skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Variants of cellobiohydrolases

Abstract

Disclosed are a number of homologs and variants of Hypocrea jecorina Ce17A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.

Inventors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
DANISCO US INC, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1434527
Patent Number(s):
9,938,551
Application Number:
14/650,285
Assignee:
DANISCO US INC (CA) GFO
DOE Contract Number:  
FC36-08GO18078
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Dec 10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bott, Richard R., Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Nikolaev, Igor, Sandgren, Mats, Van Lieshout, Johannes Franciscus Thomas, and Van Stigt Thans, Sander. Variants of cellobiohydrolases. United States: N. p., 2018. Web.
Bott, Richard R., Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Nikolaev, Igor, Sandgren, Mats, Van Lieshout, Johannes Franciscus Thomas, & Van Stigt Thans, Sander. Variants of cellobiohydrolases. United States.
Bott, Richard R., Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Nikolaev, Igor, Sandgren, Mats, Van Lieshout, Johannes Franciscus Thomas, and Van Stigt Thans, Sander. Tue . "Variants of cellobiohydrolases". United States. https://www.osti.gov/servlets/purl/1434527.
@article{osti_1434527,
title = {Variants of cellobiohydrolases},
author = {Bott, Richard R. and Foukaraki, Maria and Hommes, Ronaldus Wilhelmus and Kaper, Thijs and Kelemen, Bradley R. and Kralj, Slavko and Nikolaev, Igor and Sandgren, Mats and Van Lieshout, Johannes Franciscus Thomas and Van Stigt Thans, Sander},
abstractNote = {Disclosed are a number of homologs and variants of Hypocrea jecorina Ce17A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Molecular Cloning and Characterization of cel2 from the Fungus Cochlibolus carbonum
journal, January 2001

  • Ahn, Joong-Hoon; Sposato, Paola; Kim, Su-Il
  • Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 6, p. 1406-1411
  • DOI: 10.1271/bbb.65.1406

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001

  • Aro, Nina; Saloheimo, Anu; Ilm�n, Marja
  • Journal of Biological Chemistry, Vol. 276, Issue 26, p. 24309-24314
  • DOI: 10.1074/jbc.M003624200

Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor.
journal, September 1991

  • Bajar, A.; Podila, G. K.; Kolattukudy, P. E.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 18, p. 8208-8212
  • DOI: 10.1073/pnas.88.18.8208

Transformation of Aspergillus nidulans by the orotidine-5?-phosphate decarboxylase gene of Neurospora crassa
journal, April 1983

  • Ballance, D. J.; Buxton, F. P.; Turner, G.
  • Biochemical and Biophysical Research Communications, Vol. 112, Issue 1, p. 284-289
  • DOI: 10.1016/0006-291X(83)91828-4

Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes
journal, May 1991

  • Berg�s, Thierry; Barreau, Christian
  • Current Genetics, Vol. 19, Issue 5, p. 359-365
  • DOI: 10.1007/BF00309596

Fractionation of cellulase and ?-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999

  • Brumbauer, Anik�; Johansson, G�te; R�czey, Kati
  • Bioseparation, Vol. 7, Issue 6, p. 287-295
  • DOI: 10.1023/A:1008153418019

Aromatic-aromatic interaction: a mechanism of protein structure stabilization
journal, July 1985


Randomization of genes by PCR mutagenesis.
journal, August 1992

  • Cadwell, R. C.; Joyce, G. F.
  • Genome Research, Vol. 2, Issue 1, p. 28-33
  • DOI: 10.1101/gr.2.1.28

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase
journal, July 1989

  • Campbell, Edward I.; Unkles, Shiela E.; Macro, Janet A.
  • Current Genetics, Vol. 16, Issue 1, p. 53-56
  • DOI: 10.1007/BF00411084

Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S 3 to k cat
journal, January 2000

  • Stubbs, Marlene; Ngo, Kenny Q.P.; Cunningham, Annie
  • Protein Science, Vol. 9, Issue 5, p. 991-1001
  • DOI: 10.1110/ps.9.5.991

Improved oligonudeotide site-directed rautagenesis using M13 vectors
journal, January 1985

  • Carter, Paul; Bedouelle, Hugues; Winter, Greg
  • Nucleic Acids Research, Vol. 13, Issue 12, p. 4431-4443
  • DOI: 10.1093/nar/13.12.4431

Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride
journal, January 1990

  • Cheng, Cheng; Tsukagoshi, Norihiro; Udaka, Shigezo
  • Nucleic Acids Research, Vol. 18, Issue 18, p. 5559-5559
  • DOI: 10.1093/nar/18.18.5559

