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Title: Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries

Abstract

Complete hydrolysis of cellulose has been noted as a key characteristic of biomass technology due to the limitation of industrial production hosts to use cellodextrin, the partial hydrolysis product of cellulose. Cellobiose, a β-1,4-linked glucose dimer, is a major cellodextrin of the enzymatic hydrolysis (via endoglucanase and exoglucanase) of cellulose. Conversion of cellobiose to glucose is executed by β-glucosidase. The complete extracellular hydrolysis of celluloses has several critical barriers in biomass technology. An alternative bioengineering strategy to make the bioprocessing less challenging is to engineer microbes with the abilities to hydrolyze and assimilate the cellulosic-hydrolysate cellodextrin. Microorganisms engineered to metabolize cellobiose rather than the monomeric glucose can provide several advantages for lignocellulose-based biorefineries. This review describes the recent advances and challenges in engineering efficient intracellular cellobiose metabolism in industrial hosts. This review also describes the limitations of and future prospectives in engineering intracellular cellobiose metabolism.

Authors:
 [1];  [1];  [2];  [1];  [3]
  1. Ulsan National Institute of Science and Technology (UNIST), Ulsan (Korea, Republic of)
  2. Joint BioEnergy Inst., Emeryville, CA (United States)
  3. Joint BioEnergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Technical Univ. of Denmark, Horsholm (Denmark). Novo Nordisk Foundation Center for Biosustainability; Synthetic Biology Engineering Research Center (Synberc), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1436614
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bioresource Technology; Journal Volume: 239; Journal Issue: C
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Parisutham, Vinuselvi, Chandran, Sathesh-Prabu, Mukhopadhyay, Aindrila, Lee, Sung Kuk, and Keasling, Jay D. Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries. United States: N. p., 2017. Web. doi:10.1016/j.biortech.2017.05.001.
Parisutham, Vinuselvi, Chandran, Sathesh-Prabu, Mukhopadhyay, Aindrila, Lee, Sung Kuk, & Keasling, Jay D. Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries. United States. doi:10.1016/j.biortech.2017.05.001.
Parisutham, Vinuselvi, Chandran, Sathesh-Prabu, Mukhopadhyay, Aindrila, Lee, Sung Kuk, and Keasling, Jay D. Thu . "Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries". United States. doi:10.1016/j.biortech.2017.05.001.
@article{osti_1436614,
title = {Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries},
author = {Parisutham, Vinuselvi and Chandran, Sathesh-Prabu and Mukhopadhyay, Aindrila and Lee, Sung Kuk and Keasling, Jay D.},
abstractNote = {Complete hydrolysis of cellulose has been noted as a key characteristic of biomass technology due to the limitation of industrial production hosts to use cellodextrin, the partial hydrolysis product of cellulose. Cellobiose, a β-1,4-linked glucose dimer, is a major cellodextrin of the enzymatic hydrolysis (via endoglucanase and exoglucanase) of cellulose. Conversion of cellobiose to glucose is executed by β-glucosidase. The complete extracellular hydrolysis of celluloses has several critical barriers in biomass technology. An alternative bioengineering strategy to make the bioprocessing less challenging is to engineer microbes with the abilities to hydrolyze and assimilate the cellulosic-hydrolysate cellodextrin. Microorganisms engineered to metabolize cellobiose rather than the monomeric glucose can provide several advantages for lignocellulose-based biorefineries. This review describes the recent advances and challenges in engineering efficient intracellular cellobiose metabolism in industrial hosts. This review also describes the limitations of and future prospectives in engineering intracellular cellobiose metabolism.},
doi = {10.1016/j.biortech.2017.05.001},
journal = {Bioresource Technology},
number = C,
volume = 239,
place = {United States},
year = {Thu May 04 00:00:00 EDT 2017},
month = {Thu May 04 00:00:00 EDT 2017}
}