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Title: Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy

Abstract

Constant mode ambient mass spectrometry imaging (MSI) of tissue sections with high lateral resolution of better than 10 µm was performed by combining shear force microscopy with nanospray desorption electrospray ionization (nano-DESI). Shear force microscopy enabled precise control of the distance between the sample and nano-DESI probe during MSI experiments and provided information on sample topography. Proof-of-concept experiments were performed using lung and brain tissue sections representing spongy and dense tissues, respectively. Topography images obtained using shear force microscopy were comparable to the results obtained using contact profilometry over the same region of the tissue section. Variations in tissue height were found to be dependent on the tissue type and were in the range of 0-5 µm for lung tissue and 0-3 µm for brain tissue sections. Ion images of phospholipids obtained in this study are in good agreement with literature data. Normalization of nano-DESI MSI images to the signal of the internal standard added to the extraction solvent allowed us to construct high-resolution ion images free of matrix effects.

Authors:
; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1439009
Report Number(s):
PNNL-SA-126302
Journal ID: ISSN 1044-0305; 49133; 49560; 48632; 453040220; 400412000
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of the American Society for Mass Spectrometry
Additional Journal Information:
Journal Volume: 29; Journal Issue: 2; Journal ID: ISSN 1044-0305
Publisher:
American Society for Mass Spectrometry
Country of Publication:
United States
Language:
English
Subject:
high-resolution imaging mass spectrometry; nanospray desorption electrospray ionization (nano-DESI); shear force microscopy; mouse lung tissue; phospholipids; metabolites; Environmental Molecular Sciences Laboratory

Citation Formats

Nguyen, Son N., Sontag, Ryan L., Carson, James P., Corley, Richard A., Ansong, Charles, and Laskin, Julia. Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy. United States: N. p., 2017. Web. doi:10.1007/s13361-017-1750-8.
Nguyen, Son N., Sontag, Ryan L., Carson, James P., Corley, Richard A., Ansong, Charles, & Laskin, Julia. Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy. United States. doi:10.1007/s13361-017-1750-8.
Nguyen, Son N., Sontag, Ryan L., Carson, James P., Corley, Richard A., Ansong, Charles, and Laskin, Julia. Fri . "Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy". United States. doi:10.1007/s13361-017-1750-8.
@article{osti_1439009,
title = {Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy},
author = {Nguyen, Son N. and Sontag, Ryan L. and Carson, James P. and Corley, Richard A. and Ansong, Charles and Laskin, Julia},
abstractNote = {Constant mode ambient mass spectrometry imaging (MSI) of tissue sections with high lateral resolution of better than 10 µm was performed by combining shear force microscopy with nanospray desorption electrospray ionization (nano-DESI). Shear force microscopy enabled precise control of the distance between the sample and nano-DESI probe during MSI experiments and provided information on sample topography. Proof-of-concept experiments were performed using lung and brain tissue sections representing spongy and dense tissues, respectively. Topography images obtained using shear force microscopy were comparable to the results obtained using contact profilometry over the same region of the tissue section. Variations in tissue height were found to be dependent on the tissue type and were in the range of 0-5 µm for lung tissue and 0-3 µm for brain tissue sections. Ion images of phospholipids obtained in this study are in good agreement with literature data. Normalization of nano-DESI MSI images to the signal of the internal standard added to the extraction solvent allowed us to construct high-resolution ion images free of matrix effects.},
doi = {10.1007/s13361-017-1750-8},
journal = {Journal of the American Society for Mass Spectrometry},
issn = {1044-0305},
number = 2,
volume = 29,
place = {United States},
year = {2017},
month = {7}
}

Works referenced in this record:

Compound and metabolite distribution measured by MALDI mass spectrometric imaging in whole-body tissue sections
journal, February 2007

  • Stoeckli, Markus; Staab, Dieter; Schweitzer, Alain
  • International Journal of Mass Spectrometry, Vol. 260, Issue 2-3
  • DOI: 10.1016/j.ijms.2006.10.007

Scanning probe electrospray ionization for ambient mass spectrometry: SPESI for ambient mass spectrometry
journal, October 2012