Genomic organization of a cellulase gene family in Phanerochaete chrysosporium
journal, November 1992

  • Covert, Sarah F.; Bolduc, Jennifer; Cullen, Dan
  • Current Genetics, Vol. 22, Issue 5, p. 407-413
  • DOI: 10.1007/BF00352442

Oligosaccharide specificity of a family 7 endoglucanase: insertion of potential sugar-binding subsites
journal, September 1997

  • Davies, Gideon J.; Ducros, Val�rie; Lewis, Richard J.
  • Journal of Biotechnology, Vol. 57, Issue 1-3, p. 91-100
  • DOI: 10.1016/S0168-1656(97)00092-8

[57] Rethinking your purification procedure
book, January 1990


High-resolution crystal structures reveal how a cellulose chain is bound in the 50 Å long tunnel of cellobiohydrolase I from Trichoderma reesei
journal, January 1998

  • Divne, Christina; Ståhlberg, Jerry; Teeri, Tuula T.
  • Journal of Molecular Biology, Vol. 275, Issue 2, p. 309-325
  • DOI: 10.1006/jmbi.1997.1437

Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography
journal, January 1987


Response of a protein structure to cavity-creating mutations and its relation to the hydrophobic effect
journal, January 1992


Purification and characterization of a ?-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996

  • Filho, Edivaldo Ximenes Ferreira
  • Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5
  • DOI: 10.1139/m96-001

Characterization of cellulases by HPLC separation
journal, January 1983

  • Fliess, A.; Sch�gerl, K.
  • European Journal of Applied Microbiology and Biotechnology, Vol. 17, Issue 5, p. 314-318
  • DOI: 10.1007/BF00508028

Urea and Thermal Equilibrium Denaturation Studies on the Dimerization Domain of Escherichia coli Trp Repressor
journal, May 1997

  • Gloss, Lisa M.; Matthews, C. Robert
  • Biochemistry, Vol. 36, Issue 19, p. 5612-5623
  • DOI: 10.1021/bi970056e

Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase
journal, May 2002

  • Goedegebuur, Frits; Fowler, Timothy; Phillips, Jay
  • Current Genetics, Vol. 41, Issue 2, p. 89-98
  • DOI: 10.1007/s00294-002-0290-2

Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990

  • Goldman, G. H.; Van Montagu, M.; Herrera-Estrella, A.
  • Current Genetics, Vol. 17, Issue 2, p. 169-174
  • DOI: 10.1007/BF00312863

Characteristics of fungal cellulases
journal, January 1991


Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
journal, March 1998

  • Halld�rsd�ttir, S.; Th�r�lfsd�t, E. T.; Spilliaert, R.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 3, p. 277-284
  • DOI: 10.1007/s002530051169

Amino acid substitution matrices from protein blocks.
journal, November 1992

  • Henikoff, S.; Henikoff, J. G.
  • Proceedings of the National Academy of Sciences, Vol. 89, Issue 22, p. 10915-10919
  • DOI: 10.1073/pnas.89.22.10915

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
journal, November 1991

  • Hu, Q. J.; Bautista, C.; Edwards, G. M.
  • Molecular and Cellular Biology, Vol. 11, Issue 11, p. 5792-5799
  • DOI: 10.1128/MCB.11.11.5792

Production of fully human antibodies by transgenic mice
journal, January 1995


Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs
journal, September 1995


Replacing the complementarity-determining regions in a human antibody with those from a mouse
journal, May 1986

  • Jones, Peter T.; Dear, Paul H.; Foote, Jefferson
  • Nature, Vol. 321, Issue 6069, p. 522-525
  • DOI: 10.1038/321522a0

Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993

  • Karlin, S.; Altschul, S. F.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877
  • DOI: 10.1073/pnas.90.12.5873

Cloning and sequencing of the cDNA encoding ?-glucosidase 1 from Aspergillus aculeatus
journal, September 1996


Contribution of hydrophobic interactions to protein stability
journal, June 1988

  • Kellis, James T.; Nyberg, Kerstin; S?ail, Das?a
  • Nature, Vol. 333, Issue 6175, p. 784-786
  • DOI: 10.1038/333784a0

The crystal structure of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6 � resolution, and a comparison with related enzymes
journal, September 1997

  • Kleywegt, Gerard J.; Zou, Jin-Yu; Divne, Christina
  • Journal of Molecular Biology, Vol. 272, Issue 3, p. 383-397
  • DOI: 10.1006/jmbi.1997.1243

