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Title: Plant and soil effects on bacterial communities associated with Miscanthus  ×  giganteus rhizosphere and rhizomes

Abstract

Here, bacterial assemblages, especially diazotroph assemblages residing in the rhizomes and the rhizosphere soil of Miscanthus × giganteus, contribute to plant growth and nitrogen use efficiency. However, the composition of these microbial communities has not been adequately explored nor have the potential ecological drivers for these communities been sufficiently studied. This knowledge is needed for understanding and potentially improving M. × giganteus – microbe interactions, and further enhancing sustainability of M. × giganteus production. In this study, cultivated M. × giganteus from four sites in Illinois, Kentucky, Nebraska, and New Jersey were collected to examine the relative influences of soil conditions and plant compartments on assembly of the M. × giganteus-associated microbiome. Automated ribosomal intergenic spacer (ARISA) and terminal restriction fragment length polymorphism (T-RFLP) targeting the nifH gene were applied to examine the total bacterial communities and diazotroph assemblages that reside in the rhizomes and the rhizosphere. Distinct microbial assemblages were detected in the endophytic and rhizosphere compartments. Site soil conditions had strong correlation with both total bacterial and diazotroph assemblages, but in different ways. Nitrogen treatments showed no significant effect on the composition of diazotroph assemblages in most sites. Endophytic compartments of different M. × giganteus plants tended tomore » harbor similar microbial communities across all sites, whereas the rhizosphere soil of different plant tended to harbor diverse microbial assemblages that were distinct among sites. These observations offer insight into better understanding of the associative interactions between M. × giganteus and diazotrophs, and how this relationship is influenced by agronomic and edaphic factors.« less

Authors:
ORCiD logo [1];  [2];  [1]
  1. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Natural Resources and Environmental Sciences
  2. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Crop Sciences
Publication Date:
Research Org.:
South Dakota State Univ., Brookings, SD (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B); Univ. of Illinois at Urbana-Champaign, IL (United States). Energy Farm; U.S. Department of Agriculture (USDA). Cooperative State Research, Education, and Extension Service (CSREES)
OSTI Identifier:
1438470
Alternate Identifier(s):
OSTI ID: 1361197; OSTI ID: 1400692
Grant/Contract Number:
FC36-05GO85041; ILLU 875‐374
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Global Change Biology. Bioenergy
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1757-1693
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; bacterial community composition; bioenergy feedstock; diazotroph; endophytic compartment; Miscanthus x giganteus; rhizosphere; Miscanthus 9 giganteus

Citation Formats

Li, Dongfang, Voigt, Thomas B., and Kent, Angela D. Plant and soil effects on bacterial communities associated with Miscanthus  ×  giganteus rhizosphere and rhizomes. United States: N. p., 2015. Web. doi:10.1111/gcbb.12252.
Li, Dongfang, Voigt, Thomas B., & Kent, Angela D. Plant and soil effects on bacterial communities associated with Miscanthus  ×  giganteus rhizosphere and rhizomes. United States. doi:10.1111/gcbb.12252.
Li, Dongfang, Voigt, Thomas B., and Kent, Angela D. Thu . "Plant and soil effects on bacterial communities associated with Miscanthus  ×  giganteus rhizosphere and rhizomes". United States. doi:10.1111/gcbb.12252. https://www.osti.gov/servlets/purl/1438470.
@article{osti_1438470,
title = {Plant and soil effects on bacterial communities associated with Miscanthus  ×  giganteus rhizosphere and rhizomes},
author = {Li, Dongfang and Voigt, Thomas B. and Kent, Angela D.},
abstractNote = {Here, bacterial assemblages, especially diazotroph assemblages residing in the rhizomes and the rhizosphere soil of Miscanthus × giganteus, contribute to plant growth and nitrogen use efficiency. However, the composition of these microbial communities has not been adequately explored nor have the potential ecological drivers for these communities been sufficiently studied. This knowledge is needed for understanding and potentially improving M. × giganteus – microbe interactions, and further enhancing sustainability of M. × giganteus production. In this study, cultivated M. × giganteus from four sites in Illinois, Kentucky, Nebraska, and New Jersey were collected to examine the relative influences of soil conditions and plant compartments on assembly of the M. × giganteus-associated microbiome. Automated ribosomal intergenic spacer (ARISA) and terminal restriction fragment length polymorphism (T-RFLP) targeting the nifH gene were applied to examine the total bacterial communities and diazotroph assemblages that reside in the rhizomes and the rhizosphere. Distinct microbial assemblages were detected in the endophytic and rhizosphere compartments. Site soil conditions had strong correlation with both total bacterial and diazotroph assemblages, but in different ways. Nitrogen treatments showed no significant effect on the composition of diazotroph assemblages in most sites. Endophytic compartments of different M. × giganteus plants tended to harbor similar microbial communities across all sites, whereas the rhizosphere soil of different plant tended to harbor diverse microbial assemblages that were distinct among sites. These observations offer insight into better understanding of the associative interactions between M. × giganteus and diazotrophs, and how this relationship is influenced by agronomic and edaphic factors.},
doi = {10.1111/gcbb.12252},
journal = {Global Change Biology. Bioenergy},
number = 1,
volume = 8,
place = {United States},
year = {Thu Apr 30 00:00:00 EDT 2015},
month = {Thu Apr 30 00:00:00 EDT 2015}
}

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