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Title: Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Abstract

Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3]
  1. Zhejiang Univ., Hangzhou (China). Key Lab. of Biomass Chemical Engineering of Ministry of Education and College of Chemical and Biological Engineering; Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering and Carl R. Woese Inst. for Genomic Biology
  2. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering and Carl R. Woese Inst. for Genomic Biology
  3. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemical and Biomolecular Engineering, Carl R. Woese Inst. for Genomic Biology, and Dept. of Chemistry, Biochemistry and Bioengineering
Publication Date:
Research Org.:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Zhejiang Univ., Hangzhou (China)
OSTI Identifier:
1436426
Alternate Identifier(s):
OSTI ID: 1433560; OSTI ID: 1435935
Grant/Contract Number:  
SC0018420; SC0018260
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1860-6768
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; gene regulation; genome engineering; metabolic engineering; saccaromyces cerevisiae; synthetic biology; Saccharomyces cerevisiae

Citation Formats

Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States: N. p., 2018. Web. doi:10.1002/biot.201700601.
Lian, Jiazhang, HamediRad, Mohammad, & Zhao, Huimin. Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System. United States. doi:10.1002/biot.201700601.
Lian, Jiazhang, HamediRad, Mohammad, and Zhao, Huimin. Wed . "Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System". United States. doi:10.1002/biot.201700601.
@article{osti_1436426,
title = {Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System},
author = {Lian, Jiazhang and HamediRad, Mohammad and Zhao, Huimin},
abstractNote = {Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.},
doi = {10.1002/biot.201700601},
journal = {Biotechnology Journal},
number = 9,
volume = 13,
place = {United States},
year = {Wed Apr 18 00:00:00 EDT 2018},
month = {Wed Apr 18 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on February 13, 2019
Publisher's Version of Record

Citation Metrics:
Cited by: 3 works
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Works referenced in this record:

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