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Title: Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production

Abstract

Here, we have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm–expressed maize ( Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch–free background and with the wild–type plants. Each of the maize–derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more–than–trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [3];  [1];  [1];  [1]
  1. Univ. of Guelph, Guelph, ON (Canada)
  2. The Hospital for Sick Children, Toronto, ON (Canada)
  3. Univ. of North Texas, Denton, TX (United States)
Publication Date:
Research Org.:
Univ. of North Texas, Denton, TX (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1435780
Grant/Contract Number:  
SC0000797
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 3; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; starch; seed oil; biomass; amylopectin; starch branching enzymes

Citation Formats

Liu, Fushan, Zhao, Qianru, Mano, Noel, Ahmed, Zaheer, Nitschke, Felix, Cai, Yinqqi, Chapman, Kent D., Steup, Martin, Tetlow, Ian J., and Emes, Michael J. Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production. United States: N. p., 2015. Web. doi:10.1111/pbi.12453.
Liu, Fushan, Zhao, Qianru, Mano, Noel, Ahmed, Zaheer, Nitschke, Felix, Cai, Yinqqi, Chapman, Kent D., Steup, Martin, Tetlow, Ian J., & Emes, Michael J. Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production. United States. doi:10.1111/pbi.12453.
Liu, Fushan, Zhao, Qianru, Mano, Noel, Ahmed, Zaheer, Nitschke, Felix, Cai, Yinqqi, Chapman, Kent D., Steup, Martin, Tetlow, Ian J., and Emes, Michael J. Wed . "Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production". United States. doi:10.1111/pbi.12453. https://www.osti.gov/servlets/purl/1435780.
@article{osti_1435780,
title = {Modification of starch metabolism in transgenic Arabidopsis thaliana increases plant biomass and triples oilseed production},
author = {Liu, Fushan and Zhao, Qianru and Mano, Noel and Ahmed, Zaheer and Nitschke, Felix and Cai, Yinqqi and Chapman, Kent D. and Steup, Martin and Tetlow, Ian J. and Emes, Michael J.},
abstractNote = {Here, we have identified a novel means to achieve substantially increased vegetative biomass and oilseed production in the model plant Arabidopsis thaliana. Endogenous isoforms of starch branching enzyme (SBE) were substituted by either one of the endosperm–expressed maize (Zea mays L.) branching isozymes, ZmSBEI or ZmSBEIIb. Transformants were compared with the starch–free background and with the wild–type plants. Each of the maize–derived SBEs restored starch biosynthesis but both morphology and structure of starch particles were altered. Altered starch metabolism in the transformants is associated with enhanced biomass formation and more–than–trebled oilseed production while maintaining seed oil quality. Enhanced oilseed production is primarily due to an increased number of siliques per plant whereas oil content and seed number per silique are essentially unchanged or even modestly decreased. Introduction of cereal starch branching isozymes into oilseed plants represents a potentially useful strategy to increase biomass and oilseed production in related crops and manipulate the structure and properties of leaf starch.},
doi = {10.1111/pbi.12453},
journal = {Plant Biotechnology Journal},
number = 3,
volume = 14,
place = {United States},
year = {Wed Aug 19 00:00:00 EDT 2015},
month = {Wed Aug 19 00:00:00 EDT 2015}
}

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Cited by: 4 works
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Works referenced in this record:

Plant triacylglycerols as feedstocks for the production of biofuels
journal, May 2008


Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipid droplet accumulation in plants
journal, September 2010

  • James, Christopher N.; Horn, Patrick J.; Case, Charlene R.
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