  • Otsuka, Yoichi; Shide, Sayuri; Naito, Junpei
  • Rapid Communications in Mass Spectrometry, Vol. 26, Issue 23
  • DOI: 10.1002/rcm.6399

Surface analysis of lipids by mass spectrometry: More than just imaging
journal, October 2013


Automation of a Surface Sampling Probe/Electrospray Mass Spectrometry System
journal, November 2005

  • Kertesz, Vilmos; Ford, Michael J.; Van Berkel, Gary J.
  • Analytical Chemistry, Vol. 77, Issue 22
  • DOI: 10.1021/ac0510742

Constant-Distance Mode Scanning Electrochemical Microscopy (SECM)—Part I: Adaptation of a Non-Optical Shear-Force-Based Positioning Mode for SECM Tips
journal, May 2003

  • Ballesteros Katemann, Bernardo; Schulte, Albert; Schuhmann, Wolfgang
  • Chemistry - A European Journal, Vol. 9, Issue 9
  • DOI: 10.1002/chem.200204267

MALDI imaging MS of phospholipids in the mouse lung
journal, April 2011

  • Berry, Karin A. Zemski; Li, Bilan; Reynolds, Susan D.
  • Journal of Lipid Research, Vol. 52, Issue 8
  • DOI: 10.1194/jlr.M015750

Mass spectrometry imaging under ambient conditions
journal, September 2012

  • Wu, Chunping; Dill, Allison L.; Eberlin, Livia S.
  • Mass Spectrometry Reviews, Vol. 32, Issue 3
  • DOI: 10.1002/mas.21360

Easy Ambient Sonic-Spray Ionization-Membrane Interface Mass Spectrometry for Direct Analysis of Solution Constituents
journal, February 2008

  • Haddad, Renato; Sparrapan, Regina; Kotiaho, Tapio
  • Analytical Chemistry, Vol. 80, Issue 3
  • DOI: 10.1021/ac701960q

Stimulated Bronchial Epithelial Cells Release Bioactive Lysophosphatidylcholine 16:0, 18:0, and 18:1
journal, January 2014

  • Zhuge, Yan; Yuan, Yang; van Breemen, Richard
  • Allergy, Asthma & Immunology Research, Vol. 6, Issue 1
  • DOI: 10.4168/aair.2014.6.1.66

Mass spectrometry imaging for drugs and metabolites
journal, November 2011


Shotgun Approach for Quantitative Imaging of Phospholipids Using Nanospray Desorption Electrospray Ionization Mass Spectrometry
journal, January 2014

  • Lanekoff, Ingela; Thomas, Mathew; Laskin, Julia
  • Analytical Chemistry, Vol. 86, Issue 3
  • DOI: 10.1021/ac403931r

Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples with Complex Topography
journal, December 2016


Ambient Mass Spectrometry
journal, March 2006


Mechanisms of Real-Time, Proximal Sample Processing during Ambient Ionization Mass Spectrometry
journal, December 2013

  • Venter, Andre R.; Douglass, Kevin A.; Shelley, Jacob T.
  • Analytical Chemistry, Vol. 86, Issue 1
  • DOI: 10.1021/ac4038569

Mass spectrometry imaging with high resolution in mass and space
journal, May 2013


Lipid mapping of the rat brain for models of disease
journal, September 2017

  • Martínez-Gardeazabal, J.; González de San Román, E.; Moreno-Rodríguez, M.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1859, Issue 9
  • DOI: 10.1016/j.bbamem.2017.02.011

Differential localization of sphingomyelin synthase isoforms in neurons regulates sphingomyelin cluster formation
journal, January 2012

  • Kidani, Yujiro; Ohshima, Ken-ichi; Sakai, Hideyuki
  • Biochemical and Biophysical Research Communications, Vol. 417, Issue 3
  • DOI: 10.1016/j.bbrc.2011.12.079

Histology by Mass Spectrometry: Label-Free Tissue Characterization Obtained from High-Accuracy Bioanalytical Imaging
journal, April 2010

