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Title: Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks

Abstract

Hybrids of protein biopolymers and synthetic polymers are a promising new class of soft materials, as the advantages of each component can be complementary. A recombinant elastin-like polypeptide (ELP) was conjugated to poly(ethylene glycol) (PEG) by macromolecular coupling in solution to form multiblock ELP-PEG copolymers. The hydrated copolymer preserved the thermoresponsive properties from the ELP block and formed hydrogels with different transition temperatures depending on salt concentration. Small angle scattering indicates that the copolymer hydrogels form sphere-like aggregates with a “fuzzy” interface, while the films form a fractal network of nanoscale aggregates. Here, the use of solutions with different salt concentrations to prepare the hydrogels was found to influence the transition temperature, the mechanical properties, and the size of the nanoscale structure of the hydrogel without changing the secondary structure of the ELP. The salt variation and the addition of a plasticizer also affected the nanoscale structure and the mechanical characteristics of the films.

Authors:
 [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Univ. of Minho, Guimarães (Portugal)
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Science Foundation (NSF)
OSTI Identifier:
1437484
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biomacromolecules
Additional Journal Information:
Journal Volume: 19; Journal Issue: 2; Journal ID: ISSN 1525-7797
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; thin films; amides; peptides and proteins; copolymers; hydrogels; PEG; ELP; physical networks

Citation Formats

Araújo, Andreia, Olsen, Bradley D., and Machado, Ana Vera. Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks. United States: N. p., 2017. Web. doi:10.1021/acs.biomac.7b01424.
Araújo, Andreia, Olsen, Bradley D., & Machado, Ana Vera. Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks. United States. doi:10.1021/acs.biomac.7b01424.
Araújo, Andreia, Olsen, Bradley D., and Machado, Ana Vera. Mon . "Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks". United States. doi:10.1021/acs.biomac.7b01424. https://www.osti.gov/servlets/purl/1437484.
@article{osti_1437484,
title = {Engineering Elastin-Like Polypeptide-Poly(ethylene glycol) Multiblock Physical Networks},
author = {Araújo, Andreia and Olsen, Bradley D. and Machado, Ana Vera},
abstractNote = {Hybrids of protein biopolymers and synthetic polymers are a promising new class of soft materials, as the advantages of each component can be complementary. A recombinant elastin-like polypeptide (ELP) was conjugated to poly(ethylene glycol) (PEG) by macromolecular coupling in solution to form multiblock ELP-PEG copolymers. The hydrated copolymer preserved the thermoresponsive properties from the ELP block and formed hydrogels with different transition temperatures depending on salt concentration. Small angle scattering indicates that the copolymer hydrogels form sphere-like aggregates with a “fuzzy” interface, while the films form a fractal network of nanoscale aggregates. Here, the use of solutions with different salt concentrations to prepare the hydrogels was found to influence the transition temperature, the mechanical properties, and the size of the nanoscale structure of the hydrogel without changing the secondary structure of the ELP. The salt variation and the addition of a plasticizer also affected the nanoscale structure and the mechanical characteristics of the films.},
doi = {10.1021/acs.biomac.7b01424},
journal = {Biomacromolecules},
issn = {1525-7797},
number = 2,
volume = 19,
place = {United States},
year = {2017},
month = {12}
}

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Works referencing / citing this record:

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
journal, June 2019