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Title: Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA

Abstract

The spliceosome removes introns from precursor messenger RNA (pre-mRNA) to produce mature mRNA. Prior to catalysis, spliceosomes are assembled de novo onto pre-mRNA substrates. During this assembly process, U6 small nuclear RNA (snRNA) undergoes extensive structural remodeling. The early stages of this remodeling process are chaperoned by U6 snRNP proteins Prp24 and the Lsm2–8 heteroheptameric ring. We now report a structure of the U6 snRNP from Saccharomyces cerevisiae. The structure reveals protein–protein contacts that position Lsm2–8 in close proximity to the chaperone “active site” of Prp24. The structure also shows how the Lsm2–8 ring specifically recognizes U6 snRNA that has been post-transcriptionally modified at its 3' end, thereby elucidating the mechanism by which U6 snRNPs selectively recruit 3' end-processed U6 snRNA into spliceosomes. Additionally, the structure reveals unanticipated homology between the C-terminal regions of Lsm8 and the cytoplasmic Lsm1 protein involved in mRNA decay.

Authors:
 [1];  [1];  [1];  [1];  [1]; ORCiD logo [1]
  1. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Scientific User Facilities Division; National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1436775
Grant/Contract Number:  
AC02-06CH11357; P41 GM103403; S10 RR029205 085P1000817; R01 GM065166; R35 GM118131; R35 GM118075; BIR-9512577; S10RR13790
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; RNA; X-ray crystallography

Citation Formats

Montemayor, Eric J., Didychuk, Allison L., Yake, Allyson D., Sidhu, Gurnimrat K., Brow, David A., and Butcher, Samuel E. Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA. United States: N. p., 2018. Web. doi:10.1038/s41467-018-04145-4.
Montemayor, Eric J., Didychuk, Allison L., Yake, Allyson D., Sidhu, Gurnimrat K., Brow, David A., & Butcher, Samuel E. Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA. United States. doi:10.1038/s41467-018-04145-4.
Montemayor, Eric J., Didychuk, Allison L., Yake, Allyson D., Sidhu, Gurnimrat K., Brow, David A., and Butcher, Samuel E. Tue . "Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA". United States. doi:10.1038/s41467-018-04145-4. https://www.osti.gov/servlets/purl/1436775.
@article{osti_1436775,
title = {Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA},
author = {Montemayor, Eric J. and Didychuk, Allison L. and Yake, Allyson D. and Sidhu, Gurnimrat K. and Brow, David A. and Butcher, Samuel E.},
abstractNote = {The spliceosome removes introns from precursor messenger RNA (pre-mRNA) to produce mature mRNA. Prior to catalysis, spliceosomes are assembled de novo onto pre-mRNA substrates. During this assembly process, U6 small nuclear RNA (snRNA) undergoes extensive structural remodeling. The early stages of this remodeling process are chaperoned by U6 snRNP proteins Prp24 and the Lsm2–8 heteroheptameric ring. We now report a structure of the U6 snRNP from Saccharomyces cerevisiae. The structure reveals protein–protein contacts that position Lsm2–8 in close proximity to the chaperone “active site” of Prp24. The structure also shows how the Lsm2–8 ring specifically recognizes U6 snRNA that has been post-transcriptionally modified at its 3' end, thereby elucidating the mechanism by which U6 snRNPs selectively recruit 3' end-processed U6 snRNA into spliceosomes. Additionally, the structure reveals unanticipated homology between the C-terminal regions of Lsm8 and the cytoplasmic Lsm1 protein involved in mRNA decay.},
doi = {10.1038/s41467-018-04145-4},
journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {5}
}

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Cited by: 6 works
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