  • Römpp, Andreas; Guenther, Sabine; Schober, Yvonne
  • Angewandte Chemie International Edition, Vol. 49, Issue 22
  • DOI: 10.1002/anie.200905559

Fully automated liquid extraction-based surface sampling and ionization using a chip-based robotic nanoelectrospray platform
journal, March 2010

  • Kertesz, Vilmos; Van Berkel, Gary J.
  • Journal of Mass Spectrometry, Vol. 45, Issue 3
  • DOI: 10.1002/jms.1709

Ambient desorption ionization mass spectrometry
journal, April 2008

  • Venter, Andre; Nefliu, Marcela; Graham Cooks, R.
  • TrAC Trends in Analytical Chemistry, Vol. 27, Issue 4
  • DOI: 10.1016/j.trac.2008.01.010

Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques
journal, November 2015


Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
journal, October 2004


The Single-Probe: A Miniaturized Multifunctional Device for Single Cell Mass Spectrometry Analysis
journal, September 2014

  • Pan, Ning; Rao, Wei; Kothapalli, Naga Rama
  • Analytical Chemistry, Vol. 86, Issue 19
  • DOI: 10.1021/ac5029038

Ambient Sampling/Ionization Mass Spectrometry: Applications and Current Trends
journal, June 2011

  • Harris, Glenn A.; Galhena, Asiri S.; Fernández, Facundo M.
  • Analytical Chemistry, Vol. 83, Issue 12, p. 4508-4538
  • DOI: 10.1021/ac200918u

Automated Platform for High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry
journal, September 2012

  • Lanekoff, Ingela; Heath, Brandi S.; Liyu, Andrey
  • Analytical Chemistry, Vol. 84, Issue 19
  • DOI: 10.1021/ac301909a

The role of lipids in pulmonary surfactant
journal, November 1998

  • Veldhuizen, Ruud; Nag, Kaushik; Orgeig, Sandra
  • Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol. 1408, Issue 2-3
  • DOI: 10.1016/S0925-4439(98)00061-1

What can we learn from ambient ionization techniques?
journal, November 2009

  • Chen, Huanwen; Gamez, Gerardo; Zenobi, Renato
  • Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 11
  • DOI: 10.1016/j.jasms.2009.07.025

Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology
journal, October 2005

  • Takáts, Zoltán; Wiseman, Justin M.; Cooks, R. Graham
  • Journal of Mass Spectrometry, Vol. 40, Issue 10
  • DOI: 10.1002/jms.922

Distance control in near-field optical microscopy with piezoelectrical shear-force detection suitable for imaging in liquids
journal, April 1997

  • Brunner, R.; Bietsch, A.; Hollricher, O.
  • Review of Scientific Instruments, Vol. 68, Issue 4
  • DOI: 10.1063/1.1147990

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry
journal, September 2008

  • Van Berkel, Gary J.; Pasilis, Sofie P.; Ovchinnikova, Olga
  • Journal of Mass Spectrometry, Vol. 43, Issue 9
  • DOI: 10.1002/jms.1440

Imaging Nicotine in Rat Brain Tissue by Use of Nanospray Desorption Electrospray Ionization Mass Spectrometry
journal, December 2012

  • Lanekoff, Ingela; Thomas, Mathew; Carson, James P.
  • Analytical Chemistry, Vol. 85, Issue 2
  • DOI: 10.1021/ac302308p

Matrix effects in biological mass spectrometry imaging: identification and compensation
journal, January 2014

  • Lanekoff, Ingela; Stevens, Susan L.; Stenzel-Poore, Mary P.
  • The Analyst, Vol. 139, Issue 14
  • DOI: 10.1039/c4an00504j

Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections
journal, January 2007

  • Chaurand, Pierre; Schriver, Kenneth E.; Caprioli, Richard M.
  • Journal of Mass Spectrometry, Vol. 42, Issue 4
  • DOI: 10.1002/jms.1180

A comparison of the molecular species compositions of mammalian lung surfactant phospholipids
journal, May 2001

  • Postle, Anthony D.; Heeley, Emma L.; Wilton, David C.
  • Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Vol. 129, Issue 1
  • DOI: 10.1016/S1095-6433(01)00306-3