Cellulase families and their genes
journal, September 1987

  • Knowles, Jonathan; Lehtovaara, P�ivi; Teeri, Tuula
  • Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261
  • DOI: 10.1016/0167-7799(87)90102-8

Secretion of Trichoderma reesei ?-glucosidase by Saccharomyces cerevisiae
journal, February 1996

  • Cummings, C.; Fowler, T.
  • Current Genetics, Vol. 29, Issue 3, p. 227-233
  • DOI: 10.1007/BF02221552

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975

  • K�hler, G.; Milstein, C.
  • Nature, Vol. 256, Issue 5517, p. 495-497
  • DOI: 10.1038/256495a0

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001


Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocrea jecorina.
journal, July 1996

  • Kuhls, K.; Lieckfeldt, E.; Samuels, G. J.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 15, p. 7755-7760
  • DOI: 10.1073/pnas.93.15.7755

Rapid and efficient site-specific mutagenesis without phenotypic selection.
journal, January 1985

  • Kunkel, T. A.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 2, p. 488-492
  • DOI: 10.1073/pnas.82.2.488

Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis
journal, May 1990


Isolation and partial characterization of cellulose-degrading strain of Streptomyces sp. LX from soil
journal, March 1996


The roles and function of cellulose-binding domains
journal, September 1997


Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA
journal, October 1993

  • Lorito, M.; Hayes, C. K.; Di Pietro, A.
  • Current Genetics, Vol. 24, Issue 4, p. 349-356
  • DOI: 10.1007/BF00336788

Detection of a Stable Intermediate in the Thermal Unfolding of a Cysteine-Free Form of Dihydrofolate Reductase from Escherichia Coli
journal, August 1995

  • Luo, J.; Iwakura, M.; Matthews, C. R.
  • Biochemistry, Vol. 34, Issue 33, p. 10669-10675
  • DOI: 10.1021/bi00033a043

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.
journal, October 1987

  • Matthews, B. W.; Nicholson, H.; Becktel, W. J.
  • Proceedings of the National Academy of Sciences, Vol. 84, Issue 19, p. 6663-6667
  • DOI: 10.1073/pnas.84.19.6663

Structural and Genetic Analysis of Protein Stability
journal, June 1993


Family 7 cellobiohydrolases from Phanerochaete chrysosporium: crystal structure of the catalytic module of Cel7D (CBH58) at 1.32 Å resolution and homology models of the isozymes
journal, December 2001

  • Muñoz, Inés G.; Ubhayasekera, Wimal; Henriksson, Hongbin
  • Journal of Molecular Biology, Vol. 314, Issue 5, p. 1097-1111
  • DOI: 10.1006/jmbi.2000.5180

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970


Specific quantification of trichoderma reesei cellulases in reconstituted mixtures and its application to cellulase-cellulose binding studies
journal, October 1994

  • Nidetzky, Bernd; Claeyssens, Marc
  • Biotechnology and Bioengineering, Vol. 44, Issue 8, p. 961-966
  • DOI: 10.1002/bit.260440812

Structure of Cellulases and Their Applications
journal, April 1997

  • Ohmiya, Kunio; Sakka, Kazuo; Karita, Shuichi
  • Biotechnology and Genetic Engineering Reviews, Vol. 14, Issue 1, p. 365-414
  • DOI: 10.1080/02648725.1997.10647949

Improved tools for biological sequence comparison.
journal, April 1988

  • Pearson, W. R.; Lipman, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448
  • DOI: 10.1073/pnas.85.8.2444

Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae
journal, September 1987

  • Panttilä, Merja E.; André, Lars; Saloheimo, Markku
  • Yeast, Vol. 3, Issue 3, p. 175-185
  • DOI: 10.1002/yea.320030305

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987


Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae
journal, March 1988


Reshaping human antibodies for therapy
journal, March 1988

  • Riechmann, Lutz; Clark, Michael; Waldmann, Herman
  • Nature, Vol. 332, Issue 6162, p. 323-327
  • DOI: 10.1038/332323a0

Synthesis and secretion of wheat ?-amylase in Saccharomyces cerevisiae
journal, January 1987


Engineering thermostability: lessons from thermophilic proteins
journal, January 1995


CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora
journal, May 1990


Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae
journal, April 1995

  • Sakamoto, S.; Tamura, G.; Ito, K.
  • Current Genetics, Vol. 27, Issue 5, p. 435-439
  • DOI: 10.1007/BF00311212

EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
journal, March 1988


Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability
journal, April 2003

  • Sandgren, Mats; Gualfetti, Peter J.; Shaw, Andrew
  • Protein Science, Vol. 12, Issue 4, p. 848-860
  • DOI: 10.1110/ps.0237703

