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Title: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface

Authors:
ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1438840
Resource Type:
Journal Article
Journal Name:
Science
Additional Journal Information:
Journal Volume: 360; Journal Issue: 6388; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Jenni, Simon, and Harrison, Stephen C. Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. United States: N. p., 2018. Web. doi:10.1126/science.aar6436.
Jenni, Simon, & Harrison, Stephen C. Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. United States. doi:10.1126/science.aar6436.
Jenni, Simon, and Harrison, Stephen C. Thu . "Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface". United States. doi:10.1126/science.aar6436.
@article{osti_1438840,
title = {Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface},
author = {Jenni, Simon and Harrison, Stephen C.},
abstractNote = {},
doi = {10.1126/science.aar6436},
journal = {Science},
issn = {0036-8075},
number = 6388,
volume = 360,
place = {United States},
year = {2018},
month = {5}
}

Works referenced in this record:

Automated tilt series alignment and tomographic reconstruction in IMOD
journal, February 2017


Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B
journal, May 2010

  • Tien, Jerry F.; Umbreit, Neil T.; Gestaut, Daniel R.
  • The Journal of Cell Biology, Vol. 189, Issue 4
  • DOI: 10.1083/jcb.200910142

Phospho-Regulation of Kinetochore-Microtubule Attachments by the Aurora Kinase Ipl1p
journal, October 2002


Fast pseudolikelihood maximization for direct-coupling analysis of protein structure from many homologous amino-acid sequences
journal, November 2014


The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End
journal, February 2011


De novo protein structure determination from near-atomic-resolution cryo-EM maps
journal, February 2015

  • Wang, Ray Yu-Ruei; Kudryashev, Mikhail; Li, Xueming
  • Nature Methods, Vol. 12, Issue 4
  • DOI: 10.1038/nmeth.3287

The Ndc80 complex bridges two Dam1 complex rings
journal, February 2017


Biopython: freely available Python tools for computational molecular biology and bioinformatics
journal, March 2009


Molecular organization of the Ndc80 complex, an essential kinetochore component
journal, April 2005

  • Wei, R. R.; Sorger, P. K.; Harrison, S. C.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 15
  • DOI: 10.1073/pnas.0501168102

Global analysis of protein expression in yeast
journal, October 2003

  • Ghaemmaghami, Sina; Huh, Won-Ki; Bower, Kiowa
  • Nature, Vol. 425, Issue 6959
  • DOI: 10.1038/nature02046

Improved contact prediction in proteins: Using pseudolikelihoods to infer Potts models
journal, January 2013


Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface
journal, May 2012


Molecular Structures of Yeast Kinetochore Subcomplexes and Their Roles in Chromosome Segregation
journal, January 2017

  • Jenni, Simon; Dimitrova, Yoana N.; Valverde, Roberto
  • Cold Spring Harbor Symposia on Quantitative Biology, Vol. 82
  • DOI: 10.1101/sqb.2017.82.033738

Microtubule depolymerization can drive poleward chromosome motion in fission yeast
journal, October 2006


Conserved and divergent features of kinetochores and spindle microtubule ends from five species
journal, February 2013

  • McIntosh, J. Richard; O’Toole, Eileen; Zhudenkov, Kirill
  • The Journal of Cell Biology, Vol. 200, Issue 4
  • DOI: 10.1083/jcb.201209154

XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009

  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318

Architecture and Flexibility of the Yeast Ndc80 Kinetochore Complex
journal, November 2008

  • Wang, Hong-Wei; Long, Sydney; Ciferri, Claudio
  • Journal of Molecular Biology, Vol. 383, Issue 4
  • DOI: 10.1016/j.jmb.2008.08.077

SPARX, a new environment for Cryo-EM image processing
journal, January 2007

  • Hohn, Michael; Tang, Grant; Goodyear, Grant
  • Journal of Structural Biology, Vol. 157, Issue 1
  • DOI: 10.1016/j.jsb.2006.07.003

Assembling the Protein Architecture of the Budding Yeast Kinetochore-Microtubule Attachment using FRET
journal, July 2014

  • Aravamudhan, Pavithra; Felzer-Kim, Isabella; Gurunathan, Kaushik
  • Current Biology, Vol. 24, Issue 13
  • DOI: 10.1016/j.cub.2014.05.014

Gctf: Real-time CTF determination and correction
journal, January 2016


SPRING – An image processing package for single-particle based helical reconstruction from electron cryomicrographs
journal, January 2014

  • Desfosses, Ambroise; Ciuffa, Rodolfo; Gutsche, Irina
  • Journal of Structural Biology, Vol. 185, Issue 1
  • DOI: 10.1016/j.jsb.2013.11.003

