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Title: DNA-cell conjugates

Abstract

The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.

Inventors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1438396
Patent Number(s):
9,969,970
Application Number:
15/057,604
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (Oakland, CA) LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Mar 01
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hsiao, Shih-Chia, Francis, Matthew B., Bertozzi, Carolyn, Mathies, Richard, Chandra, Ravi, Douglas, Erik, Twite, Amy, Toriello, Nicholas, and Onoe, Hiroaki. DNA-cell conjugates. United States: N. p., 2018. Web.
Hsiao, Shih-Chia, Francis, Matthew B., Bertozzi, Carolyn, Mathies, Richard, Chandra, Ravi, Douglas, Erik, Twite, Amy, Toriello, Nicholas, & Onoe, Hiroaki. DNA-cell conjugates. United States.
Hsiao, Shih-Chia, Francis, Matthew B., Bertozzi, Carolyn, Mathies, Richard, Chandra, Ravi, Douglas, Erik, Twite, Amy, Toriello, Nicholas, and Onoe, Hiroaki. Tue . "DNA-cell conjugates". United States. https://www.osti.gov/servlets/purl/1438396.
@article{osti_1438396,
title = {DNA-cell conjugates},
author = {Hsiao, Shih-Chia and Francis, Matthew B. and Bertozzi, Carolyn and Mathies, Richard and Chandra, Ravi and Douglas, Erik and Twite, Amy and Toriello, Nicholas and Onoe, Hiroaki},
abstractNote = {The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {5}
}

Patent:

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Works referenced in this record:

Direct Cell Surface Modification with DNA for the Capture of Primary Cells and the Investigation of Myotube Formation on Defined Patterns
journal, June 2009

  • Hsiao, Sonny C.; Shum, Betty J.; Onoe, Hiroaki
  • Langmuir, Vol. 25, Issue 12, p. 6985-6991
  • DOI: 10.1021/la900150n

Recognition Imaging with a DNA Aptamer
journal, June 2006


Behavior of synthetic polymers immobilized on a cell membrane
journal, April 2008


Integrated microfluidic bioprocessor for single-cell gene expression analysis
journal, December 2008

  • Toriello, N. M.; Douglas, E. S.; Thaitrong, N.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 51, p. 20173-20178
  • DOI: 10.1073/pnas.0806355106

DNA-Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells
journal, October 2008

  • Hsiao, Sonny?C.; Crow, Ailey?K.; Lam, Wilbur?A.
  • Angewandte Chemie International Edition, Vol. 47, Issue 44, p. 8473-8477
  • DOI: 10.1002/anie.200802525

Viral Capsid DNA Aptamer Conjugates as Multivalent Cell-Targeting Vehicles
journal, August 2009

  • Tong, Gary J.; Hsiao, Sonny C.; Carrico, Zachary M.
  • Journal of the American Chemical Society, Vol. 131, Issue 31, p. 11174-11178
  • DOI: 10.1021/ja903857f

A Universal Method for the Preparation of Covalent Protein�DNA Conjugates for Use in Creating Protein Nanostructures
journal, November 2007

  • Duckworth, Benjamin?P.; Chen, Yan; Wollack, James?W.
  • Angewandte Chemie International Edition, Vol. 46, Issue 46, p. 8819-8822
  • DOI: 10.1002/anie.200701942

Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address
journal, May 2006

  • Jongsma, Maarten A.; Litjens, Ralph H. G. M.
  • PROTEOMICS, Vol. 6, Issue 9, p. 2650-2655
  • DOI: 10.1002/pmic.200500654

Highly sensitive detectiong of DNA using enzyme-liked DNA-probe. I Colorimatric and fluorometric detection
journal, January 1989

  • Murakami, Akira; Tada, Jun; Yamagate, Koichi
  • Nucleic Acids Research, Vol. 17, Issue 14, p. 5587-5595
  • DOI: 10.1093/nar/17.14.5587

An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides
journal, January 2007

  • Tominaga, Jo; Kemori, Yoshinori; Tanaka, Yusuke
  • Chemical Communications, Vol. 0, Issue 4, p. 401-403
  • DOI: 10.1039/B613793H

Programmable Cell Adhesion Encoded by DNA Hybridization
journal, January 2006

  • Chandra, Ravi A.; Douglas, Erik S.; Mathies, Richard A.
  • Angewandte Chemie International Edition, Vol. 45, Issue 6, p. 896-901
  • DOI: 10.1002/anie.200502421

DNA modification of live cell surface
journal, January 2009

  • Borisenko, Grigory G.; Zaitseva, Marina A.; Chuvilin, Andrey N.
  • Nucleic Acids Research, Vol. 37, Issue 4, p. e28-e28
  • DOI: 10.1093/nar/gkn1034

DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array
journal, January 2009

  • Douglas, Erik S.; Hsiao, Sonny C.; Onoe, Hiroaki
  • Lab on a Chip, Vol. 9, Issue 14, p. 2010-2015
  • DOI: 10.1039/B821690H