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Title: Coping with iron limitation: A metabolomic study of Synechocystis sp. PCC 6803

Abstract

Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P and higher ratios of Protein:RNA, C:N, C:P and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. Additionally, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, maymore » further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1439015
Report Number(s):
PNNL-SA-132877
Journal ID: ISSN 0137-5881; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Acta Physiologiae Plantarum
Additional Journal Information:
Journal Volume: 40; Journal Issue: 2; Journal ID: ISSN 0137-5881
Country of Publication:
United States
Language:
English
Subject:
metabolomics; metallomics; iron limitation; cyanobacteria; ecological stoichiometry

Citation Formats

Rivas-Ubach, Albert, Poret-Peterson, Amisha T., Peñuelas, Josep, Sardans, Jordi, Pérez-Trujillo, Míriam, Legido-Quigley, Cristina, Oravec, Michal, Urban, Otmar, and Elser, James J. Coping with iron limitation: A metabolomic study of Synechocystis sp. PCC 6803. United States: N. p., 2018. Web. doi:10.1007/s11738-018-2603-1.
Rivas-Ubach, Albert, Poret-Peterson, Amisha T., Peñuelas, Josep, Sardans, Jordi, Pérez-Trujillo, Míriam, Legido-Quigley, Cristina, Oravec, Michal, Urban, Otmar, & Elser, James J. Coping with iron limitation: A metabolomic study of Synechocystis sp. PCC 6803. United States. doi:10.1007/s11738-018-2603-1.
Rivas-Ubach, Albert, Poret-Peterson, Amisha T., Peñuelas, Josep, Sardans, Jordi, Pérez-Trujillo, Míriam, Legido-Quigley, Cristina, Oravec, Michal, Urban, Otmar, and Elser, James J. Tue . "Coping with iron limitation: A metabolomic study of Synechocystis sp. PCC 6803". United States. doi:10.1007/s11738-018-2603-1.
@article{osti_1439015,
title = {Coping with iron limitation: A metabolomic study of Synechocystis sp. PCC 6803},
author = {Rivas-Ubach, Albert and Poret-Peterson, Amisha T. and Peñuelas, Josep and Sardans, Jordi and Pérez-Trujillo, Míriam and Legido-Quigley, Cristina and Oravec, Michal and Urban, Otmar and Elser, James J.},
abstractNote = {Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA:DNA, C, N, and P and higher ratios of Protein:RNA, C:N, C:P and N:P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C:P and N:P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. Additionally, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.},
doi = {10.1007/s11738-018-2603-1},
journal = {Acta Physiologiae Plantarum},
issn = {0137-5881},
number = 2,
volume = 40,
place = {United States},
year = {2018},
month = {1}
}

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