Diffraction-geometry refinement in the DIALS framework
journal, March 2016

  • Waterman, David G.; Winter, Graeme; Gildea, Richard J.
  • Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 4
  • DOI: 10.1107/S2059798316002187

SCaFoS: a tool for Selection, Concatenation and Fusion of Sequences for phylogenomics
journal, January 2007

  • Roure, Béatrice; Rodriguez-Ezpeleta, Naiara; Philippe, Hervé
  • BMC Evolutionary Biology, Vol. 7, Issue Suppl 1
  • DOI: 10.1186/1471-2148-7-S1-S2

Optimal Determination of Particle Orientation, Absolute Hand, and Contrast Loss in Single-particle Electron Cryomicroscopy
journal, October 2003


Three-Dimensional Structures of Membrane Proteins from Genomic Sequencing
journal, June 2012


Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation
journal, March 2008

  • Gestaut, Daniel R.; Graczyk, Beth; Cooper, Jeremy
  • Nature Cell Biology, Vol. 10, Issue 4
  • DOI: 10.1038/ncb1702

MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform
journal, July 2002


Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore
journal, June 2005

  • Ciferri, Claudio; De Luca, Jennifer; Monzani, Silvia
  • Journal of Biological Chemistry, Vol. 280, Issue 32
  • DOI: 10.1074/jbc.M504070200

Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle
journal, June 1995


MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073

The Dam1 complex confers microtubule plus end–tracking activity to the Ndc80 kinetochore complex
journal, May 2010

  • Lampert, Fabienne; Hornung, Peter; Westermann, Stefan
  • The Journal of Cell Biology, Vol. 189, Issue 4
  • DOI: 10.1083/jcb.200912021

Molecular basis of outer kinetochore assembly on CENP-T
journal, December 2016

  • Huis in 't Veld, Pim J.; Jeganathan, Sadasivam; Petrovic, Arsen
  • eLife, Vol. 5
  • DOI: 10.7554/eLife.21007

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Molecular requirements for the formation of a kinetochore–microtubule interface by Dam1 and Ndc80 complexes
journal, December 2012

  • Lampert, Fabienne; Mieck, Christine; Alushin, Gregory M.
  • The Journal of Cell Biology, Vol. 200, Issue 1
  • DOI: 10.1083/jcb.201210091

Centromere identity is specified by a single centromeric nucleosome in budding yeast
journal, September 2007

  • Furuyama, S.; Biggins, S.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 37
  • DOI: 10.1073/pnas.0706985104

Molecular mechanisms of microtubule-dependent kinetochore transport toward spindle poles
journal, July 2007

  • Tanaka, Kozo; Kitamura, Etsushi; Kitamura, Yoko
  • The Journal of Cell Biology, Vol. 178, Issue 2
  • DOI: 10.1083/jcb.200702141

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004

  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340

ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules
journal, May 2016

  • Ashkenazy, Haim; Abadi, Shiran; Martz, Eric
  • Nucleic Acids Research, Vol. 44, Issue W1
  • DOI: 10.1093/nar/gkw408

T-coffee: a novel method for fast and accurate multiple sequence alignment 1 1Edited by J. Thornton
journal, September 2000

  • Notredame, Cédric; Higgins, Desmond G.; Heringa, Jaap
  • Journal of Molecular Biology, Vol. 302, Issue 1
  • DOI: 10.1006/jmbi.2000.4042

MycoCosm portal: gearing up for 1000 fungal genomes
journal, December 2013

  • Grigoriev, Igor V.; Nikitin, Roman; Haridas, Sajeet
  • Nucleic Acids Research, Vol. 42, Issue D1
  • DOI: 10.1093/nar/gkt1183

SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs
journal, October 2008

  • Shaikh, Tanvir R.; Gao, Haixiao; Baxter, William T.
  • Nature Protocols, Vol. 3, Issue 12
  • DOI: 10.1038/nprot.2008.156

Protein secondary structure prediction based on position-specific scoring matrices 1 1Edited by G. Von Heijne
journal, September 1999


Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms
journal, July 2007

  • Wang, Hong-Wei; Ramey, Vincent H.; Westermann, Stefan
  • Nature Structural & Molecular Biology, Vol. 14, Issue 8
  • DOI: 10.1038/nsmb1274

Direct-coupling analysis of residue coevolution captures native contacts across many protein families
journal, November 2011

  • Morcos, F.; Pagnani, A.; Lunt, B.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 49
  • DOI: 10.1073/pnas.1111471108

Structural Analysis of Lac Repressor Bound to Allosteric Effectors
journal, July 2007

