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Title: Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB

Abstract

Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. In this work, we determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

Authors:
 [1];  [2];  [2];  [2];  [1]
  1. San Francisco State Univ., CA (United States)
  2. Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States); Center for Structural Genomics of Infectious Diseases (CSGID), Chicago, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
Startup Funds from San Francisco State University; National Institute of Allergy and Infectious Diseases (NIAID); National Institutes of Health (NIH); US Department of Health and Human Services (HHS)
OSTI Identifier:
1435826
Grant/Contract Number:  
HHSN272200700058C; HHSN272201200026C
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Structural Biology
Additional Journal Information:
Journal Volume: 202; Journal Issue: 2; Journal ID: ISSN 1047-8477
Publisher:
Elsevier
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Cell wall polysaccharide producing enzymes; Co-crystallization of metabolic enzymes; Dehydratase; Protein crystallization; Therapeutic drug target

Citation Formats

Gokey, Trevor, Halavaty, Andrei S., Minasov, George, Anderson, Wayne F., and Kuhn, Misty L. Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB. United States: N. p., 2018. Web. doi:10.1016/j.jsb.2018.01.006.
Gokey, Trevor, Halavaty, Andrei S., Minasov, George, Anderson, Wayne F., & Kuhn, Misty L. Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB. United States. doi:10.1016/j.jsb.2018.01.006.
Gokey, Trevor, Halavaty, Andrei S., Minasov, George, Anderson, Wayne F., and Kuhn, Misty L. Wed . "Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB". United States. doi:10.1016/j.jsb.2018.01.006. https://www.osti.gov/servlets/purl/1435826.
@article{osti_1435826,
title = {Structure of the Bacillus anthracis dTDP- l -rhamnose biosynthetic pathway enzyme: dTDP-α- d -glucose 4,6-dehydratase, RfbB},
author = {Gokey, Trevor and Halavaty, Andrei S. and Minasov, George and Anderson, Wayne F. and Kuhn, Misty L.},
abstractNote = {Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. In this work, we determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.},
doi = {10.1016/j.jsb.2018.01.006},
journal = {Journal of Structural Biology},
issn = {1047-8477},
number = 2,
volume = 202,
place = {United States},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
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