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Title: Pyrene hydrogel for promoting direct bioelectrochemistry: ATP-independent electroenzymatic reduction of N2

Journal Article · · Chemical Science
DOI:https://doi.org/10.1039/C8SC01638K· OSTI ID:1438937
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  1. Department of Chemistry, University of Utah, Salt Lake City, USA
  2. Department of Chemistry, University of Utah, Salt Lake City, USA, Department of Chemistry
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Enzymatic bioelectrocatalysis often requires an artificial redox mediator to observe significant electron transfer rates. The use of such mediators can add a substantial overpotential and obfuscate the protein's native kinetics, which limits the voltage of a biofuel cell and alters the analytical performance of biosensors. Herein, we describe a material for facilitating direct electrochemical communication with redox proteins based on a novel pyrene-modified linear poly(ethyleneimine). This method was applied for promoting direct bioelectrocatalytic reduction of O2 by laccase and, by immobilizing the catalytic subunit of nitrogenase (MoFe protein), to demonstrate the ATP-independent direct electroenzymatic reduction of N2 to NH3.

Research Organization:
Fulcrum BioScience, Inc., Salt Lake City, UT (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office
Grant/Contract Number:
SC0017845
OSTI ID:
1438937
Alternate ID(s):
OSTI ID: 1466824
Journal Information:
Chemical Science, Journal Name: Chemical Science Vol. 9 Journal Issue: 23; ISSN 2041-6520
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 42 works
Citation information provided by
Web of Science

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Cited By (8)

Electrochemical Characterization of Isolated Nitrogenase Cofactors from Azotobacter vinelandii journal October 2019
Pyrene‐Based Noncovalent Immobilization of Nitrogenase on Carbon Surfaces journal January 2020
Strategies for Bioelectrochemical CO 2 Reduction journal September 2019
Templating Porphyrin Anisotropy via Magnetically Aligned Carbon Nanotubes journal February 2019
Intracellular dark-field imaging of ATP and photothermal therapy using a colorimetric assay based on gold nanoparticle aggregation via tetrazine/trans-cyclooctene cycloaddition journal July 2019
Interfacing nature’s catalytic machinery with synthetic materials for semi-artificial photosynthesis journal October 2018
The progress and outlook of bioelectrocatalysis for the production of chemicals, fuels and materials journal January 2020
Direct bioelectrocatalysis by redox enzymes immobilized in electrostatically condensed oppositely charged polyelectrolyte electrode coatings journal January 2020

Figures / Tables (6)

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Table 1(p. 5)table Table 1

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
enzyme
ammonia
hydrogel
Haber-Bosch