  • Daber, Robert; Stayrook, Steven; Rosenberg, Allison
  • Journal of Molecular Biology, Vol. 370, Issue 4
  • DOI: 10.1016/j.jmb.2007.04.028

A robust algorithm for the reconstruction of helical filaments using single-particle methods
journal, December 2000


High-Resolution Comparative Modeling with RosettaCM
journal, October 2013


DelPhi: a comprehensive suite for DelPhi software and associated resources
journal, January 2012


GenBank
journal, November 2012

  • Benson, Dennis A.; Cavanaugh, Mark; Clark, Karen
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1195

Mechanistic Origin of Microtubule Dynamic Instability and Its Modulation by EB Proteins
journal, August 2015


Conserved Tetramer Junction in the Kinetochore Ndc80 Complex
journal, November 2016


The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards
journal, July 2013

  • Briegel, Ariane; Pilhofer, Martin; Mastronarde, David N.
  • Journal of Structural Biology, Vol. 183, Issue 1
  • DOI: 10.1016/j.jsb.2013.04.008

CCAN Assembly Configures Composite Binding Interfaces to Promote Cross-Linking of Ndc80 Complexes at the Kinetochore
journal, September 2016

  • Pekgöz Altunkaya, Gülsah; Malvezzi, Francesca; Demianova, Zuzana
  • Current Biology, Vol. 26, Issue 17
  • DOI: 10.1016/j.cub.2016.07.005

High resolution single particle refinement in EMAN2.1
journal, May 2016


The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling
journal, November 2015

  • Zelter, Alex; Bonomi, Massimiliano; Kim, Jae ook
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms9673

CTFFIND4: Fast and accurate defocus estimation from electron micrographs
journal, November 2015


Automatic estimation and correction of anisotropic magnification distortion in electron microscopes
journal, November 2015


Improved methods for building protein models in electron density maps and the location of errors in these models
journal, March 1991

  • Jones, T. A.; Zou, J. Y.; Cowan, S. W.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119
  • DOI: 10.1107/S0108767390010224

Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly
journal, November 2016


PredictProtein—an open resource for online prediction of protein structural and functional features
journal, May 2014

  • Yachdav, Guy; Kloppmann, Edda; Kajan, Laszlo
  • Nucleic Acids Research, Vol. 42, Issue W1
  • DOI: 10.1093/nar/gku366

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
journal, February 2017

  • Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul
  • Nature Methods, Vol. 14, Issue 4
  • DOI: 10.1038/nmeth.4193

The protelomerase of temperate Escherichia coli phage N15 has cleaving-joining activity
journal, July 2000

  • Deneke, J.; Ziegelin, G.; Lurz, R.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 14
  • DOI: 10.1073/pnas.97.14.7721

Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
journal, November 2011

  • Ramey, Vincent H.; Wong, Amanda; Fang, Jie
  • Molecular Biology of the Cell, Vol. 22, Issue 22
  • DOI: 10.1091/mbc.E11-07-0659

The Budding Yeast Point Centromere Associates with Two Cse4 Molecules during Mitosis
journal, May 2013

  • Aravamudhan, Pavithra; Felzer-Kim, Isabella; Joglekar, Ajit P.
  • Current Biology, Vol. 23, Issue 9
  • DOI: 10.1016/j.cub.2013.03.042

Quantifying the local resolution of cryo-EM density maps
journal, November 2013

  • Kucukelbir, Alp; Sigworth, Fred J.; Tagare, Hemant D.
  • Nature Methods, Vol. 11, Issue 1
  • DOI: 10.1038/nmeth.2727

Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex
journal, July 2007

  • Miranda, Jj L.; King, David S.; Harrison, Stephen C.
  • Molecular Biology of the Cell, Vol. 18, Issue 7
  • DOI: 10.1091/mbc.E07-02-0135

AUGUSTUS: a web server for gene finding in eukaryotes
journal, July 2004

  • Stanke, M.; Steinkamp, R.; Waack, S.
  • Nucleic Acids Research, Vol. 32, Issue Web Server
  • DOI: 10.1093/nar/gkh379

Chromatin Signaling to Kinetochores: Transregulation of Dam1 Methylation by Histone H2B Ubiquitination
journal, September 2011


The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule
journal, February 2011

  • Ramey, Vincent H.; Wang, Hong-Wei; Nakajima, Yuko
  • Molecular Biology of the Cell, Vol. 22, Issue 4
  • DOI: 10.1091/mbc.E10-10-0841

Effect of DNA denaturants on the lac repressor-operator interaction
journal, September 1978

