Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

Guo, Gongrui; Fuchs, Martin R.; Shi, Wuxian; Skinner, John; Berman, Evanna; Ogata, Craig M.; Hendrickson, Wayne A.; McSweeney, Sean; Liu, Qun

doi:10.1107/S2052252518005389

Title: Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

Journal Article · Thu Apr 19 00:00:00 EDT 2018 · IUCrJ

DOI:https://doi.org/10.1107/S2052252518005389· OSTI ID:1433744

Guo, Gongrui;

; Shi, Wuxian; Skinner, John;

; Ogata, Craig M.; Hendrickson, Wayne A.; McSweeney, Sean;

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallography with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

Grant/Contract Number:: SC0012704; KC0401040; AC02-06CH11357

OSTI ID:: 1433744

Alternate ID(s):: OSTI ID: 1434774; OSTI ID: 1466304

Report Number(s):: BNL-203558-2018-JAAM; IUCRAJ; PII: S2052252518005389

Journal Information:: IUCrJ, Journal Name: IUCrJ Vol. 5 Journal Issue: 3; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

Citation Metrics:

Cited by: 27 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

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