Cellulases of Trichoderma reesei
journal, January 1988


[79] Purification of all glycolytic enzymes from one muscle extract
journal, January 1982


Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
journal, July 1984

  • Sheir-Neiss, G.; Montenecourt, B. S.
  • Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53
  • DOI: 10.1007/BF00254645

Enzymic activities of endo-1,4-?-d-glucanases purified from Trichoderma viride
journal, March 1978

  • Shoemaker, S. P.; Brown, R. D.
  • Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 523, Issue 1, p. 133-146
  • DOI: 10.1016/0005-2744(78)90016-5

Comparison of biosequences
journal, December 1981


Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli
journal, September 1994

  • Spilliaert, Remi; Hreggvidsson, Gudmundur Oli; Kristjansson, Jakob Kristjan
  • European Journal of Biochemistry, Vol. 224, Issue 3, p. 923-930
  • DOI: 10.1111/j.1432-1033.1994.00923.x

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose
journal, September 1997


Structure of the Endoglucanase I from Fusarium oxysporum:  Native, Cellobiose, and 3,4-Epoxybutyl β-d-Cellobioside-Inhibited Forms, at 2.3 Å Resolution
May 1997

  • Sulzenbacher, Gerlind; Schülein, Martin; Davies, Gideon J.
  • Biochemistry, Vol. 36, Issue 19, p. 5902-5911
  • DOI: 10.1021/bi962963+

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000


Determinants of Enzyme Thermostability Observed in the Molecular Structure of Thermus aquaticus d -Glyceraldehyde-3-phosphate Dehydrogenase at 2.5 Å Resolution
journal, January 1996

  • Tanner, John J.; Hecht, Ralph M.; Krause, Kurt L.
  • Biochemistry, Vol. 35, Issue 8, p. 2597-2609
  • DOI: 10.1021/bi951988q

Crystalline cellulose degradation: new insight into the function of cellobiohydrolases
journal, May 1997


Molecular Biology of Cellulolytic Fungi
book, January 1995


Molecular Cloning of Exo�Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983

  • Shoemaker, S.; Schweickart, V.; Ladner, M.
  • Nature Biotechnology, Vol. 1, Issue 8, p. 691-696
  • DOI: 10.1038/nbt1083-691

A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
journal, March 1991

  • St�hlberg, Jerry; Johansson, Gunnar; Pettersson, G�ran
  • Nature Biotechnology, Vol. 9, Issue 3, p. 286-290
  • DOI: 10.1038/nbt0391-286

The molecular biology of the yeast saccharomyces
journal, September 1982


Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987


Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei
journal, September 2000


Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction
journal, December 1999


Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988


Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose.
journal, November 1996


Construction of mutant and chimeric genes using the polymerase chain reaction
journal, January 1989

  • Vallette, Francois; Mege, Emmanuelle; Reiss, Allison
  • Nucleic Acids Research, Vol. 17, Issue 2, p. 723-733
  • DOI: 10.1093/nar/17.2.723

Development of a homologous transformation system for Aspergillus niger based on the pyrG gene
journal, January 1987

  • van Hartingsveldt, Wim; Mattern, Ineke E.; van Zeijl, Cora M. J.
  • Molecular and General Genetics MGG, Vol. 206, Issue 1, p. 71-75
  • DOI: 10.1007/BF00326538

Engineering yeast for efficient cellulose degradation
journal, January 1998


Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984

  • van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; G�ran Pettersson, L.
  • FEBS Letters, Vol. 169, Issue 2, p. 215-218
  • DOI: 10.1016/0014-5793(84)80321-X

Reshaping human antibodies: grafting an antilysozyme activity
journal, March 1988


Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins
journal, August 1993

  • Ward, Michael; Wilson, Lori J.; Kodama, Katherine H.
  • Applied Microbiology and Biotechnology, Vol. 39, Issue 6, p. 738-743
  • DOI: 10.1007/BF00164459

Multiple Proline Substitutions Cumulatively Thermostabilize Bacillus Cereus ATCC7064 Oligo-1,6-Glucosidase. Irrefragable Proof Supporting the Proline Rule
journal, December 1994


Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985


Methods for measuring cellulase activities
journal, January 1988


Temperature adaptation of lactate dehydrogenase Structural, functional and genetic aspects
journal, February 1988


Transformation of Aspergillus nidulans by using a trpC plasmid.
journal, March 1984

  • Yelton, M. M.; Hamer, J. E.; Timberlake, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 81, Issue 5, p. 1470-1474
  • DOI: 10.1073/pnas.81.5.1470