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Title: Ace1 prevents intracellular copper accumulation by regulating Fet3 expression and thereby restricting Aft1 activity

Journal Article · · Federation of European Biochemical Societies (FEBS) Journal
DOI:https://doi.org/10.1111/febs.14450· OSTI ID:1433430
 [1];  [1];  [1];  [1];  [1]
  1. Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de Lisboa Oeiras Portugal
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In the yeast Saccharomyces cerevisiae Aft1, the low iron‐sensing transcription factor is known to regulate the expression of the FET 3 gene. However, we found that a strain‐lacking FET 3 is more sensitive to copper excess than a strain‐lacking AFT 1 , and accordingly, FET 3 expression is not fully compromised in the latter. These findings suggest that, under such conditions, another regulator comes into play and controls FET 3 expression. In this work, we identify Ace1, the regulator of copper detoxification genes, as a regulator of FET 3 . We suggest that the activation of FET 3 by Ace1 prevents the hyper activation of Aft1, possibly by assuring the adequate functioning of mitochondrial iron–sulfur cluster biogenesis. While reinforcing the link between iron and copper homeostasis, this work unveils a novel protection mechanism against copper toxicity mediated by Ace1, which relies in the activation of FET 3 and results in the restriction of Aft1 activity as a means to prevent excessive copper accumulation.

Sponsoring Organization:
USDOE
OSTI ID:
1433430
Journal Information:
Federation of European Biochemical Societies (FEBS) Journal, Journal Name: Federation of European Biochemical Societies (FEBS) Journal Vol. 285 Journal Issue: 10; ISSN 1742-464X
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

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