Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus

Laino, Aldana; Lopez-Zavala, Alonso A.; Garcia-Orozco, Karina D.; Carrasco-Miranda, Jesus S.; Santana, Marianela; Stojanoff, Vivian; Sotelo-Mundo, Rogerio R.; Garcia, Carlos Fernando

doi:10.7717/peerj.3787

Title: Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus

Journal Article · Mon Sep 11 00:00:00 EDT 2017 · PeerJ

DOI:https://doi.org/10.7717/peerj.3787· OSTI ID:1435730

Laino, Aldana ^[1]; Lopez-Zavala, Alonso A. ^[2]; Garcia-Orozco, Karina D. ^[3]; Carrasco-Miranda, Jesus S. ^[3]; Santana, Marianela ^[1]; Stojanoff, Vivian ^[4]; Sotelo-Mundo, Rogerio R. ^[3]; Garcia, Carlos Fernando ^[1]

Univ. Nacional de La Plata, Buenos Aires (Argentina)
Univ. de Sonora, Sonora (Mexico)
Centro de Investigacion en Alimentacion y Desarrollo, Sonora (Mexico)
Brookhaven National Lab. (BNL), Upton, NY (United States)

Energy buffering systems are key for homeostasis during variations in energy supply. Spiders are the most important predators for insects and therefore key in terrestrial ecosystems. From biomedical interest, spiders are important for their venoms and as a source of potent allergens, such as arginine kinase (AK, EC 2.7.3.3). AK is an enzyme crucial for energy metabolism, keeping the pool of phosphagens in invertebrates, and also an allergen for humans. In this work, we studied AK from the Argentininan spider Polybetes pythagoricus (PpAK), from its complementary DNA to the crystal structure. The PpAK cDNA from muscle was cloned, and it is comprised of 1068 nucleotides that encode a 384-amino acids protein, similar to other invertebrate AKs. The apparent Michaelis-Menten kinetic constant (K_m) was 1.7 mM with a k_cat of 75 s^–1. Two crystal structures are presented, the apoPvAK and PpAK bound to arginine, both in the open conformation with the active site lid (residues 310–320) completely disordered. The guanidino group binding site in the apo structure appears to be organized to accept the arginine substrate. Lastly, these results contribute to knowledge of mechanistic details of the function of arginine kinase.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704

OSTI ID:: 1435730

Report Number(s):: BNL-203458-2018-JAAM

Journal Information:: PeerJ, Vol. 5; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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36 MATERIALS SCIENCE
Arginine kinase
Spider
cDNA cloning
Crystal structure
Phosphagen
Polybetes pytagoricus
Arthropoda
Arachnida
Open conformation
Allergen

Title: Biochemical and structural characterization of a novel arginine kinase from the spider Polybetes pythagoricus

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