skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Quantitative proteome profile of water deficit stress responses in eastern cottonwood ( Populus deltoides) leaves

Abstract

Drought stress is a recurring feature of world climate and the single most important factor influencing agricultural yield worldwide. Plants display highly variable, species-specific responses to drought and these responses are multifaceted, requiring physiological and morphological changes influenced by genetic and molecular mechanisms. Moreover, the reproducibility of water deficit studies is very cumbersome, which significantly impedes research on drought tolerance, because how a plant responds is highly influenced by the timing, duration, and intensity of the water deficit. Despite progress in the identification of drought-related mechanisms in many plants, the molecular basis of drought resistance remains to be fully understood in trees, particularly in poplar species because their wide geographic distribution results in varying tolerances to drought. Herein, we aimed to better understand this complex phenomenon in eastern cottonwood ( Populus deltoides) by performing a detailed contrast of the proteome changes between two different water deficit experiments to identify functional intersections and divergences in proteome responses. We investigated plants subjected to cyclic water deficit and compared these responses to plants subjected to prolonged acute water deficit. In total, we identified 108,012 peptide sequences across both experiments that provided insight into the quantitative state of 22,737 Populus gene models and 8,199more » functional protein groups in response to drought. Together, these datasets provide the most comprehensive insight into proteome drought responses in poplar to date and a direct proteome comparison between short period dehydration shock and cyclic, post-drought re-watering. Altogether, this investigation provides novel insights into drought avoidance mechanisms that are distinct from progressive drought stress. Additionally, we identified proteins that have been associated as drought-relevant in previous studies. Importantly, we highlight the RD26 transcription factor as a gene regulated at both the transcript and protein level, regardless of species and drought condition, and, thus, represents a key, universal drought marker for Populus species.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [2]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Chinese Academy of Sciences (CAS), Beijing (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1435308
Grant/Contract Number:
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 13; Journal Issue: 2; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; water resources; gene ontologies; leaves; proteomes; plant resistance to abiotic stress; transcription factors; poplars; drought adaptation

Citation Formats

Abraham, Paul E., Garcia, Benjamin J., Gunter, Lee E., Jawdy, Sara S., Engle, Nancy L., Yang, Xiaohan, Jacobson, Daniel A., Hettich, Robert L., Tuskan, Gerald A., Tschaplinski, Timothy J., and Zhang, Jin -Song. Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves. United States: N. p., 2018. Web. doi:10.1371/journal.pone.0190019.
Abraham, Paul E., Garcia, Benjamin J., Gunter, Lee E., Jawdy, Sara S., Engle, Nancy L., Yang, Xiaohan, Jacobson, Daniel A., Hettich, Robert L., Tuskan, Gerald A., Tschaplinski, Timothy J., & Zhang, Jin -Song. Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves. United States. doi:10.1371/journal.pone.0190019.
Abraham, Paul E., Garcia, Benjamin J., Gunter, Lee E., Jawdy, Sara S., Engle, Nancy L., Yang, Xiaohan, Jacobson, Daniel A., Hettich, Robert L., Tuskan, Gerald A., Tschaplinski, Timothy J., and Zhang, Jin -Song. Thu . "Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves". United States. doi:10.1371/journal.pone.0190019. https://www.osti.gov/servlets/purl/1435308.
@article{osti_1435308,
title = {Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves},
author = {Abraham, Paul E. and Garcia, Benjamin J. and Gunter, Lee E. and Jawdy, Sara S. and Engle, Nancy L. and Yang, Xiaohan and Jacobson, Daniel A. and Hettich, Robert L. and Tuskan, Gerald A. and Tschaplinski, Timothy J. and Zhang, Jin -Song},
abstractNote = {Drought stress is a recurring feature of world climate and the single most important factor influencing agricultural yield worldwide. Plants display highly variable, species-specific responses to drought and these responses are multifaceted, requiring physiological and morphological changes influenced by genetic and molecular mechanisms. Moreover, the reproducibility of water deficit studies is very cumbersome, which significantly impedes research on drought tolerance, because how a plant responds is highly influenced by the timing, duration, and intensity of the water deficit. Despite progress in the identification of drought-related mechanisms in many plants, the molecular basis of drought resistance remains to be fully understood in trees, particularly in poplar species because their wide geographic distribution results in varying tolerances to drought. Herein, we aimed to better understand this complex phenomenon in eastern cottonwood (Populus deltoides) by performing a detailed contrast of the proteome changes between two different water deficit experiments to identify functional intersections and divergences in proteome responses. We investigated plants subjected to cyclic water deficit and compared these responses to plants subjected to prolonged acute water deficit. In total, we identified 108,012 peptide sequences across both experiments that provided insight into the quantitative state of 22,737 Populus gene models and 8,199 functional protein groups in response to drought. Together, these datasets provide the most comprehensive insight into proteome drought responses in poplar to date and a direct proteome comparison between short period dehydration shock and cyclic, post-drought re-watering. Altogether, this investigation provides novel insights into drought avoidance mechanisms that are distinct from progressive drought stress. Additionally, we identified proteins that have been associated as drought-relevant in previous studies. Importantly, we highlight the RD26 transcription factor as a gene regulated at both the transcript and protein level, regardless of species and drought condition, and, thus, represents a key, universal drought marker for Populus species.},
doi = {10.1371/journal.pone.0190019},
journal = {PLoS ONE},
number = 2,
volume = 13,
place = {United States},
year = {Thu Feb 15 00:00:00 EST 2018},
month = {Thu Feb 15 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share: