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Title: Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Abstract

Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1436069
Report Number(s):
NREL/CH-5100-71450
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; amino acids; nitrogen analysis; nitrogen-to-protein conversion factor; protein

Citation Formats

Laurens, Lieve M, Olstad-Thompson, Jessica L, and Templeton, David W. Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. United States: N. p., 2018. Web. doi:10.1007/7651_2018_126.
Laurens, Lieve M, Olstad-Thompson, Jessica L, & Templeton, David W. Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor. United States. doi:10.1007/7651_2018_126.
Laurens, Lieve M, Olstad-Thompson, Jessica L, and Templeton, David W. Fri . "Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor". United States. doi:10.1007/7651_2018_126.
@article{osti_1436069,
title = {Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor},
author = {Laurens, Lieve M and Olstad-Thompson, Jessica L and Templeton, David W},
abstractNote = {Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.},
doi = {10.1007/7651_2018_126},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Book:
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Works referenced in this record:

Protein measurements of microalgal and cyanobacterial biomass
journal, October 2010

  • González López, Cynthia Victoria; García, María del Carmen Cerón; Fernández, Francisco Gabriel Acién
  • Bioresource Technology, Vol. 101, Issue 19
  • DOI: 10.1016/J.Biortech.2010.04.077

Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern
journal, December 1883

  • Kjeldahl, J.
  • Fresenius' Zeitschrift für analytische Chemie, Vol. 22, Issue 1
  • DOI: 10.1007/BF01338151

A dye binding method for measurement of total protein in microalgae
journal, February 2012

  • Servaites, Jerome C.; Faeth, Julia L.; Sidhu, Sukh S.
  • Analytical Biochemistry, Vol. 421, Issue 1
  • DOI: 10.1016/j.ab.2011.10.047

Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition
journal, May 2014

  • Laurens, Lieve M. L.; Van Wychen, Stefanie; McAllister, Jordan P.
  • Analytical Biochemistry, Vol. 452
  • DOI: 10.1016/j.ab.2014.02.009

Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors
journal, February 2004

  • Lourenço, Sergio O.; Barbarino, Elisabete; Lavín, Paris L.
  • European Journal of Phycology, Vol. 39, Issue 1
  • DOI: 10.1080/0967026032000157156

Distribution of Intracellular Nitrogen in Marine Microalgae: Basis for the Calculation of Specific Nitrogen-To-Protein Conversion Factors
journal, October 1998


Amino acid composition, protein content and calculation of nitrogen-to-protein conversion factors for 19 tropical seaweeds
journal, September 2002


Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel
journal, September 2015


Highly valuable microalgae: biochemical and topological aspects
journal, May 2013

  • Pignolet, Olivier; Jubeau, Sébastien; Vaca-Garcia, Carlos
  • Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 8
  • DOI: 10.1007/s10295-013-1281-7

Nitrogen measurement in biological fluids and bone by an automatic Dumas technique
journal, August 1966


A rapid and general method for measurement of protein in micro-algal biomass
journal, February 2013


Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions
journal, March 1986