superbug? Shon, Alyssa S.; Russo, Thomas A. Future Microbiology, Vol. 7, Issue 6 https://doi.org/10.2217/fmb.12.43	journal	June 2012
A cluster of five genes specifying the aerobactin iron uptake system of plasmid ColV-K30. Carbonetti, N. H.; Williams, P. H. Infection and Immunity, Vol. 46, Issue 1 https://doi.org/10.1128/IAI.46.1.7-12.1984	journal	January 1984
The accurate assessment of small-angle X-ray scattering data Grant, Thomas D.; Luft, Joseph R.; Carter, Lester G. Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 1 https://doi.org/10.1107/S1399004714010876	journal	January 2015
Novel Cord-like Structures on MRI in a Case of Hypervirulent <i>Klebsiella pneumoniae</i> Takahashi, Keiji; Miura, Atsushi; Yamaguchi, Tetsuo Internal Medicine, Vol. 54, Issue 3 https://doi.org/10.2169/internalmedicine.54.3485	journal	January 2015
Enantioselective desymmetrisation of citric acid catalysed by the substrate-tolerant petrobactin biosynthetic enzyme AsbA Oves-Costales, Daniel; Song, Lijiang; Challis, Gregory L. Chemical Communications, Issue 11 https://doi.org/10.1039/b823147h	journal	January 2009
A deliberate approach to screening for initial crystallization conditions of biological macromolecules Luft, Joseph R.; Collins, Robert J.; Fehrman, Nancy A. Journal of Structural Biology, Vol. 142, Issue 1 https://doi.org/10.1016/S1047-8477(03)00048-0	journal	April 2003
Structural Basis for Acyl Acceptor Specificity in the Achromobactin Biosynthetic Enzyme AcsD Schmelz, Stefan; Botting, Catherine H.; Song, Lijiang Journal of Molecular Biology, Vol. 412, Issue 3 https://doi.org/10.1016/j.jmb.2011.07.059	journal	September 2011
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
The long-overlooked enzymology of a nonribosomal peptide synthetase-independent pathway for virulence-conferring siderophore biosynthesis Oves-Costales, Daniel; Kadi, Nadia; Challis, Gregory L. Chemical Communications, Issue 43 https://doi.org/10.1039/b913092f	journal	January 2009
Genetic diversity and co-prevalence of ESBLs and PMQR genes among plasmid-mediated AmpC β-lactamase-producing Klebsiella pneumoniae isolates causing urinary tract infection Xiong, Yilin; Zhang, Cong; Gao, Wenting The Journal of Antibiotics, Vol. 74, Issue 6 https://doi.org/10.1038/s41429-021-00413-6	journal	March 2021
An Assessment of Siderophore Production, Mucoviscosity, and Mouse Infection Models for Defining the Virulence Spectrum of Hypervirulent Klebsiella pneumoniae Russo, Thomas A.; MacDonald, Ulrike; Hassan, Sidra mSphere, Vol. 6, Issue 2 https://doi.org/10.1128/msphere.00045-21	journal	April 2021
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard. Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. International Union of Crystallography (IUCr) https://doi.org/10.17863/cam.52329	journalarticle	January 2008
Protein interface classification by evolutionary analysis Duarte, Jose M.; Srebniak, Adam; Schärer, Martin A. ETH Zurich https://doi.org/10.3929/ethz-b-000063990	text	January 2012
Klebsiella pneumoniae Liver Abscess Associated With Septic Endophthalmitis Casanova, Ciro Archives of Internal Medicine, Vol. 149, Issue 6 https://doi.org/10.1001/archinte.1989.00390060171048	journal	June 1989
A Widely Distributed Bacterial Pathway for Siderophore Biosynthesis Independent of Nonribosomal Peptide Synthetases Challis, Gregory L. ChemBioChem, Vol. 8, Issue 13 https://doi.org/10.1002/cbic.200790044	journal	September 2007

Cited By (4)

Development of a High-Throughput Biochemical Assay to Screen for Inhibitors of Aerobactin Synthetase IucA Bailey, Daniel C.; Buckley, Brian P.; Chernov, Mikhail V. SLAS DISCOVERY: Advancing the Science of Drug Discovery, Vol. 23, Issue 10 https://doi.org/10.1177/2472555218787140	journal	July 2018
Chemistry and Biology of Siderophores from Marine Microbes Chen, Jianwei; Guo, Yuqi; Lu, Yaojia Marine Drugs, Vol. 17, Issue 10 https://doi.org/10.3390/md17100562	journal	September 2019
Targeting adenylate-forming enzymes with designed sulfonyladenosine inhibitors Lux, Michaelyn C.; Standke, Lisa C.; Tan, Derek S. The Journal of Antibiotics, Vol. 72, Issue 6 https://doi.org/10.1038/s41429-019-0171-2	journal	April 2019
Hypervirulent Klebsiella pneumoniae – clinical and molecular perspectives Choby, J. E.; Howard‐Anderson, J.; Weiss, D. S. Journal of Internal Medicine, Vol. 287, Issue 3 https://doi.org/10.1111/joim.13007	journal	November 2019

Similar Records

Structural and Functional Characterization of Aerobactin Synthetase IucA from a Hypervirulent Pathotype of Klebsiella pneumoniae

Journal Article · Thu Jun 02 00:00:00 EDT 2016 · Biochemistry · OSTI ID:1439640

Bailey, Daniel C.; Drake, Eric J.; Grant, Thomas D.; +1 more

Genomic surveillance indicates clonal replacement of hypervirulent Klebsiella pneumoniae ST881 and ST29 lineage strains in vivo

Journal Article · Mon Feb 19 00:00:00 EST 2024 · Frontiers in Microbiology · OSTI ID:1439640

Liu, Ning; Lou, Ningjie; Huang, Jiajie; +6 more

Crystal Structure of the Siderophore Binding Protein BauB Bound to an Unusual 2:1 Complex Between Acinetobactin and Ferric Iron

Journal Article · Thu Nov 08 00:00:00 EST 2018 · Biochemistry · OSTI ID:1439640

Bailey, Daniel C.; Bohac, Tabbetha J.; Shapiro, Justin A.; +3 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
siderophore
natural product biosynthesis
structural biology
enzyme structure
Klebsiella pneumonia
protein structure
X-ray crystallography
Aerobactin
Surface Entropy Reduction

Title: Structural and functional delineation of aerobactin biosynthesis in hypervirulent Klebsiella pneumoniae

Citation Formats

References (69)

Cited By (4)

Similar Records

Related Subjects