Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Yao, Lan; Yang, Haitao; Yoo, Chang Geun; Meng, Xianzhi; Pu, Yunqiao; Hao, Naijia; Ragauskas, Arthur J.

doi:10.3389/fenrg.2018.00001

Title: Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Journal Article · Tue Feb 06 00:00:00 EST 2018 · Frontiers in Energy Research

DOI:https://doi.org/10.3389/fenrg.2018.00001· OSTI ID:1437196

Yao, Lan; Yang, Haitao; Yoo, Chang Geun; Meng, Xianzhi; Pu, Yunqiao; Hao, Naijia; Ragauskas, Arthur J.

To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanol-extractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1437196

Alternate ID(s):: OSTI ID: 1436928

Journal Information:: Frontiers in Energy Research, Journal Name: Frontiers in Energy Research Vol. 6; ISSN 2296-598X

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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Cited by: 15 works

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