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Title: Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

Abstract

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallography with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. Here, the method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

Authors:
 [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1];  [2];  [3];  [1]; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Columbia Univ., New York, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1433744
Alternate Identifier(s):
OSTI ID: 1434774
Report Number(s):
BNL-203558-2018-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ
Grant/Contract Number:
SC0012704; LDRD 15-034; KC0401040
Resource Type:
Journal Article: Published Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microcrystals; microdiffraction; radiation damage; data analysis; multiple crystals; X-ray crystallography; structural biology

Citation Formats

Guo, Gongrui, Fuchs, Martin R., Shi, Wuxian, Skinner, John, Berman, Evanna, Ogata, Craig M., Hendrickson, Wayne A., McSweeney, Sean, and Liu, Qun. Sample manipulation and data assembly for robust microcrystal synchrotron crystallography. United States: N. p., 2018. Web. doi:10.1107/S2052252518005389.
Guo, Gongrui, Fuchs, Martin R., Shi, Wuxian, Skinner, John, Berman, Evanna, Ogata, Craig M., Hendrickson, Wayne A., McSweeney, Sean, & Liu, Qun. Sample manipulation and data assembly for robust microcrystal synchrotron crystallography. United States. doi:10.1107/S2052252518005389.
Guo, Gongrui, Fuchs, Martin R., Shi, Wuxian, Skinner, John, Berman, Evanna, Ogata, Craig M., Hendrickson, Wayne A., McSweeney, Sean, and Liu, Qun. Thu . "Sample manipulation and data assembly for robust microcrystal synchrotron crystallography". United States. doi:10.1107/S2052252518005389.
@article{osti_1433744,
title = {Sample manipulation and data assembly for robust microcrystal synchrotron crystallography},
author = {Guo, Gongrui and Fuchs, Martin R. and Shi, Wuxian and Skinner, John and Berman, Evanna and Ogata, Craig M. and Hendrickson, Wayne A. and McSweeney, Sean and Liu, Qun},
abstractNote = {With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallography with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. Here, the method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.},
doi = {10.1107/S2052252518005389},
journal = {IUCrJ},
number = 3,
volume = 5,
place = {United States},
year = {Thu Apr 19 00:00:00 EDT 2018},
month = {Thu Apr 19 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1107/S2052252518005389

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