  • Pfahl, Magnus
  • Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Vol. 520, Issue 2
  • DOI: 10.1016/0005-2787(78)90227-7

Kinetochores’ gripping feat: conformational wave or biased diffusion?
journal, January 2011

  • Asbury, Charles L.; Tien, Jerry F.; Davis, Trisha N.
  • Trends in Cell Biology, Vol. 21, Issue 1
  • DOI: 10.1016/j.tcb.2010.09.003

Dephosphorylation of the Ndc80 Tail Stabilizes Kinetochore-Microtubule Attachments via the Ska Complex
journal, May 2017


Molecular architecture of the Dam1 complex–microtubule interaction
journal, March 2016

  • Legal, Thibault; Zou, Juan; Sochaj, Alicja
  • Open Biology, Vol. 6, Issue 3
  • DOI: 10.1098/rsob.150237

trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses
journal, June 2009


Deciphering key features in protein structures with the new ENDscript server
journal, April 2014

  • Robert, Xavier; Gouet, Patrice
  • Nucleic Acids Research, Vol. 42, Issue W1
  • DOI: 10.1093/nar/gku316

Analysis of kinesin motor function at budding yeast kinetochores
journal, March 2006

  • Tytell, Jessica D.; Sorger, Peter K.
  • The Journal of Cell Biology, Vol. 172, Issue 6
  • DOI: 10.1083/jcb.200509101

RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012


The yeast DASH complex forms closed rings on microtubules
journal, January 2005

  • Miranda, Jj L.; Wulf, Peter De; Sorger, Peter K.
  • Nature Structural & Molecular Biology, Vol. 12, Issue 2
  • DOI: 10.1038/nsmb896

BLAST+: architecture and applications
journal, January 2009

  • Camacho, Christiam; Coulouris, George; Avagyan, Vahram
  • BMC Bioinformatics, Vol. 10, Issue 1
  • DOI: 10.1186/1471-2105-10-421

The Composition, Functions, and Regulation of the Budding Yeast Kinetochore
journal, August 2013


Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores
journal, May 2017


Polewards chromosome movement driven by microtubule depolymerization in vitro
journal, February 1988

  • Koshland, Douglas E.; Mitchison, T. J.; Kirschner, Marc W.
  • Nature, Vol. 331, Issue 6156
  • DOI: 10.1038/331499a0

Unique Phylogenetic Distributions of the Ska and Dam1 Complexes Support Functional Analogy and Suggest Multiple Parallel Displacements of Ska by Dam1
journal, May 2017

  • van Hooff, Jolien J. E.; Snel, Berend; Kops, Geert J. P. L.
  • Genome Biology and Evolution, Vol. 9, Issue 5
  • DOI: 10.1093/gbe/evx088

Mapping of I gene mutations which lead to repressors with increased affinity for lac operator
journal, March 1981


Coiled-coil networking shapes cell molecular machinery
journal, October 2012

  • Wang, Yongqiang; Zhang, Xinlei; Zhang, Hong
  • Molecular Biology of the Cell, Vol. 23, Issue 19
  • DOI: 10.1091/mbc.E12-05-0396

EMBOSS: The European Molecular Biology Open Software Suite
journal, June 2000


Real-space processing of helical filaments in SPARX
journal, February 2012

  • Behrmann, Elmar; Tao, Guozhi; Stokes, David L.
  • Journal of Structural Biology, Vol. 177, Issue 2
  • DOI: 10.1016/j.jsb.2011.12.020

Tomato bushy stunt virus at 2.9 Å resolution
journal, November 1978

  • Harrison, S. C.; Olson, A. J.; Schutt, C. E.
  • Nature, Vol. 276, Issue 5686
  • DOI: 10.1038/276368a0

Structural Analysis of Multiprotein Complexes by Cross-linking, Mass Spectrometry, and Database Searching
journal, October 2007

  • Maiolica, Alessio; Cittaro, Davide; Borsotti, Dario
  • Molecular & Cellular Proteomics, Vol. 6, Issue 12
  • DOI: 10.1074/mcp.M700274-MCP200

Anaphase A: Disassembling Microtubules Move Chromosomes toward Spindle Poles
journal, February 2017


Formation of a Dynamic Kinetochore- Microtubule Interface through Assembly of the Dam1 Ring Complex
journal, January 2005


The Ndc80 kinetochore complex forms oligomeric arrays along microtubules
journal, October 2010

  • Alushin, Gregory M.; Ramey, Vincent H.; Pasqualato, Sebastiano
  • Nature, Vol. 467, Issue 7317
  • DOI: 10.1038/nature09423