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Title: The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin

Abstract

We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.

Authors:
 [1];  [1];  [2];  [2];  [2];  [3];  [4];  [2];  [1]
  1. Leiden Univ. (Netherlands)
  2. Univ. of Utrecht (Netherlands)
  3. Wageningen Univ. (Netherlands)
  4. Leiden Univ. (Netherlands); Univ. of Utrecht (Netherlands)
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1437993
Grant/Contract Number:  
FG02-93ER20097
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
FEBS Letters
Additional Journal Information:
Journal Volume: 590; Journal Issue: 12; Journal ID: ISSN 0014-5793
Publisher:
Federation of European Biochemical Societies
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES

Citation Formats

Alazi, Ebru, Niu, Jing, Kowalczyk, Joanna E., Peng, Mao, Aguilar Pontes, Maria Victoria, van Kan, Jan A. L., Visser, Jaap, de Vries, Ronald P., and Ram, Arthur F. J. The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin. United States: N. p., 2016. Web. doi:10.1002/1873-3468.12211.
Alazi, Ebru, Niu, Jing, Kowalczyk, Joanna E., Peng, Mao, Aguilar Pontes, Maria Victoria, van Kan, Jan A. L., Visser, Jaap, de Vries, Ronald P., & Ram, Arthur F. J. The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin. United States. doi:10.1002/1873-3468.12211.
Alazi, Ebru, Niu, Jing, Kowalczyk, Joanna E., Peng, Mao, Aguilar Pontes, Maria Victoria, van Kan, Jan A. L., Visser, Jaap, de Vries, Ronald P., and Ram, Arthur F. J. Fri . "The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin". United States. doi:10.1002/1873-3468.12211. https://www.osti.gov/servlets/purl/1437993.
@article{osti_1437993,
title = {The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin},
author = {Alazi, Ebru and Niu, Jing and Kowalczyk, Joanna E. and Peng, Mao and Aguilar Pontes, Maria Victoria and van Kan, Jan A. L. and Visser, Jaap and de Vries, Ronald P. and Ram, Arthur F. J.},
abstractNote = {We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.},
doi = {10.1002/1873-3468.12211},
journal = {FEBS Letters},
number = 12,
volume = 590,
place = {United States},
year = {Fri May 13 00:00:00 EDT 2016},
month = {Fri May 13 00:00:00 EDT 2016}
}

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Cited by: 13 works
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Works referenced in this record:

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2