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Title: Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification

Abstract

Lignin is an abundant aromatic plant cell wall polymer consisting of phenylpropanoid units in which the aromatic rings display various degrees of methoxylation. Tricin [5,7-dihydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)-4H-chromen-4-one], a flavone, was recently established as a true monomer in grass lignins. To elucidate the incorporation pathways of tricin into grass lignin, the metabolites of maize (Zea mays) were extracted from lignifying tissues and profiled using the recently developed ‘candidate substrate product pair’ algorithm applied to ultra-high-performance liquid chromatography and Fourier transform-ion cyclotron resonance-mass spectrometry. Twelve tricin-containing products (each with up to eight isomers), including those derived from the various monolignol acetate and p-coumarate conjugates, were observed and authenticated by comparisons with a set of synthetic tricin-oligolignol dimeric and trimeric compounds. The identification of such compounds helps establish that tricin is an important monomer in the lignification of monocots, acting as a nucleation site for starting lignin chains. The array of tricincontaining products provides further evidence for the combinatorial coupling model of general lignification and supports evolving paradigms for the unique nature of lignification in monocots.

Authors:
 [1];  [2];  [3];  [4];  [4];  [2];  [5];  [2];  [1]
  1. Wisconsin Energy Inst., Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
  2. Viaams Inst. voor Biotechnologie, Ghent (Belgium); Ghent Univ. (Belgium)
  3. Wisconsin Energy Inst., Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States); South China Univ. of Technology (SCUT), Guangzhou (China)
  4. Spanish National Research Council (CSIC), Seville (Spain)
  5. Univ. of Florida, Gainesville, FL (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1438204
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 171; Journal Issue: 2; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lan, Wu, Morreel, Kris, Lu, Fachuang, Rencoret, Jorge, del Rio, Jose Carlos, Voorend, Wannes, Vermerris, Wilfred, Boerjan, Wout A., and Ralph, John. Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification. United States: N. p., 2016. Web. doi:10.1104/pp.16.02012.
Lan, Wu, Morreel, Kris, Lu, Fachuang, Rencoret, Jorge, del Rio, Jose Carlos, Voorend, Wannes, Vermerris, Wilfred, Boerjan, Wout A., & Ralph, John. Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification. United States. doi:10.1104/pp.16.02012.
Lan, Wu, Morreel, Kris, Lu, Fachuang, Rencoret, Jorge, del Rio, Jose Carlos, Voorend, Wannes, Vermerris, Wilfred, Boerjan, Wout A., and Ralph, John. Wed . "Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification". United States. doi:10.1104/pp.16.02012. https://www.osti.gov/servlets/purl/1438204.
@article{osti_1438204,
title = {Maize Tricin-Oligolignol Metabolites and their Implications for Monocot Lignification},
author = {Lan, Wu and Morreel, Kris and Lu, Fachuang and Rencoret, Jorge and del Rio, Jose Carlos and Voorend, Wannes and Vermerris, Wilfred and Boerjan, Wout A. and Ralph, John},
abstractNote = {Lignin is an abundant aromatic plant cell wall polymer consisting of phenylpropanoid units in which the aromatic rings display various degrees of methoxylation. Tricin [5,7-dihydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)-4H-chromen-4-one], a flavone, was recently established as a true monomer in grass lignins. To elucidate the incorporation pathways of tricin into grass lignin, the metabolites of maize (Zea mays) were extracted from lignifying tissues and profiled using the recently developed ‘candidate substrate product pair’ algorithm applied to ultra-high-performance liquid chromatography and Fourier transform-ion cyclotron resonance-mass spectrometry. Twelve tricin-containing products (each with up to eight isomers), including those derived from the various monolignol acetate and p-coumarate conjugates, were observed and authenticated by comparisons with a set of synthetic tricin-oligolignol dimeric and trimeric compounds. The identification of such compounds helps establish that tricin is an important monomer in the lignification of monocots, acting as a nucleation site for starting lignin chains. The array of tricincontaining products provides further evidence for the combinatorial coupling model of general lignification and supports evolving paradigms for the unique nature of lignification in monocots.},
doi = {10.1104/pp.16.02012},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = 2,
volume = 171,
place = {United States},
year = {2016},
month = {6}
}

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Works referencing / citing this record:

The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9

The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9