Accessing the Vibrational Signatures of Amino Acid Ions Embedded in Water Clusters
We present an infrared predissociation (IRPD) study of microsolvated GlyH+(H2O)n and GlyH+(D2O)n clusters, formed inside of a cryogenic ion trap via condensation of H2O or D2O onto the protonated glycine ions. The resulting IRPD spectra, showing characteristic O–H and O–D stretches, indicate that H/D exchange reactions are quenched when the ion trap is held at 80 K, minimizing the presence of isotopomers. Comparisons of GlyH+(H2O)n and GlyH+(D2O)n spectra clearly highlight and distinguish the vibrational signatures of the water solvent molecules from those of the core GlyH+ ion, allowing for quick assessment of solvation structures. Without the aid of calculations, we can already infer solvation motifs and the presence of multiple conformations. Furthermore, the use of a cryogenic ion trap to cluster solvent molecules around ions of interest and control H/D exchange reactions is broadly applicable and should be extendable to studies of more complex peptidic ions in large solvated clusters.
- Research Organization:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Grant/Contract Number:
- SC0010326
- OSTI ID:
- 1434670
- Journal Information:
- Journal of Physical Chemistry Letters, Vol. 9; ISSN 1948-7185
- Publisher:
- American Chemical SocietyCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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