Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls

Sun, Yingyuan; Roznowski, Aaron P.; Tokuda, Joshua M.; Klose, Thomas; Mauney, Alexander; Pollack, Lois; Fane, Bentley A.; Rossmann, Michael G.

doi:10.1073/pnas.1716614114

Title: Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls

Journal Article · Mon Dec 11 00:00:00 EST 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1716614114· OSTI ID:1436803

Sun, Yingyuan ^[1]; Roznowski, Aaron P. ^[2]; Tokuda, Joshua M. ^[3];

^[1]; Mauney, Alexander ^[3]; Pollack, Lois ^[3]; Fane, Bentley A. ^[2]; Rossmann, Michael G. ^[1]

Purdue Univ., West Lafayette, IN (United States)
Univ. of Arizona, Tucson, AZ (United States)
Cornell Univ., Ithaca, NY (United States)

Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection states. The ejection is initiated by the loss of the G protein spike that encounters the LPS, followed by conformational changes of two polypeptide loops on the major capsid F proteins. One of these loops mediates viral attachment, and the other participates in making the fivefold channel at the vertex contacting the LPS.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Science Foundation (NSF); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1436803

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 52; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 25 works

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