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Title: Dual-Readout Immunochromatographic Assay by Utilizing MnO 2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe

Abstract

Manganese dioxide nanoflowers (MnO2 NFs) were synthesized and utilized as a dual readout probe to develop a novel immunochromatographic test strip (ITS) for detecting pesticide residues using chlorpyrifos as the model analyte. MnO2 NFs-labeled antibody for chlorpyrifos was employed as the signal tracer for conducting the ITS. After 10-min competitive immunoreaction, the tracer antibody was captured by the immobilized immunogen on test line in the test strip, resulting in the accumulation of MnO2 NFs. The accumulation of MnO2 NFs led to the appearance of brown color on the test line, which could be easily observed by the naked eye as a qualitative readout. Moreover, MnO2 NFs showed a remarkably enhancing effect on the luminol-H2O2 chemiluminescent (CL) system. Unlike peroxidase-like nanomaterials, the enhancing mechanism of MnO2 NFs was based on its oxidant activity to decompose H2O2 for forming reactive oxygen species. After initiating the CL system in the test zone, strong CL signal was collected as a quantitative readout to sensitively detect chlorpyrifos. Under optimal conditions, the linear range of chlorpyrifos was 0.1–50 ng/mL with a low detection limit of 0.033 ng/mL (S/N = 3). The reliability of the dual-readout ITS was successfully demonstrated by the application on traditional Chinese medicinemore » and environmental water samples. Due to the simultaneous rapid-qualitative and sensitive-quantitative detection, the dual-readout protocol provides a promising strategy for rapid screening and field assay on various areas such as environmental monitoring, food safety and point-of-care testing.« less

Authors:
 [1];  [2];  [3]; ORCiD logo [2];  [2]; ORCiD logo [2]; ORCiD logo [4]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [2]
  1. School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States; Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
  2. School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, United States
  3. Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
  4. Health Impacts and Exposure Science, Pacific Northwest National Laboratory, Richland, Washington 99352, United States
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1437545
Report Number(s):
PNNL-SA-129235
Journal ID: ISSN 0003-2700; 453040220
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry; Journal Volume: 90; Journal Issue: 8
Country of Publication:
United States
Language:
English

Citation Formats

Ouyang, Hui, Lu, Qian, Wang, Wenwen, Song, Yang, Tu, Xinman, Zhu, Chengzhou, Smith, Jordan N., Du, Dan, Fu, Zhifeng, and Lin, Yuehe. Dual-Readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe. United States: N. p., 2018. Web. doi:10.1021/acs.analchem.7b05247.
Ouyang, Hui, Lu, Qian, Wang, Wenwen, Song, Yang, Tu, Xinman, Zhu, Chengzhou, Smith, Jordan N., Du, Dan, Fu, Zhifeng, & Lin, Yuehe. Dual-Readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe. United States. doi:10.1021/acs.analchem.7b05247.
Ouyang, Hui, Lu, Qian, Wang, Wenwen, Song, Yang, Tu, Xinman, Zhu, Chengzhou, Smith, Jordan N., Du, Dan, Fu, Zhifeng, and Lin, Yuehe. Tue . "Dual-Readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe". United States. doi:10.1021/acs.analchem.7b05247.
@article{osti_1437545,
title = {Dual-Readout Immunochromatographic Assay by Utilizing MnO2 Nanoflowers as the Unique Colorimetric/Chemiluminescent Probe},
author = {Ouyang, Hui and Lu, Qian and Wang, Wenwen and Song, Yang and Tu, Xinman and Zhu, Chengzhou and Smith, Jordan N. and Du, Dan and Fu, Zhifeng and Lin, Yuehe},
abstractNote = {Manganese dioxide nanoflowers (MnO2 NFs) were synthesized and utilized as a dual readout probe to develop a novel immunochromatographic test strip (ITS) for detecting pesticide residues using chlorpyrifos as the model analyte. MnO2 NFs-labeled antibody for chlorpyrifos was employed as the signal tracer for conducting the ITS. After 10-min competitive immunoreaction, the tracer antibody was captured by the immobilized immunogen on test line in the test strip, resulting in the accumulation of MnO2 NFs. The accumulation of MnO2 NFs led to the appearance of brown color on the test line, which could be easily observed by the naked eye as a qualitative readout. Moreover, MnO2 NFs showed a remarkably enhancing effect on the luminol-H2O2 chemiluminescent (CL) system. Unlike peroxidase-like nanomaterials, the enhancing mechanism of MnO2 NFs was based on its oxidant activity to decompose H2O2 for forming reactive oxygen species. After initiating the CL system in the test zone, strong CL signal was collected as a quantitative readout to sensitively detect chlorpyrifos. Under optimal conditions, the linear range of chlorpyrifos was 0.1–50 ng/mL with a low detection limit of 0.033 ng/mL (S/N = 3). The reliability of the dual-readout ITS was successfully demonstrated by the application on traditional Chinese medicine and environmental water samples. Due to the simultaneous rapid-qualitative and sensitive-quantitative detection, the dual-readout protocol provides a promising strategy for rapid screening and field assay on various areas such as environmental monitoring, food safety and point-of-care testing.},
doi = {10.1021/acs.analchem.7b05247},
journal = {Analytical Chemistry},
number = 8,
volume = 90,
place = {United States},
year = {Tue Mar 27 00:00:00 EDT 2018},
month = {Tue Mar 27 00:00:00 EDT 2018}
}