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Title: The role of electron irradiation history in liquid cell transmission electron microscopy

Abstract

In situ liquid cell transmission electron microscopy (LC-TEM) allows dynamic nanoscale characterization of systems in a hydrated state. Although powerful, this technique remains impaired by issues of repeatability that limit experimental fidelity and hinder the identification and control of some variables underlying observed dynamics. We detail new LC- TEM devices that improve experimental reproducibility by expanding available imaging area and providing a platform for investigating electron flux history on the sample. Irradiation history is an important factor influencing LC-TEM results that has, to this point, been largely qualitatively and not quantitatively described. We use these devices to highlight the role of cumulative electron flux history on samples from both nanoparticle growth and biological imaging experiments and demonstrate capture of time zero, low-dose images on beam-sensitive samples. In particular, the ability to capture pristine images of biological samples, where the acquired image is the first time that the cell experiences significant electron flux, allowed us to determine that nanoparticle movement compared to the cell membrane was a function of cell damage and therefore an artifact rather than visualizing cell dynamics in action. These results highlight just a subset of the new science that is accessible with LC-TEM through the new multiwindowmore » devices with patterned focusing aides.« less

Authors:
ORCiD logo; ; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1436842
Report Number(s):
PNNL-SA-120688
Journal ID: ISSN 2375-2548; 49134; 49612; KP1704020; KP1606010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Science Advances; Journal Volume: 4; Journal Issue: 4
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Moser, Trevor H., Mehta, Hardeep, Park, Chiwoo, Kelly, Ryan T., Shokuhfar, Tolou, and Evans, James E. The role of electron irradiation history in liquid cell transmission electron microscopy. United States: N. p., 2018. Web. doi:10.1126/sciadv.aaq1202.
Moser, Trevor H., Mehta, Hardeep, Park, Chiwoo, Kelly, Ryan T., Shokuhfar, Tolou, & Evans, James E. The role of electron irradiation history in liquid cell transmission electron microscopy. United States. doi:10.1126/sciadv.aaq1202.
Moser, Trevor H., Mehta, Hardeep, Park, Chiwoo, Kelly, Ryan T., Shokuhfar, Tolou, and Evans, James E. Sun . "The role of electron irradiation history in liquid cell transmission electron microscopy". United States. doi:10.1126/sciadv.aaq1202.
@article{osti_1436842,
title = {The role of electron irradiation history in liquid cell transmission electron microscopy},
author = {Moser, Trevor H. and Mehta, Hardeep and Park, Chiwoo and Kelly, Ryan T. and Shokuhfar, Tolou and Evans, James E.},
abstractNote = {In situ liquid cell transmission electron microscopy (LC-TEM) allows dynamic nanoscale characterization of systems in a hydrated state. Although powerful, this technique remains impaired by issues of repeatability that limit experimental fidelity and hinder the identification and control of some variables underlying observed dynamics. We detail new LC- TEM devices that improve experimental reproducibility by expanding available imaging area and providing a platform for investigating electron flux history on the sample. Irradiation history is an important factor influencing LC-TEM results that has, to this point, been largely qualitatively and not quantitatively described. We use these devices to highlight the role of cumulative electron flux history on samples from both nanoparticle growth and biological imaging experiments and demonstrate capture of time zero, low-dose images on beam-sensitive samples. In particular, the ability to capture pristine images of biological samples, where the acquired image is the first time that the cell experiences significant electron flux, allowed us to determine that nanoparticle movement compared to the cell membrane was a function of cell damage and therefore an artifact rather than visualizing cell dynamics in action. These results highlight just a subset of the new science that is accessible with LC-TEM through the new multiwindow devices with patterned focusing aides.},
doi = {10.1126/sciadv.aaq1202},
journal = {Science Advances},
number = 4,
volume = 4,
place = {United States},
year = {Sun Apr 01 00:00:00 EDT 2018},
month = {Sun Apr 01 00:00:00 EDT 2018}
}