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Title: Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor

Book ·
ORCiD logo [1];  [1]; ORCiD logo [1]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
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Accurately determining protein content is important in the valorization of algal biomass in food, feed, and fuel markets, where these values are used for component balance calculations. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The methodology reported here covers the quantitative assessment of the total nitrogen content of algal biomass and a description of the methodology that underpins the accurate de novo calculation of a dedicated nitrogen-to-protein conversion factor.

Research Organization:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
DOE Contract Number:
AC36-08GO28308
OSTI ID:
1436069
Report Number(s):
NREL/CH-5100-71450
Country of Publication:
United States
Language:
English

References (13)

Protein measurements of microalgal and cyanobacterial biomass journal October 2010
Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern journal December 1883
A dye binding method for measurement of total protein in microalgae journal February 2012
Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition journal May 2014
Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors journal February 2004
Distribution of Intracellular Nitrogen in Marine Microalgae: Basis for the Calculation of Specific Nitrogen-To-Protein Conversion Factors journal October 1998
Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content journal January 1990
Amino acid composition, protein content and calculation of nitrogen-to-protein conversion factors for 19 tropical seaweeds journal September 2002
Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel journal September 2015
Highly valuable microalgae: biochemical and topological aspects journal May 2013
Nitrogen measurement in biological fluids and bone by an automatic Dumas technique journal August 1966
A rapid and general method for measurement of protein in micro-algal biomass journal February 2013
Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions journal March 1986

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09 BIOMASS FUELS
amino acids
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nitrogen-to-protein conversion factor
protein